한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

축산식품 중 벤지미다졸계 구충제 잔류실태 조사

Determination of Benzimidazole Residues in Livestock Products

  • 이경진 (경인지방식품의약품안전청 수입식품분석과) ;
  • 강영운 (식품의약품안전청 오염물질과) ;
  • 강은귀 (경인지방식품의약품안전청 수입식품분석과) ;
  • 김미란 (경인지방식품의약품안전청 수입식품분석과) ;
  • 반경녀 (경인지방식품의약품안전청 수입식품분석과) ;
  • 장영미 (부산지방식품의약품안전청 수입식품분석과) ;
  • 김미혜 (식품의약품안전청 오염물질과)
  • Lee, Kyung-Jin (Imported Food Analysis Division Center for Food and Drug Analysis, Gyeongin Regional KFDA) ;
  • Kang, Young-Woon (Food Contaminant Division, Food Safety Evaluation Department, KFDA) ;
  • Kang, Eung-Ui (Imported Food Analysis Division Center for Food and Drug Analysis, Gyeongin Regional KFDA) ;
  • Kim, Mi-Ran (Imported Food Analysis Division Center for Food and Drug Analysis, Gyeongin Regional KFDA) ;
  • Bahn, Kyeong-Nyeo (Imported Food Analysis Division Center for Food and Drug Analysis, Gyeongin Regional KFDA) ;
  • Jang, Young-Mi (Imported Food Analysis Division Center for Food and Drug Analysis, Busan Regional KFDA) ;
  • Kim, Mee-Hye (Food Contaminant Division, Food Safety Evaluation Department, KFDA)
  • 투고 : 2009.11.26
  • 심사 : 2010.03.12
  • 발행 : 2010.10.31
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초록

본 연구를 통하여 확립된 시험법은 고감도를 가지고 선택성이 뛰어난 LC/MS/MS를 이용하여 한번의 시료전처리와 분석을 통하여 축산식품 중 잔류하는 벤지미다졸계 구충제인 albendazole, fenbendazole, thiabendazole, flubendazole 및 oxibendazole 5종의 잔류량 분석을 가능하게 하였다. 추출용매로 ethyl acetate를 사용하고 MCX 카트리지를 이용하여 정제를 한 시험법으로 Codex guideline(20)에 따라 검량선의 직선성, 회수율, 검출한계 및 정량 한계 등에 대한 실효성을 검증한 결과 검량선은 모두 0.999 이상의 좋은 직선성을 가졌으며 회수율은 albendazole 71-74%, fenbendazole 70-72%, thiabendazole 79-83%, flubendazole 79-83%, oxibendazole 81-86%로 좋은 결과를 얻었다. 또한 검출한계 및 정량한계는 0.01 ppb, 0.03 ppb로 잔류허용기준이 가장 낮은 flubendazole의 10 ppb보다 100배 정도 더 좋은 감도를 가지고 있으며, 소의 근육, 간, 및 천엽에서 MSPD법으로 분석한 실험(21)과 정제과정 없이 유기용매로 추출하여 LC/MS로 분석한 실험의 회수율(22)보다 본 연구의 시험법이 더 좋은 회수율을 보여주었다. 또한 확립된 시험법을 이용하여 유통 중인 돼지고기, 쇠고기, 닭고기 총 270건을 수거하여 잔류량을 모니터링 한 결과 경기, 충청, 전라, 경상 지역에서 수거한 돼지고기에서 fenbendazole이 0.0012-0.0031 mg/kg의 수치로 검출이 되었고, 그 검출률은 4.4%이었으나 잔류허용기준인 0.1 mg/kg보다 낮은 수준이었다. 또한 fenbendazole에 대한 노출량 평가 결과 JECFA에서 정하고 있는 ADI의 0.01% 이하로 나타났다. 그러나 축산 농가에서 축산물의 생산량을 높이기 위하여 벤지미다졸계 구충제를 과다 사용하거나 안전 휴약기간을 준수하지 않을 경우 축산물의 체내에 잔류가능성이 높기 때문에 지속적인 잔류실태 조사가 수행되어야 할 것으로 사료된다.

This research investigated benzimidazole residues (albendazole, fenbendazole, flubendazole, thiabendazole, oxibendazole) in livestock products. A total of 270 samples of livestock products (beef, pork and chicken) were purchased from local markets in Korea. Ethyl acetate was used to extract analytes from the sample, after which ethyl acetate extracts were purified using a MCX cartridge. Analytes were detected using liquid chromatography-tandem mass spectrometry. The limit of detection was 0.01-0.04 ppb, the limit of quantification was 0.03-0.13 ppb, the linearity ($r^2$) was 0.9992-1.0000, and the recovery was 70-85%. Residues of benzimidazoles, except for fenbendazole in pork, were not found in any of the 270 livestock samples. Fenbendazole was detected in the range of 1.2 to 3.1 ppb in 12 samples of pork.

키워드

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