한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology)

  • 제23권3호
  • /
  • Pages.33-41
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  • 2010
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  • 1738-6640(pISSN)
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  • 2234-4020(eISSN)
대한한방안이비인후피부과학회 (The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology)
권호 표지

수종(數種) 한약재의 콜라겐 생합성 효과에 대한 실험적 연구

Experimental study about the effect of several herbs on collagen synthesis

  • 윤지은 (원광대학교 한의과대학 안이비인후피부과) ;
  • 김남권 (원광대학교 한의과대학 안이비인후피부과) ;
  • 황충연 (원광대학교 한의과대학 안이비인후피부과) ;
  • 조은희 (원광대학교 한의과대학 침구과) ;
  • 박민철 (원광대학교 한의과대학 안이비인후피부과)
  • 투고 : 2010.11.07
  • 심사 : 2010.12.04
  • 발행 : 2010.12.25
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초록

Background & Objective : This study was performed to find a new anti-aging, anti-wrinkle agent. Ten kinds of hers were evaluated by proliferation, cytotoxicity, collagen synthesis tests in cultured human dermal fibroblast. Methods : MTT assay was carried out to check cell proliferation, cytotoxic effect of each herbal medicine. Collagen synthesis was investigated by the amount of propeptide using procollagen type-Ⅰ C peptide(PIP) ELISA kit(Takara). Results and Conclusions : There are no cytotoxicity in all group herbs by MTT assay. Collagen synthetic ability was greatest in LYCII FRUCTUS(LF) and was excellent in order of Rehmanniae Radix Preparat(RRP) > Nelumbinis Semen(NS) > Asparai Radix(AR) > Schizandrae Frucus(SF).

키워드

참고문헌

  1. Branchet MC, Boisnic S, Frances C et al. Skin thickness changes in normal aging skin. Gerontology. 1990;36:28-35. https://doi.org/10.1159/000213172
  2. Green LJ, McCormic A, Weinstein GD. Photoaging and the skin: The effects of tretinoin. Dermatol Clin. 1993;11:97-105.
  3. Yaar M, Gilchrest BA. Aging versus photoaging: Postulated Mechanisms and effectors. J Invest Dermtol Symp Proc. 1998;3:47-51. https://doi.org/10.1038/jidsp.1998.12
  4. Jerome S. P., Gabrielle L., and Raul F. Idedntification of Collagen Fibrils in scleroderma skin. J. Invest. Dermatol. 1998;90(1):48-54.
  5. 성은진, 김환영, 안민섭, 김훈영, 조은희, 박민철. 주름 치료의 한의학적 고찰. 한방 안이비인후피부과학회지. 2008;21(3):186-7.
  6. Parfitt AM, Simon LS, Villanueva AR, Krane SM.(1987) Procollagen type I carboxy-terminl extension peptide in serum as a marker of collagen biosynthesis in bone. Correlation with iliac formation rates and compasison with total alkaline phosphatase. J. Bone Miner Res. 1987; 2:427-36.
  7. Loosdrecht AA, Nennie E, Ossenkopple GJ, Beelen RH, Langenhuijsen MIM. Cell mediated cytotoxicity against U937 cells by human monocytes and macrophages in a modified colorimetric MTT assay. A methodological study. J Immunol Method. 1991;141:15-22. https://doi.org/10.1016/0022-1759(91)90205-T
  8. Wilhelm KP, Cua AB, Maibach HI. Skin aging. Effect on transepidermal water loss, stratum corneum hydration, skin surface pH, and casual sebum content. Arch Dermatol. 1991;127:1806-9. https://doi.org/10.1001/archderm.127.12.1806
  9. Gilchrest BA. Skin aging and photoaging: an overview. J Am Acad Dermatol. 1989;21:610-3. https://doi.org/10.1016/S0190-9622(89)70227-9
  10. Bernstein EF, Chen YQ, Tamai K, Shepley KJ, Resnik KS, Zhang H, Tuan R, Mauviel A, Uitto J. Enhanced elastin and fibrillin gene expression in chronically photodamaged skin. J Invest Dermatol. 1994;103(2):182-6. https://doi.org/10.1111/1523-1747.ep12392693
  11. Chiba K, Kawakami K, Sone T, Onoue M. Characteristics of skin wrinkling and dermal changes induced by repeated application of squalene monohydroperoxide to hairless mouse skin. Skin Pharmacology and Applied Skin Physiology. 2003;16: 242-51. https://doi.org/10.1159/000070847
  12. 朴贊國. 黃帝內經素問注釋. 集文堂. 2005: 7-11,95-7.
  13. Burgeson RE, Nimni ME. Collagen types : Molecular structure and tissue distribution. Clin Orthop. 1992;282:250-72.
  14. Ihn, H. Scleroderma, fibroblast, signaling and excessive extracellular matrix. Curr Rheumatol. 2005;7:156-62. https://doi.org/10.1007/s11926-005-0069-9
  15. Wulf H. C, Sandby-Moller J, Kobayasi T, Gniadlecki R. Skin aging and natural photoprotection. Micron. 2004;35:85-191.
  16. 대한피부과학회 교과서 편찬위원회. 피부과학. 麗文閣. 1994:17-8.
  17. Giacomoni PU, Rein G. Factors of skin aging share common mechanisms. Biogerontology. 2001;2(4):210-29.
  18. Parfitt AM, Simon LS, Villaneuva AR, Krane SM. Procollagen type I carboxyterminal extention peptide in serum as a marker of collagen biosynthesis in bone. Correlation with iliac bone formation rates and comparison with total alkaline phosphatase. J. Bone Miner Res. 1987; 2:427-36.
  19. Bonte F, Dumas M, Chaudagne C, Meybeck A. Influence of asiatic acid, madecassic acid, and aisiaticoside on human collagen I synthesis. Planta Med. 1994;60(2):133-5. https://doi.org/10.1055/s-2006-959434
  20. 김나미, 구본석, 이성계, 황의일, 소승호, 도재호. 홍삼성분이 섬유아세포의 콜라겐 생합성과 MMP-1 활성에 미치는 영향. 고려인삼학회지. 2007;31(2):86-92.
  21. 정택규, 김미진, 임경란, 윤경섭. 황기추출물의 보습 및 항산화 효과. 대한화장품학회지. 2006;32(3):193-200.
  22. 이태완, 김상년, 지웅길, 황성주. 유백피 추출물을 함유한 하이드로겔 패치의 주름 억제 효과. 약제학회지 2004;34(3):193-9.
  23. 진무현, 정민환, 임영희, 이상화, 강상진, 조완구. 백지의 콜라겐 생성 촉진 물질. 생약학회지. 2004;35(4):315-9.
  24. 구본석, 황의일, 소승호, 이성계, 한경호, 김나미. 사상자가 섬유아세포의 Procollagen 생합성과 MMP-1의 활성에 미치는 영향. 생약학회지. 2007;38(4):349-53.
  25. 강성례, 민경진, 김영철. 삼백초 열수추출물의 피부주름 억제효과. 한국미용학회지. 2009;15 (4):1389-98.
  26. Ukeda H, Maeda S, Ishii T, Sawamura M. Spectrophotometric assay for superoxide dismutase based on tetrazolium salt 3'-1- (phenylamino)-carbonyl-3, 4-tetrazolium-bis (4-methoxy-6-nitro benzensulfonic acid hydrate reduction by xanthine-xanthine oxidase). Anal Biochem. 1997;5:206-10.