생약학회지 (Korean Journal of Pharmacognosy)

  • 제41권4호
  • /
  • Pages.250-254
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  • 2010
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  • 0253-3073(pISSN)
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  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

추출용매에 따른 산초 (Zanthoxylum schnifolium)열매 추출물의 라디칼소거능과 항염증 효과

Radical Scavenging and Anti-inflammation Activities from Different Extracts of Zanthoxylum schnifolium Fruits

  • 한웅 (강원대학교 의생명과학대학 의생명공학부) ;
  • 왕명현 (강원대학교 의생명과학대학 의생명공학부)
  • Han, Woong (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University) ;
  • Wang, Myeong-Hyeon (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University)
  • 투고 : 2010.09.18
  • 심사 : 2010.10.28
  • 발행 : 2011.12.31
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초록

This study was presented to the physiological activities such as metal chelating capacity, superoxide dismutase-like activity, anti-inflammation, cytotoxicity from Zanthoxylum schnifolium fruit. The values for metal chelating capacity of 1 mg/mL of 70% EtOH and 70% MeOH extracts were 68% and 67%, respectively, it was shown that metal chelating capacity in a dose-dependent manner. The 100% EtOH and 100% MeOH extract exhibited strong superoxide dismutase-like activity. The highest SOD-like activity obtained from 100% EtOH was 51% at a concentration of 4 mg/mL. The all extracts of Zanthoxylum schnifolium fruit except $H_2O$ extract significantly decreased the concentration of LPS-induced NO in Raw 264.7 cells. The 100% EtOH, 100% MeOH and 70% EtOH extract represented the highest activity in the anti-inflammation properties in vitro. However, the 100% EtOH and 100% MeOH extracts has cytotoxicity in Raw 264.7 cells and it may affect cell viability. Conclude that 70% EtOH extract was most suitable for anti-inflammation. Our results revealed that the Zanthoxylum schnifolium fruit expected to physiological activities.

키워드

참고문헌

  1. 박춘근 (2002) 현대인을 위한 민간약초. 작물시험장.
  2. Jang, M. J., Woo, M. H., Kim, Y. H., Jun, D. Y. and Rhee, S. J. (2005) Effects of antioxidative, DPPH radical scavenging activity and antithrombogenic by the extract of Sancho (Zanthoxylum schinifolium). Korea J. Nutrition 38: 386-394.
  3. Kim, J. H. (2003) Variation of the leaf monotrpenes concentration of Zanthoxylum schinifolium at Mt. Muhak. J. Basic Sci. 18: 137-149.
  4. Lee, M. S. and Chung, M. S. (2000) Analysis of volatile flavor components from Zanthoxylum schinifolium and sensory evalution as natural spice. Korean J. Soc. Food Sci. 16: 216-220.
  5. Lim, S. J., Han, H. K. and Ko, J. H. (2003) Effects of edible and medicinal plants intake on blood glucose, glycogen and protein levels in streptozotocin induced diabetic rats. Korean J. Nutrition 36: 981-989.
  6. Kim, J. S., Jun, D. Y., Woo, M. H., Rhee, I. K. and Kim Y. H. (2006) Chemical composition and antitumor apoptogenic activity of methylene chloride extracts from the leaves of Zanthoxylum schinifolium. J. life sci. 16: 546-554 https://doi.org/10.5352/JLS.2006.16.3.546
  7. Jang, M., Lee, J. H., Seo, J. and Kim, G. H. (2010) Antibacterial Activities of Essential Oil from Zanthoxylum schinifolium Against Food-Borne Pathogens. Korean J. Food Cookery Sci. 26: 206-213.
  8. Jang, H. S., Rhee, S. J., Woo, M. H. and Cho, S. H. (2007) Anti-thrombogenic and anti-inflammention effect of solvent fraction from leaves of Zanthoxylum schinifolium (Sancho Namu) in rat fed high fat diet. Korean J. Nutrition 40: 606-615.
  9. Chen, I. S., Lin, Y., Tsai, I. L., Teng, C. M., Ko, F. N., Ishikawa, T. and Ishii H. (1995) Coumarins and anti-platelet aggregation constituents from Zanthoxylum schinifolium. Phytochemistry 39: 1091-1097 https://doi.org/10.1016/0031-9422(95)00054-B
  10. Yoon, D. H. and Choi, Y. S. (2008) Influence of red pepper (Capsicum annuum L.) seed oil and sancho(Zanthoxylum schinifolium) seed oil on serum and liver lipids profiles in rats. Korean J. Food Sci. Technol. 40: 96-100.
  11. Kim, S. I. and Han, Y. S. (1997) Isolation and identification of antimicrobial compound from sancho(Zanthoxylum schinifolium). Korean J. Food Sci. Technol. 13: 56-63.
  12. Kim, J. S., Koo, K. M. and Jung Y. H. (2004) Antimicrobial activities of Zanthoxylum schinifolium extract against Vibrio parahaemolyticus. Korean J. Food Sci. Nutr. 33: 500-504. https://doi.org/10.3746/jkfn.2004.33.3.500
  13. Kang-Rotondo, C. H., Major, S., Chiang, T. M., Myers, L. K. and Kang, E. S. (1996) Upregulation of nitric oxide synthase in cultured human keratinocytes after ultraviolet B and bradykinin. Photodermatol Photoimmunol Photomed. 12: 57-65. https://doi.org/10.1111/j.1600-0781.1996.tb00176.x
  14. Seo, S. J., Choi, H. G., Chung, H. J. and Hong, C. K. (2002) Time course of expression of mRNA of inducible nitric oxide synthase and generation of nitric oxide by ultraviolet B in keratinocyte cell lines. Br. J. Dermatol. 147: 655-662. https://doi.org/10.1046/j.1365-2133.2002.04849.x
  15. Weller, R. (1997) Nitric oxideL:a newly discovered chemical transmitter in human skin. Br. J. Dermatol. 137: 665-672. https://doi.org/10.1111/j.1365-2133.1997.tb01099.x
  16. Moncada, S. and Higgs, A. (1993) The L-arginine-nitric oxide pathway. N. Engl. J. Med. 329: 2002-2012. https://doi.org/10.1056/NEJM199312303292706
  17. Shew, R. L., Papka, R. E., McNeill, D. L. and Yee, J. A. (1993) NADPH diaphorase-positive nerves and the role of nitric oxide in CGRP relaxation of uterine contraction. Peptides 14: 637-641. https://doi.org/10.1016/0196-9781(93)90157-C
  18. Kwqamata, H., Ochiai, H., Mantani, N. and Terasawa, K. (2000) Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS activated RAW264.7 cells, a murine macrophage cell line. Am. J. Chin. Med. 28: 217-226. https://doi.org/10.1142/S0192415X0000026X
  19. Lee, B. G., Kim, S. H., Zee, O. P., Lee, K. R., Lee, H. Y., Han, J. W. and Lee, H. W. (2000) Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two-carboline alkaloids extracted from Melia azedarach. Eur. J. Pharmacol. 406: 301-309. https://doi.org/10.1016/S0014-2999(00)00680-4
  20. Seo, W. G., Pae, H. O., Oh, G. S., Chai, K. Y., Yun, Y. G., Kwon, T. O. and Chung, H. T. (2000) Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon and lipopolysaccharide. Gen. Pharmacol. 35: 21-28. https://doi.org/10.1016/S0306-3623(01)00086-6
  21. Chiou, W. F., Chou, C. J. and Chen, C. F. (2001) Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages. Life Sci. 69: 625-635. https://doi.org/10.1016/S0024-3205(01)01154-7
  22. Seo, W. G., Pae, H. O., Oh, G. S., Kim, N. Y., Kwon, T. O., Shin, M. K., Chai, K. Y. and Chung, H. T. (2001) The aqueous extract of Rhodiola sachalinensis root enhances the expression of inducible nitric oxide synthase gene in RAW264.7 macrophage. J. Ethnopharmacol. 76: 119-123. https://doi.org/10.1016/S0378-8741(01)00220-3
  23. Yena, G. C., Duhb, P. D. and Tsai, H. L. (2002) Antioxidant and prooxidant properties of ascorbic acid and gallic acid. Food Chem. 79: 307-313. https://doi.org/10.1016/S0308-8146(02)00145-0
  24. Kim, S. M., Cho, Y. S. and Sung, S. K. (2001) The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J. Food Sci. Technol. 33: 626-632.
  25. Desai, A., Vyas, T. and Amiji, M. (2008) Cytotoxicity and apoptosis enhancement in brain tumor cells upon coadministration of paclitaxel and ceramide in nanoemulsion formulations. J. Pharm. Sci. 97: 2745-275 https://doi.org/10.1002/jps.21182
  26. Li, X. L. and Zhou, A. G. (2007) Evaluation of the antioxidant effects of polysaccharides extracted from Lycium barbarum. Med. Chem. Res. 15: 471-482. https://doi.org/10.1007/s00044-007-9002-2
  27. Kuramoto, T. (1992) Development and application of food materials from extract such as SOD. Up-to-date Food processing. 27: 22-23.
  28. Moncada, S., Palmer, R. M. J. and Higgs, D. A. (1991) Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol. Rev. 43: 109-142.
  29. Southan, G. J. and Szabo, C. (1996) Selective pharmacological inhibition of distinct nitric oxide synthase isoforms. Biochem. Pharmacol. 51: 383-394. https://doi.org/10.1016/0006-2952(95)02099-3
  30. Cao, L. H., Lee, Y. J., Kang, D. G.., Kim, J. S. and Lee, H. S. (2009) Effect of Zanthoxylum schinifolium on TNF-$\alpha$-induced vascular inflammation in human umbilical vein endothelial cell. Vascular pharmacol. 50: 200-207. https://doi.org/10.1016/j.vph.2009.01.008