Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Osteogenetic Effects of Calcium Sulfate, Demineralized Bone Matrix, and Calcium Metaphosphate in a Canine Femur with Unicortical Defects

개에서 넙다리뼈 겉질 결손부에 대한 Calcium Sulfate, Demineralized Bone Matrix, Calcium Metaphosphate의 뼈 재생 효과 비교

  • Choi, Jang-Yoon (College of Veterinary Medicine, Kyungpook National University) ;
  • Park, Se-Il (College of Veterinary Medicine, Kyungpook National University) ;
  • Kwon, Young-Sam (College of Veterinary Medicine, Kyungpook National University) ;
  • Jang, Kwang-Ho (College of Veterinary Medicine, Kyungpook National University)
  • 최장윤 (경북대학교 수의과대학 수의외과학교실) ;
  • 박세일 (경북대학교 수의과대학 수의외과학교실) ;
  • 권영삼 (경북대학교 수의과대학 수의외과학교실) ;
  • 장광호 (경북대학교 수의과대학 수의외과학교실)
  • Accepted : 2010.10.11
  • Published : 2010.12.31
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Abstract

The purpose of this experiment was to study the effects of demineralized bone matrix (DBM), calcium sulfate (CS), and calcium metaphosphate (CMP) on osteogenesis of unicortical 5-mm-diameter defects in canine femurs. Seventy-two femoral unicortical defects of nine adult beagles (eighteen femurs, four unicortical femoral defects were made in each femur) were made. Three bone graft substitutive materials such as CS, DBM, and CMP and the empty controls were compared each other. The postimplanted specimens were harvested at week 4, 8, and 24 for radiographic, biochemical and histomorphologic evaluation. In radiograph, CS group appeared to be absorbed rapidly and made new cortical bone. Defects of cortical bone was gradually filled with new bone around bone graft materials in DBM group. Bone graft substitutes weren't absorbed rapidly but, remained performing structural roles in cortical bone after 24 weeks in CMP group. Radiographic intensity of control group showed significantly (p < 0.05) lower compared to that of experimental group. Defects treated with either CS, DBM or CMP had more bone formation than the untreated defects (p < 0.05). The results of analysis in the cortical bone region were deduced the conclusions as follows. Three bone graft materials seemed to accelerate the formation of new bone compared with controls for 24 weeks. CMP group having more or less large particle space was more adequate than DBM group, as well as more compact CS group was more pertinent than CMP group as the glues for bones.

개에서 넙다리뼈 뼈겉질에 결손을 유발하여 뼈 이식재로 사용하는 calcium sulfate (CS), demineralized bone matrix (DBM), calcium metaphosphate (CMP)를 이식하여 뼈 재생 효과를 비교 분석하였다. 비글견 9두, 총 18개의 넙다리뼈에 각각 4개의 결손부를 외과적으로 유발하고, 이 중 3 곳에는 CS, DBM, CMP를 각각 이식하고 나머지 하나는 대조군으로서 총 4개의 군으로 나누어 관찰하였다. 방사선 검사는 수술 전, 수술 후 4, 8, 24주에 실시하였으며, 조직 검사는 4, 8, 24주에 안락사 후에 실시하였다. 방사선 검사에서는 시간 경과에 따른 이식재의 분해성을 관찰할 수 있었으며 뼈겉질의 치유가 이루어짐을 확인하였다. CS군은 빠르게 흡수되어 뼈겉질을 형성하였으며, DBM군은 이식재 주위에 뼈가 형성되면서 점차적으로 겉질뼈를 채우는 양상을 보였다. CMP군은 빠르게 흡수되지 않고 24주가 지난 후에도 이식재 잔여물이 남아 뼈 겉질의 구조적 역할을 수행하면서 뼈를 형성하였다. 방사선 intensity는 대조군이 모든 실험군에 비해서 유의성 있게 낮았다 (p < 0.05). 조직학적 검사에서 CS,DBM,CMP 모두 대조군과 비교할 때 뼈 재생을 촉진시켰으며 (p < 0.05), 뼈 겉질 결손 시 입자 공간이 다소 넓은 DBM보다 CMP가, CMP보다 CS가 뼈 접합체로 적합하였다. CS, DBM, CMP 모두 상당 양의 뼈를 형성하여, 뼈 재생 촉진에 효과적인 것으로 판단되었다.

Keywords

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