Applied Microscopy

  • 제40권4호
  • /
  • Pages.245-251
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  • 2010
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  • 2287-5123(pISSN)
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  • 2287-4445(eISSN)
한국현미경학회 (Korean Society of Microscopy)
권호 표지

글리콜산을 사용한 화학적 필링이 Mice 피부 표피에 미치는 영향

Effect of Epidermal Changes in the Mice Skin Following Glycolic Acid Peeling

  • 이지연 (한서대학교 보건학부 피부미용학과) ;
  • 이석준 (단국대학교 체육대학 태권도학과) ;
  • 장병수 (한서대학교 보건학부 피부미용학과)
  • 투고 : 2010.12.06
  • 심사 : 2010.12.26
  • 발행 : 2010.12.31
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초록

본 연구는 15% 글리콜산과 30% 글리콜산이 포함된 각각의 필링제를 털이 제거된 mice 등쪽 피부에 처리하여 시술 전후의 피부 수분량, 유분량 및 pH를 Cutometer로 분석하였고 표피 각 질의 변화에 대한 미세구조를 더마스코프와 주사전자현미경을 사용하여 관찰하였다. 15% 글리콜산을 처리한 실험군 I과 30% 글리콜산을 처리한 실험군 II에서 피부의 수분량, 유분량 및 pH의 변화는 필링제의 농도가 높을수록 감소하는 것으로 확인되었다. Mice 피부의 표면을 더마스코프로 관찰한 결과 정상 대조군의 피부는 전반적으로 투명한 상태로 표피에서 각질이 분리되어 있거나 탈락되기 직전의 상태로 나타났다. 15% 글리콜산으로 처리한 mice 피부 표면의 각질은 많이 제거된 상태로 관찰되었으며 30% 글리콜산으로 처리한 mice 피부 표면은 매끄러운 상태로 선홍색을 띠고 있었다. Mice 피부 각질층을 인위적으로 탈락시켜서 주사전자현미경으로 관찰한 결과 정상 대조군의 각질층 표면은 각화세포 막의 함입에 의해서 불규칙하였다. 15% 글리콜산으로 처리한 mice 피부의 각질층 표면은 비교적 매끄러웠으며 노출된 각화세포의 세포질 표면은 약간의 주름이 형성되어 있었다. 30% 글리콜산으로 처리한 mice 피부에서 각질층 표면은 매끄러웠으며 이들 표면은 주름이 형성되어 있지 않았다.

In this study, we applied 15% glycolic acid peeling agent and 30% glycolic acid peeling agent to the dorsal skin of mice and analyzed the oil content, moisture content, and pH value of the skin before and after test using cutometer. Ultrastructure for changes in the epidermis of mice were observed under a dermoscope and a scanning electron microscope before and after test. When using 15% glycolic acid peeling agent, the changes of moisture content was measured 74.67 AU for the normal control group and 70.21 AU for the experimental group, the oil content was 13.49 mg/$cm^2$ for the normal control group before test and 8.25 mg/$cm^2$ after test, and the pH value was 6.70 and 5.36 before and after test, respectively. When using 30% glycolic acid peeling agent, the moisture content was measured 74.46 AU for the normal control group before test and 53.50 AU for the experimental group after test, the oil content was 13.82mg/$cm^2$ and 5.70 mg/$cm^2$ before and after test, and the pH value was 6.45 and 4.58 before and after test, respectively. As such, it was found that the changes of moisture and oil content on the skin rely on the concentration of peeling agent and the degree of exfoliation of keratin. The surface of stratum corneum of mice with application of 15% glycolic acid peeling agent was relatively smooth and the exposed cellular surface of keratinocyte had some wrinkles. The surface of stratum corneum of mice with application of 30% glycolic acid peeling agent was smooth. No wrinkles were observed under high-resolution scanning electron microscope.

키워드

참고문헌

  1. Bernstein EF, Underhill CB, Lakkakorpi J, Ditre CM, Uitto J, Yu RJ: Citric acid increases viable epidermal thickness and glycosaminoglycan content of sun damaged skin. Dermatol Surg 23 : 689-694, 1997. https://doi.org/10.1016/S1076-0512(97)00188-X
  2. Calens A, Vaillant L, Lecompte P, Berson M, Gall Y, Lotrette G: Does hormonal skin aging exist, a study of the influence of different hormone therapy regimes on the skin of postmenopausal women using non-invasive measurement techniques. Dermatology 193 : 289-294, 1996. https://doi.org/10.1159/000246272
  3. Choi EY, Chang BS: Morphological changes of human epidermis by glycolic acid peeling. J Cosmetol Sci 16(3) : 623-629, 2010.
  4. Ditre CM, Griffin TD, Murphy GF, Sueki H, Telegan B, Johnson WC: Effects of alpha-hydorxy acids on photoaged skin: a pilot clinical, histologic, and ultrastructural study. J Am Acad Dermatol 34 : 187-195, 1996. https://doi.org/10.1016/S0190-9622(96)80110-1
  5. Ghersetich I, Teofoll P, Gantcheva M, Ribuffo M, Puddu P: Chemical peeling: How, when, why? J Eur Acad Dermatol and Venereol 8 : 1-11, 1997. https://doi.org/10.1016/S0926-9959(96)00107-9
  6. Godin DA, Graham HD: Chemical peels. J Large State Med Soc 150 : 513-519, 1998.
  7. Horikoshi T, Matsumoto M, Usuki A, Igarashi S, Hikima R, Uchiwa H: Effects of glycolic acid on desquamation-regulating proteinases in human stratum corneum. Exp Dermatol 14 : 34-40, 2005. https://doi.org/10.1111/j.0906-6705.2005.00224.x
  8. Hussein MR, Ab-Dief EE, Motaleb AA, Zedan H, Meguid AM: Chemical peeling and microdermabrasion of the skin: Comparative immunohistological and ultrastructural studies. J Dermatol Sci 52(3) : 205-209, 2008. https://doi.org/10.1016/j.jdermsci.2008.07.004
  9. Kim HJ, Seo YJ, Lee WJ, Lee JH, Park JK: Combination treatment of laser and chemical peel on wrinkles. J Korean Dermatol Associ 38(1) : 120-122, 2000.
  10. Kitzmiller WJ, Visscher MO, Maclennan S, Butterfield JL, Fuhrman E, Doughty D, Dawes N: Comparison of a series of superficial chemical peels with a single midlevel chemical peel for the correction of facial actinic damage. Esthetic Surgery J 23(5) : 339-344, 2003. https://doi.org/10.1016/S1090-820X(03)00201-2
  11. Loden M, Andersson AC, Lindberg M: Improvement in skin barrier function in patients with atopic dermatitis after treatment with a moisturizing cream. Br J Dermatol 140 : 264-267, 1999. https://doi.org/10.1046/j.1365-2133.1999.02660.x
  12. Monheit GD, Chastain MA: Chemical peels. Facial Plast Surg Clin North Am 9 : 239-255, 2001.
  13. Okano Y, Abe Y, Masaki H, Santhanam U, Ichihashi M, Funasaka Y: Biological effects of glycolic acid on dermal matrix metabolism mediated by dermal fibroblasts and epidermal keratinocytes. Exp Dermatol 12 : 57-63, 2003. https://doi.org/10.1034/j.1600-0625.12.s2.9.x
  14. Smith WP: Epidermal and dermal effects of topical lactic acid. J Am Acad Dermatol 35 : 388-391, 1996. https://doi.org/10.1016/S0190-9622(96)90602-7
  15. Ueda S, Mitsugi K, Ichige K, Yoshida K, Sakuma T, Ninomiya S, Sudou T: New formulation of chemical peeling agent: 30% salicylic acid in polyethylene glycol absorption and distribution of $^{14}C$-salicylic acid in polyethylene glycol applied topically to skin of hairless mice. J Dermatol Sci 28 : 211-218, 2002. https://doi.org/10.1016/S0923-1811(01)00168-2
  16. Van Scott EJ, Yu RJ: Control of keratinization with alpha-hydroxy acids and related compounds I: Topical treatment of ichthyotic disorders. Arch Dermatol 110 : 586-590, 1974. https://doi.org/10.1001/archderm.110.4.586