Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Induction of Physiological Sex-Reversal and Gynogenetic Diploid in Rainbow Trout, Oncorhynchus mykiss

무지개송어의 생리학적 성전환과 자성발생 2배체 유도

  • Lee, Cheul-Ho (Cold-Water Fish Research Center, National Fisheries Research & Development Institute (NFRDI)) ;
  • Kim, Dae-Jung (Inland Aquaculture Research Center, NFRDI) ;
  • Jeong, Chang-Hwa (Binex, Inc.) ;
  • Choi, Gyeong-Cheol (Chungcheongbuk-do Inland Fisheries Research Institute) ;
  • Lee, Chae-Sung (Cold-Water Fish Research Center, National Fisheries Research & Development Institute (NFRDI)) ;
  • Kim, Dong-Soo (Department of Aquaculture, Pukyong National University)
  • Received : 2010.08.06
  • Accepted : 2010.10.27
  • Published : 2010.11.30
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Abstract

This study was conducted to increase the efficiency of farming practice in rainbow trout, Oncorhynchus mykiss, by sex reversal and chromosome-set manipulation techniques. To obtain phenotypic males, hormonal sex reversal was carried out using an exogenous hormone treatment method. 5 mg of 17 alpha-methyltestosterone per kg diet was supplied for 82 days after first feeding at $10^{\circ}C$ and $13^{\circ}C$. More than 93% of the male population was produced by this method and growth of hormone-treated fish at $13^{\circ}C$ was faster than that of untreated bi-sexual groups. Induced diploid gynogenesis was carried out using artificial insemination of UV-irradiated sperm into haploid eggs. Based on the appearance of the rate of haploid syndrome and survival of embryo, a UV ray dose of at least $3,600\;erg/cm^2$ was required to inactivate rainbow trout sperm genetically. Haploid embryos were restored to diploid by blocking the extrusion of the second polar body using heat shock treatment at $28^{\circ}C$ for 20 min, 10 min post insemination. Gynogenetic diploid sex ratios were confirmed after maturation of the fish erythrocyte measurements and chromosome counts.

본 연구는 무지개송어 양식 산업의 생산성 향상을 위해 전 암컷 무지개송어 대량생산을 위한 일환으로 성호르몬에 의한 생리학적 성전환과 자성발생 2배체어를 유도하였다. 생리학적으로 성전환된 수컷을 만들기 위하여 부화 후 첫 먹이를 먹는 시기에 웅성호르몬인 17 alpha-methyltestosterone (MT) 5 mg을 사료에 흡착시켜 사육수온 $13^{\circ}C$에서 적산수온 $800^{\circ}C$까지 처리한 결과 96.7%의 수컷 유도율을 보였다. 또한 정상 수컷 정액을 이용하여 수정 10분 후 $28^{\circ}C$에서 20분간 고온 처리하여 61.7%의 자성발생 2배체가 유도되었다.

Keywords

References

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