Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Anaylsis of ${\gamma}$-aminobutyric Acid (GABA) Content in Germinated Pigmented Rice

발아 유색미의 GABA(${\gamma}$-aminobutyric acid) 함량 분석

  • An, Mi-Kyoung (Department of Food Science and Biotechnology, Dongguk University-Seoul) ;
  • Ahn, Jun-Bae (Department of Food Service Industry, Seowon University-Chungju) ;
  • Lee, Sang-Hwa (Department of Food and Nutrition, Seowon University-Chungju) ;
  • Lee, Kwang-Geun (Department of Food Science and Biotechnology, Dongguk University-Seoul)
  • 안미경 (동국대학교 식품생명공학과) ;
  • 안준배 (서원대학교 외식산업학과) ;
  • 이상화 (서원대학교 식품영양학과) ;
  • 이광근 (동국대학교 식품생명공학과)
  • Received : 2010.06.14
  • Accepted : 2010.08.11
  • Published : 2010.10.31
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Abstract

The level of ${\gamma}$-aminobutyric acid (GABA) in pigmented rice was quantitatively and qualitatively determined by high pressure liquid chromatography/fluorescence detection (HPLC/FLD). In this study, the recovery rate and limit of detection (LOD) of GABA were 122.4${\pm}$2.4% and 0.23 ${\mu}g/g$, respectively. The geminating pigmented rice samples, which were harvested in Paju, Korea, were soaked in water at $18^{\circ}C$ for 20 hr. After soaking, the samples were germinated at $30^{\circ}C$ for about 24 hr. GABA content was highest (293.0 ${\mu}g/g$) in the germinated red rice. Furthermore, GABA levels in the germinated rice increased significantly by up to 11.1 and 24.7-fold as compared to non-germinated rice and milled rice, respectively. The GABA concentrations of non-germinated rice, with the exception of red rice, were significantly higher than those of milled rice by 7.6-20.6-fold.

본 연구는 현미, 녹미, 홍미, 흑미 4종의 유색미의 발아에 따른 GABA 함량을 분석하고 백미와 함께 비교하였다. HPLC/FLD를 이용하여 GABA의 정성 및 정량 분석을 실시하였으며, 회수율과 검출한계를 설정하였다. 회수율을 측정한 결과 122.4${\pm}$2.4%, RSD는 2.0%이었으며, 검출한계는 0.23 ${\mu}g/g$, 정량한계는 0.76 ${\mu}g/g$이었다. 시료는 $18^{\circ}C$에서 20시간 침지시킨 후, 30에서 20-24시간 동안 발아시켰다. 발아미, 미발아미 및 백미의 GABA 함량을 분석한 결과, 발아 홍미가 293.6 ${\mu}g/g$으로 유의적으로 가장 많은 양을 함유하고 있었으며 홍미와 백미가 각각 26.4, 11.9 ${\mu}g/g$으로 가장 적은 양을 함유하고 있었다. 발아미와 미발아미의 GABA 함량을 비교한 결과, 녹미를 제외한 현미, 홍미, 흑미는 미발아미에 비해 GABA의 함량이 유의적으로 2.5-11.1배 증가 하였다. 백미와 미발아미를 비교해 본 결과, 백미와 홍미 사이에 유의적 차이를 보이지는 않았으며, 현미, 녹미와 흑미는 백미의 GABA 함량인 11.9 ${\mu}g/g$에 비해 7.6-20.6배 높은 함량이었다. 백미와 발아미 사이에는 발아미는 모두 백미와 유의적인 차이를 보였으며 백미에 비해 발아미의 GABA 함량은 19.0-24.7배 높았다.

Keywords

References

  1. Chung HS, Shin JC. Characterization of antioxidant alkaloids and phenolic acids from anthocyanin-pigmented rice (Oryza sativa cv. Heugjinjubyeo). Food Chem. 104: 1670-1677 (2007) https://doi.org/10.1016/j.foodchem.2007.03.020
  2. Tran TU, Suzuki K, Okadome H, Homma S, Ohtsubo K. Analysis of the tastes of brown rice and milled rice with different milling yields using a taste sensing system. Food Chem. 88: 557-566 (2004) https://doi.org/10.1016/j.foodchem.2004.02.007
  3. Woo SM, Kim TY, Yeo SH, Kim SB, Kim JS, Kim MH, Jeong YJ. Quality characteristics of alcohol fermentation broth and byproduct of brown rice varieties. 14: 557-563 (2007)
  4. Kunlun L, Xiaohong C, Qingyun B, Huanbin W, Zhenxin G. Relationships between physical properties of brown rice and degree of milling and loss of selenium. J. Food Eng. 94: 69-74 (2009) https://doi.org/10.1016/j.jfoodeng.2009.03.001
  5. Kenichi O, Keitaro S, Yuji Y, Takafumi K. Bio-functional components in the processed pre-germinated brown rice by a twin-screw extruder. J. Food Compos. Anal. 18: 303-316 (2005) https://doi.org/10.1016/j.jfca.2004.10.003
  6. Ha TY, Park SH, Lee CH, Lee SH. Chemical composition of pigmented rice varieties. Korean J. Food Sci. Technol. 31: 336-341 (1999)
  7. Kang SY, Shin IC, Kim DS, Lee GJ, Kim JB, Lee DY, Lee SY, Lee DJ. A new green-kerneled glutinous rice mutant variety, 'Nogwonchalbyeo' developed by $\gamma$-ray irradiation. Korean J. Breed Sci. 40: 303-307 (2008)
  8. Lee YS, Cho JM, Rhee CO. Physicochemical properties of pigmented rice (Suwon-415). Korean J. Food Sci. Technol. 34: 842-845 (2002)
  9. Jeon HY, Kim JK, Seo DB, Lee SJ. Effects of chlorophyll a on UVB-induced cellular resposes and type pN collagen synthesis in vitro. Korean J. Food Sci. Technol. 41: 700-705 (2009)
  10. Choi YM, Jeon GU, Kong SH, Lee JS. Changes in GABA content of selected specialty rice after germination. Food Eng. Progr. 13: 154-158 (2009)
  11. Choi HD, Kim YS, Choi IW, Seog HM, Park YD. Anti-obesity and cholesterol-lowering effects of germinated brown rice in rats fed with high fat and cholesterol diets. Korean J. Food Sci. Technol. 38: 674-678 (2006)
  12. Choi HD, Park YK, Kim YS, Chung CH, Park YD. Effect of pretreatment conditions on $\gamma$-aminobutyric acid content of brown rice and germinated brown rice. Korean J. Food Technol. 36: 761-764 (2004)
  13. Oh SH, Choi WG. Production of the quality germinated brown rices containg high $\gamma$-aminobutyric acid by chitosan application. Korean J. Biotechnol. Bioeng. 15: 615-620 (2000)
  14. Oh SH, Kim SH, Moon YJ, Choi WG. Changes in the levels of ${\gamma}$-aminobutyric acid and some amino acids by application of a glutamic acid solution for the germination of brown rices. Korean J. Biotechnol. Bioeng. 17: 49-53 (2002)
  15. Xing SG, Jun YB, Hau ZW, Liang LY. Higher accumulation of $\gamma$- aminobutyric acid induced by salt stress through stimulating the activity of diamine oxidases in Glycine max (L.) Merr. roots. Plant Physiol. Bioch. 45: 560-566 (2007) https://doi.org/10.1016/j.plaphy.2007.05.007
  16. Chung HJ, Jang SH, Cho HY, Lim ST. Effects of steeping and anaerobic treatment on GABA ($\gamma$-aminobutyric acid) content in germination waxy hull-less barely. LWT-Food Sci. Technol. 42: 1712-1716 (2009) https://doi.org/10.1016/j.lwt.2009.04.007
  17. Wang HF, Tsai YS, Lin ML, Ou AS. Comparison of bioactive components in GABA tea and green tea produced in Taiwan. Food Chem. 96: 648-653 (2006) https://doi.org/10.1016/j.foodchem.2005.02.046
  18. Qingyun B, Meiqing, Zhenxin G, Xiaohong C, Yan L, Kunlun L. Effects of components in culuture medium on glutamate decarboxylase activity and $\gamma$-amino butyric acid accumulation in foxtail millet (Setaria italic L.) during germination. Food Chem. 116: 152-157 (2009) https://doi.org/10.1016/j.foodchem.2009.02.022
  19. Zhang H, Yao HY, Chen F. Accumulation of $\gamma$-amino butyric acid in rice germ using protease. Biosci. Biotech. Bioch. 70: 1160-1165 (2006) https://doi.org/10.1271/bbb.70.1160
  20. Bjork JM, Moeller FG, Kramer GL, Kram M, Suris A, Rush AJ, Petty F. Plasma GABA levels correlate with aggressiveness in relatives of patients with unipolar depressive disorder. Psychiat. Res. 101: 131-136 (2001) https://doi.org/10.1016/S0165-1781(01)00220-7
  21. Iimure T, Kihara M, Hirota N, Zhou T, Hayashi K, Ito K. A method for production of c-amino butyric acid (GABA) using barley bran supplemented with glutamate. Food Res. Int. 42: 319-323 (2009) https://doi.org/10.1016/j.foodres.2008.12.010
  22. Oh SH. Stimulation of $\gamma$-aminobutyric acid synthesis activity in brown rice by a chotosan/glutamic acid germination solution and calcium/calmodulin. J. Biochem. Mol. Biol. 36: 319-325 (2003) https://doi.org/10.5483/BMBRep.2003.36.3.319
  23. Komatsuzaki N, Tsukahara K, Toyoshima H, Suzuki T, Shimizu N, Kimura T. Effect of soaking and gaseous treatment on GABA content in germinated brown rice. J. Food Eng. 78: 556-560 (2007) https://doi.org/10.1016/j.jfoodeng.2005.10.036
  24. Nam SH, Choi SP, Kang MY, Koh HJ, Kozukue N, Friedman M. Antioxidative activities of bran extracts from twenty one pigmented rice cultivars. Food Chem. 94: 613-620 (2006) https://doi.org/10.1016/j.foodchem.2004.12.010
  25. Shen Y, Jin L, Xiao P, Lu Y, Bao J. Total phenolics, flavonoids, antioxidant capacity in rice grain and their relations to grain color, size, and weight. J. Cereal Sci. 49: 106-111 (2009) https://doi.org/10.1016/j.jcs.2008.07.010
  26. Harris DC. Exploring chemical analysis. 3rd edition. W. H. Freeman, New York, NY, USA pp. 91-98 (2001)
  27. Eckstein JA, Ammerman GM, Reveles JM, Ackermann BL. Analysis of glutamine, glutamate, pyroglutamate, and GABA in cerebrospinal fluid using ion pairing HPLC with positive electrospray LC/MS/MS. J. Neurosci. Meth. 171:190-196 (2008) https://doi.org/10.1016/j.jneumeth.2008.02.019
  28. Choi ID, Kim DS, Son JG, Yang CG, Chun JY, Kim KJ. Physicochemical properties of giant embryo brown rice (Keunnunbyeo). Agric. Chem. Biotechnol. 49: 95-100 (2006)
  29. Kum JS, Choi BK, Lee HY, Park JD. Physicochemical properties of germinated brown rice. Korean J. Food Preserv. 11: 182-188 (2004)
  30. Matsuyama A, Yoshimura K, Shimizu C, Murano Y, Takeuchi H, Ishimoto M. Characterization of glutamate decarboxylase mediating $\gamma$-amino butyric acid increase in the early germination stage of soybean (Glycine max [L.] Merr). J. Biosci. Bioeng. 107: 538-543 (2009) https://doi.org/10.1016/j.jbiosc.2009.01.012