Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Fucus evanescens Fucoidan on Expression of Matrix Metalloproteinase-1 Promoter, mRNA, Protein and Signal Pathway

Fucus evanescens fucoidan의 matrix metalloproteinase-1 promoter, mRNA, 단백질 발현과 신호전달경로에 미치는 효과

  • Ku, Mi-Jeong (Institute of Natural Products for Health Promotion and Department of Preventive Medicine, College of Medicine, Kosin University) ;
  • Jung, Ji-Won (Institute of Natural Products for Health Promotion and Department of Preventive Medicine, College of Medicine, Kosin University) ;
  • Lee, Myeong-Sook (Institute of Natural Products for Health Promotion and Department of Preventive Medicine, College of Medicine, Kosin University) ;
  • Cho, Byung-Kyu (Institute of Natural Products for Health Promotion and Department of Preventive Medicine, College of Medicine, Kosin University) ;
  • Lee, Soon-Rye (Department of Beauty Art, Institute for Continuing Education, Youngsan University) ;
  • Lee, Hye-Sook (Department of Beauty and Health Care, Daejeon University) ;
  • Vischuk, Olesya S. (Pacific Institute of Bioorganic Chemistry of Far Eastern Branch of the Russian Academy of Sciences) ;
  • Zvyagintseva, Tatyana N. (Pacific Institute of Bioorganic Chemistry of Far Eastern Branch of the Russian Academy of Sciences) ;
  • Ermakova, Svetlana P. (Pacific Institute of Bioorganic Chemistry of Far Eastern Branch of the Russian Academy of Sciences) ;
  • Lee, Yong-Hwan (Institute of Natural Products for Health Promotion and Department of Preventive Medicine, College of Medicine, Kosin University)
  • 구미정 (고신대학교 의과대학 건강증진천연물연구소 및 예방의학교실) ;
  • 정지원 (고신대학교 의과대학 건강증진천연물연구소 및 예방의학교실) ;
  • 이명숙 (고신대학교 의과대학 건강증진천연물연구소 및 예방의학교실) ;
  • 조병규 (고신대학교 의과대학 건강증진천연물연구소 및 예방의학교실) ;
  • 이순례 (영산대학교 평생교육원 미용예술학과) ;
  • 이혜숙 (대전대학교 뷰티건강관리학과) ;
  • ;
  • ;
  • ;
  • 이용환 (고신대학교 의과대학 건강증진천연물연구소 및 예방의학교실)
  • Received : 2010.09.27
  • Accepted : 2010.11.18
  • Published : 2010.11.30
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Abstract

Fucoidans are sulfated fucosylated polymers from the cell wall of brown algae. We assessed the effects of Fucus evanescens fucoidan on ultraviolet-B (UVB)-induced expression of matrix metalloproteinase-1 (MMP-1) protein, mRNA, and promoter, and the phosphorylation of mitogen-activated protein kinases in vitro using an immortalized human keratinocyte cell line. Pretreatment with 10 and $100\;{\mu}g/ml$ fucoidan significantly inhibited UVB-induced MMP-1 protein, mRNA and promoter activity, compared to UVB irradiation alone. Extracellular signal regulated kinase activation was markedly inhibited by treatment with fucoidan, though c-JUN N-terminal kinase activity and p38 activation were only marginally affected by fucoidan. F. evanescens fucoidan may be a potential therapeutic agent for the prevention and treatment of skin photoaging.

Fucoidan은 갈조류의 세포벽에 존재하는 황산화 다당류이다. 본 연구에서는 자외선 B를 인체각질형성세포에 조사하여 matrix metalloproteinase-1 (MMP-1)을 발현 시킨 후 Fucus evanescens fucoidan이 MMP-1 promoter, mRNA, 단백 발현과 mitogen-activated protein kinases (MAPKs)의 인산화에 미치는 영향을 확인하고자 하였다. 자외선 B에 의해 생성된 MMP-1의 promoter activity와 mRNA, 단백 발현은 fucoidan $10\;{\mu}g/ml$와 $100\;{\mu}g/ml$를 투여하였을 때 fucoidan을 투여하지 않고 자외선만 조사한 군에 비하여 유의하게 억제되었다. 그리고 F. evanescens fucoidan은 extracellular signal regulated kinase (ERK)의 활성은 현저히 억제시켰으나 c-JUN N-terminal kinase (JNK)와 p38의 활성에 미치는 영향은 약하였다. 따라서 이 연구결과들은 F. evanescens fucoidan이 피부 광노화의 예방과 치료에 도움이 될 가능성을 확인할 수 있었다.

Keywords

References

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