생명과학회지 (Journal of Life Science)

  • 제20권11호
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  • Pages.1729-1737
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  • 2010
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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가축용 생균제 Lactobacillus plantarum을 이용한 양파즙 발효의 균체성장과 항산화 활성

Cell Growth and Antioxidant Activity on Onion Juice Fermentation by Using Lactobacillus plantarum as Animal Probiotics

  • 장우경 (건국대학교 동물생산환경학) ;
  • 조상범 (건국대학교 동물생산환경학) ;
  • 김동운 (국립축산과학원) ;
  • 이상석 (순천대학교 동물자원과학과) ;
  • 김수기 (건국대학교 동물생산환경학)
  • Chang, Woo-Kyung (Department of Animal Science and Environment, Konkuk University) ;
  • Cho, Sang-Buem (Department of Animal Science and Environment, Konkuk University) ;
  • Kim, Dong-Woon (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Lee, Sang-Suk (Department of Animal Science and Technology, Sunchon University) ;
  • Kim, Soo-Ki (Department of Animal Science and Environment, Konkuk University)
  • 투고 : 2010.11.04
  • 심사 : 2010.11.18
  • 발행 : 2010.11.30
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초록

본 연구는 양파즙을 이용하여 가축 사료첨가제용 Lactobacillus plantarum의 배양 최적화를 위하여 수행되었다. 양파즙의 첨가수준이 균체성장과 항산화 활성에 미치는 영향을 평가하였을 때, 그 첨가수준이 증가할수록 모두 높아졌다. 양파즙, 설탕 그리고 yeast extract를 배지성분요인으로 선택하였고, 배지 내 성분요인들의 혼합비율에 의한 효과를 평가하였다. 24 개의 실험들로 구성된 완전 요인 배치법을 이용하여 요인들의 주효과와 상호작용을 조사하였다. 균체성장과 항산화 활성에 대한 유의성 있는 긍정적 주효과는 각각 yeast extract와 양파즙에서 나타났다. 유의적인 상호작용으로는 항산화 활성에 대하여 설탕와 yeast extract 사이에서 나타났다. 최종적으로 양파 600 g/l, 설탕 15 g/l 및 효모추출물 5 g/l의 최적 배지조성을 선발하였다. 얻어진 최적배지의 효율을 분석하기 위하여 5 l 발효기를 이용하여 평가한 결과 최대 균체 성장량은 배양 12시간째에 $9.7\;{\log}_{10}$ (CFU/ml)로 나타났다. 배양 마지막인 20시간 때에는 $8.9\;{\log}_{10}$ (CFU/ml)의 균체 성장량을 보였으며 이는 예측된 $9.01\;{\log}_{10}$ (CFU/ml)과 매우 유사한 결과였다. 배양액의 항산화 활성은 모든 배양 시간대에서 약 60~65%로 유지되었으며, 이것은 예측된 값(47.1%)보다 훨씬 우수하였다.

This study was conducted to establish the optimal medium condition for the animal probiotic Lactobacillus plantarum by using onion juice. Cell yield and antioxidant activity increased in proportion to high additive levels of onion juice in medium. Onion juice, sucrose and yeast extract were selected as media ingredient factors and the effects of their mixed ratio in medium were evaluated. The full factorial design consisted of 24 experimental runs and was employed to estimate the main effects of the factors and their interactions. Significant positive effects on cell yield and antioxidant activity was shown with yeast extract and onion juice, respectively. Significant interaction was found only between sucrose and yeast extract in antioxidant activity. Finally, we selected an optimal medium that was composed of (g/l) onion juice, 600; sucrose, 15; yeast extract, 5. The efficiency of this optimum medium was estimated by using a 5 l jar fermenter. As a result, the maximum cell yield was $9.7\;{\log}_{10}$ (CFU/ml) at 12 hr. Cell yield at the end of incubation (20 hr) was $8.9\;{\log}_{10}$ (CFU/ml) and it was very similar with the predicted value, $9.0\;{\log}_{10}$ (CFU/ml). Antioxidant activity of culture was maintained at about 60~65% during all incubation time, resulting in a higher-than-predicted activity of 47.1%.

키워드

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피인용 문헌

  1. Optimization of Carbon Sources to Improve Antioxidant Activity in Solid State Fermentation of Persimmon peel Using Lactic Acid Bacteria vol.32, pp.4, 2012, https://doi.org/10.5333/KGFS.2012.32.4.361
  2. Effects of Onion Extracts on Growth Performance, Carcass Characteristics and Blood Profiles of White Mini Broilers vol.28, pp.2, 2015, https://doi.org/10.5713/ajas.14.0492