한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제27권6호
  • /
  • Pages.652-662
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  • 2010
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

개에서 넙다리뼈 겉질 결손부에 대한 Calcium Sulfate, Demineralized Bone Matrix, Calcium Metaphosphate의 뼈 재생 효과 비교

Osteogenetic Effects of Calcium Sulfate, Demineralized Bone Matrix, and Calcium Metaphosphate in a Canine Femur with Unicortical Defects

  • 최장윤 (경북대학교 수의과대학 수의외과학교실) ;
  • 박세일 (경북대학교 수의과대학 수의외과학교실) ;
  • 권영삼 (경북대학교 수의과대학 수의외과학교실) ;
  • 장광호 (경북대학교 수의과대학 수의외과학교실)
  • Choi, Jang-Yoon (College of Veterinary Medicine, Kyungpook National University) ;
  • Park, Se-Il (College of Veterinary Medicine, Kyungpook National University) ;
  • Kwon, Young-Sam (College of Veterinary Medicine, Kyungpook National University) ;
  • Jang, Kwang-Ho (College of Veterinary Medicine, Kyungpook National University)
  • 심사 : 2010.10.11
  • 발행 : 2010.12.31
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초록

개에서 넙다리뼈 뼈겉질에 결손을 유발하여 뼈 이식재로 사용하는 calcium sulfate (CS), demineralized bone matrix (DBM), calcium metaphosphate (CMP)를 이식하여 뼈 재생 효과를 비교 분석하였다. 비글견 9두, 총 18개의 넙다리뼈에 각각 4개의 결손부를 외과적으로 유발하고, 이 중 3 곳에는 CS, DBM, CMP를 각각 이식하고 나머지 하나는 대조군으로서 총 4개의 군으로 나누어 관찰하였다. 방사선 검사는 수술 전, 수술 후 4, 8, 24주에 실시하였으며, 조직 검사는 4, 8, 24주에 안락사 후에 실시하였다. 방사선 검사에서는 시간 경과에 따른 이식재의 분해성을 관찰할 수 있었으며 뼈겉질의 치유가 이루어짐을 확인하였다. CS군은 빠르게 흡수되어 뼈겉질을 형성하였으며, DBM군은 이식재 주위에 뼈가 형성되면서 점차적으로 겉질뼈를 채우는 양상을 보였다. CMP군은 빠르게 흡수되지 않고 24주가 지난 후에도 이식재 잔여물이 남아 뼈 겉질의 구조적 역할을 수행하면서 뼈를 형성하였다. 방사선 intensity는 대조군이 모든 실험군에 비해서 유의성 있게 낮았다 (p < 0.05). 조직학적 검사에서 CS,DBM,CMP 모두 대조군과 비교할 때 뼈 재생을 촉진시켰으며 (p < 0.05), 뼈 겉질 결손 시 입자 공간이 다소 넓은 DBM보다 CMP가, CMP보다 CS가 뼈 접합체로 적합하였다. CS, DBM, CMP 모두 상당 양의 뼈를 형성하여, 뼈 재생 촉진에 효과적인 것으로 판단되었다.

The purpose of this experiment was to study the effects of demineralized bone matrix (DBM), calcium sulfate (CS), and calcium metaphosphate (CMP) on osteogenesis of unicortical 5-mm-diameter defects in canine femurs. Seventy-two femoral unicortical defects of nine adult beagles (eighteen femurs, four unicortical femoral defects were made in each femur) were made. Three bone graft substitutive materials such as CS, DBM, and CMP and the empty controls were compared each other. The postimplanted specimens were harvested at week 4, 8, and 24 for radiographic, biochemical and histomorphologic evaluation. In radiograph, CS group appeared to be absorbed rapidly and made new cortical bone. Defects of cortical bone was gradually filled with new bone around bone graft materials in DBM group. Bone graft substitutes weren't absorbed rapidly but, remained performing structural roles in cortical bone after 24 weeks in CMP group. Radiographic intensity of control group showed significantly (p < 0.05) lower compared to that of experimental group. Defects treated with either CS, DBM or CMP had more bone formation than the untreated defects (p < 0.05). The results of analysis in the cortical bone region were deduced the conclusions as follows. Three bone graft materials seemed to accelerate the formation of new bone compared with controls for 24 weeks. CMP group having more or less large particle space was more adequate than DBM group, as well as more compact CS group was more pertinent than CMP group as the glues for bones.

키워드

참고문헌

  1. Baksh D, Davies JE, Kim S. Three-dimensional matrics of calcium polyphosphates support bone growth in vitro and in vivo. J Mater Sci Mater Med 1998; 9: 743-748.
  2. Behravesh E, Yasko AW, Engel PS, Mikos AG. Synthetic biodegradable polymers for orthopaedic applications. Clin Orthop Relat Res 1999; 367: 118-129. https://doi.org/10.1097/00003086-199910001-00012
  3. Chesmel KD, Branger J, Wertheim H, Scarborough N. Healing response to various forms of human demineralized bone matrix in athymic rat cranial defects. J Oral Maxillofac Surg 1998; 56: 857-865. https://doi.org/10.1016/S0278-2391(98)90015-5
  4. Cobos JA, Lindsey RW, Gugala Z. The cylindrical titanium mesh cage for treatment of a long bone segmental defect: description of a new technique and report of two cases. J Orthop Trauma 2000; 14: 54-59. https://doi.org/10.1097/00005131-200001000-00011
  5. de Boer HH. The history of bone grafts. Clin Orthop Relat Res 1988; 226: 292-298.
  6. Edwards JT, Diegmann MH, Scarborough NL. Osteoinduction of human demineralized bone: characterization in a rat model. Clin Orthop Relat Res 1998; 357: 219-228. https://doi.org/10.1097/00003086-199812000-00028
  7. Friedlaender GE, Perry CR, Cole JD, Cook SD, Cierny G, Muschler GF, Zych GA, Calhoun JH, LaForte AJ, Yin S. Osteogenic protein-1 (bone morphogenetic protein-7) in the treatment of tibial nonunions. J Bone Joint Surg Am 2001; 83: 151-158.
  8. Glancy GL, Brugioni DJ, Eilert RE, Chang FM. Autograft versus allograft for benign lesions in children. Clin Orthop Relat Res 1991; 262: 28-33.
  9. Gupta R, Pandit N, Malik R, Sood S. Clinical and radiological evaluation of an osseous xenograft for the treatment of infrabony defects. J Can Dent Assoc 2007; 73: 513.
  10. Hing KA, Wilson LF, Buckland T. Comparative performance of three ceramic bone graft substitutes. Spine J 2007; 7: 475-490. https://doi.org/10.1016/j.spinee.2006.07.017
  11. Huan Z, Chang J. Self-setting properties and in vitro bioactivity of calcium sulfate hemihydrate-tricalcium silicate composite bone cements. Acta Biomater 2007; 3: 952-960. https://doi.org/10.1016/j.actbio.2007.05.003
  12. Johnson EE, Urist MR, Finerman GA. Resistant nonunions and partial or complete segmental defects of long bones. Treatment with implants of a composite of human bone morphogenetic protein (BMP) and autolyzed, antigen-extracted, allogeneic (AAA) bone. Clin Orthop Relat Res 1992; 277: 229-237.
  13. Jung Y, Kim SS, Kim YH, Kim SH, Kim BS, Kim S, Choi CY, Kim SH. A poly (lactic acid)/calcium metaphosphate composite for bone tissue engineering. Biomaterials 2005; 26: 6314-6322. https://doi.org/10.1016/j.biomaterials.2005.04.007
  14. Kelly CM, Wilkins RM, Gitelis S, Hartjen C, Watson JT, Kim PT. The use of a surgical grade calcium sulfate as a bone graft substitute: results of a multicenter trial. Clin Orthop Relat Res 2001; 382: 42-50. https://doi.org/10.1097/00003086-200101000-00008
  15. Kim SS, Ahn KM, Park MS, Lee JH, Choi CY, Kim BS. A poly (lactide-co-glycolide) / hydroxyapatite composite scaffold with enhanced osteoconductivity. J Biomed Mater Res A 2007; 80: 206-215.
  16. Lee YM, Seol YJ, Lim YT, Kim S, Han SB, Rhyu IC, Baek SH, Heo SJ, Choi JY, Klokkevold PR, Chung CP. Tissue-engineered growth of bone by marrow cell transplantation using porous calcium metaphosphate matrices. J Biomed Mater Res 2001; 54: 216-223. https://doi.org/10.1002/1097-4636(200102)54:2<216::AID-JBM8>3.0.CO;2-C
  17. Leupold JA, Barfield WR, An YH, Hartsock LA. A comparison of ProOsteon, DBX, and collagraft in a rabbit model. J Biomed Mater Res B Appl Biomater 2006; 79: 292-297.
  18. Martin GJ Jr, Boden SD, Titus L, Scarborough NL. New formulations of demineralized bone matrix as a more effective graft alternative in experimental posterolateral lumbar spine arthrodesis. Spine 1999; 24: 637-645. https://doi.org/10.1097/00007632-199904010-00005
  19. Meinel L, Betz O, Fajardo R, Hofmann S, Nazarian A, Cory E, Hilbe M, McCool J, Langer R, Vunjak-Novakovic G, Merkle HP, Rechenberg B, Kaplan DL, Kirker-Head C. Silk based biomaterials to heal critical sized femur defects. Bone 2006; 39: 922-931. https://doi.org/10.1016/j.bone.2006.04.019
  20. Mirzayan R, Panossian V, Avedian R, Forrester DM, Menendez LR. The use of calcium sulfate in the treatment of benign bone lesions. A preliminary report. J Bone Joint Surg Am 2001; 83: 355-358. https://doi.org/10.1302/0301-620X.83B3.11288
  21. Morone MA, Boden SD. Experimental posterolateral lumbar spinal fusion with a demineralized bone matrix gel. Spine 1998; 23: 159-167. https://doi.org/10.1097/00007632-199801150-00003
  22. Nyan M, Sato D, Oda M, Machida T, Kobayashi H, Nakamura T, Kasugai S. Bone formation with the combination of simvastatin and calcium sulfate in critical-sized rat calvarial defect. J Pharmacol Sci 2007; 104: 384-386. https://doi.org/10.1254/jphs.SC0070184
  23. Park EK, Lee YE, Choi JY, Oh SH, Shin HI, Kim KH, Kim SY, Kim S. Cellular biocompatibility and stimulatory effects of calcium metaphosphate on osteoblastic differentiation of human bone marrow-derived stromal cells. Biomaterials 2004; 25: 3403-3411. https://doi.org/10.1016/j.biomaterials.2003.10.031
  24. Parikh SN. Bone graft substitutes: past, present, future. J Postgrad Med 2002; 48: 142-148.
  25. Peltier LF. The use of plaster of Paris to fill large defects in bone: a preliminary report. 1959. Clin Orthop Relat Res 2001; 382: 3-5. https://doi.org/10.1097/00003086-200101000-00002
  26. Peltier LF, Jones RH. Treatment of unicameral bone cysts by curettage and packing with plaster-of-Paris pellets. J Bone Joint Surg Am 1978; 60: 820-822.
  27. Reynolds MA, Aichelmann-Reidy ME, Kassolis JD, Prasad HS, Rohrer MD. Calcium sulfate-carboxymethylcellulose bone graft binder: Histologic and morphometric evaluation in a critical size defect. J Biomed Mater Res B Appl Biomater 2007; 83: 451-458.
  28. Salgado AJ, Coutinho OP, Reis RL. Bone tissue engineering: State of the art and future trends. Macromol Biosci 2004; 9: 4: 743-765.
  29. Schindler OS, Cannon SR, Briggs TW. Use of a novel bone graft substitute in peri-articular bone tumours of the knee. Knee 2007; 5: 1-7.
  30. Wang JC, Alanay A, Mark D, Kanim LE, Campbell PA, Dawson EG, Lieberman JR. A comparison of commercially available demineralized bone matrix for spinal fusion. Eur Spine J 2007; 16: 1233-1240. https://doi.org/10.1007/s00586-006-0282-x
  31. Wang T, Dang G, Guo Z, Yang M. Evaluation of autologous bone marrow mesenchymal stem cell-calcium phosphate ceramic composite for lumbar fusion in rhesus monkey interbody fusion model. Tissue Eng 2005; 11: 1159-1167. https://doi.org/10.1089/ten.2005.11.1159
  32. Yuan H, van Blitterswijk CA, de Groot K, de Bruijn JD. A comparison of bone formation in biphasic calcium phosphate (BCP) and hydroxyapatite (HA) implanted in muscle and bone of dogs at different time periods. J Biomed Mater Res A 2006; 78: 139-147.
  33. Yuan H, Van Den Doel M, Li S, Van Blitterswijk CA, De Groot K, De Bruijn JD. A comparison of the osteoinductive potential of two calcium phosphate ceramics implanted intramuscularly in goats. J Mater Sci Mater Med 2002; 13: 1271-1275. https://doi.org/10.1023/A:1021191432366