생명과학회지 (Journal of Life Science)

  • 제20권11호
  • /
  • Pages.1603-1610
  • /
  • 2010
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

Fucus evanescens fucoidan의 matrix metalloproteinase-1 promoter, mRNA, 단백질 발현과 신호전달경로에 미치는 효과

Effect of Fucus evanescens Fucoidan on Expression of Matrix Metalloproteinase-1 Promoter, mRNA, Protein and Signal Pathway

  • 구미정 (고신대학교 의과대학 건강증진천연물연구소 및 예방의학교실) ;
  • 정지원 (고신대학교 의과대학 건강증진천연물연구소 및 예방의학교실) ;
  • 이명숙 (고신대학교 의과대학 건강증진천연물연구소 및 예방의학교실) ;
  • 조병규 (고신대학교 의과대학 건강증진천연물연구소 및 예방의학교실) ;
  • 이순례 (영산대학교 평생교육원 미용예술학과) ;
  • 이혜숙 (대전대학교 뷰티건강관리학과) ;
  • ;
  • ;
  • ;
  • 이용환 (고신대학교 의과대학 건강증진천연물연구소 및 예방의학교실)
  • Ku, Mi-Jeong (Institute of Natural Products for Health Promotion and Department of Preventive Medicine, College of Medicine, Kosin University) ;
  • Jung, Ji-Won (Institute of Natural Products for Health Promotion and Department of Preventive Medicine, College of Medicine, Kosin University) ;
  • Lee, Myeong-Sook (Institute of Natural Products for Health Promotion and Department of Preventive Medicine, College of Medicine, Kosin University) ;
  • Cho, Byung-Kyu (Institute of Natural Products for Health Promotion and Department of Preventive Medicine, College of Medicine, Kosin University) ;
  • Lee, Soon-Rye (Department of Beauty Art, Institute for Continuing Education, Youngsan University) ;
  • Lee, Hye-Sook (Department of Beauty and Health Care, Daejeon University) ;
  • Vischuk, Olesya S. (Pacific Institute of Bioorganic Chemistry of Far Eastern Branch of the Russian Academy of Sciences) ;
  • Zvyagintseva, Tatyana N. (Pacific Institute of Bioorganic Chemistry of Far Eastern Branch of the Russian Academy of Sciences) ;
  • Ermakova, Svetlana P. (Pacific Institute of Bioorganic Chemistry of Far Eastern Branch of the Russian Academy of Sciences) ;
  • Lee, Yong-Hwan (Institute of Natural Products for Health Promotion and Department of Preventive Medicine, College of Medicine, Kosin University)
  • 투고 : 2010.09.27
  • 심사 : 2010.11.18
  • 발행 : 2010.11.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Fucoidan은 갈조류의 세포벽에 존재하는 황산화 다당류이다. 본 연구에서는 자외선 B를 인체각질형성세포에 조사하여 matrix metalloproteinase-1 (MMP-1)을 발현 시킨 후 Fucus evanescens fucoidan이 MMP-1 promoter, mRNA, 단백 발현과 mitogen-activated protein kinases (MAPKs)의 인산화에 미치는 영향을 확인하고자 하였다. 자외선 B에 의해 생성된 MMP-1의 promoter activity와 mRNA, 단백 발현은 fucoidan $10\;{\mu}g/ml$와 $100\;{\mu}g/ml$를 투여하였을 때 fucoidan을 투여하지 않고 자외선만 조사한 군에 비하여 유의하게 억제되었다. 그리고 F. evanescens fucoidan은 extracellular signal regulated kinase (ERK)의 활성은 현저히 억제시켰으나 c-JUN N-terminal kinase (JNK)와 p38의 활성에 미치는 영향은 약하였다. 따라서 이 연구결과들은 F. evanescens fucoidan이 피부 광노화의 예방과 치료에 도움이 될 가능성을 확인할 수 있었다.

Fucoidans are sulfated fucosylated polymers from the cell wall of brown algae. We assessed the effects of Fucus evanescens fucoidan on ultraviolet-B (UVB)-induced expression of matrix metalloproteinase-1 (MMP-1) protein, mRNA, and promoter, and the phosphorylation of mitogen-activated protein kinases in vitro using an immortalized human keratinocyte cell line. Pretreatment with 10 and $100\;{\mu}g/ml$ fucoidan significantly inhibited UVB-induced MMP-1 protein, mRNA and promoter activity, compared to UVB irradiation alone. Extracellular signal regulated kinase activation was markedly inhibited by treatment with fucoidan, though c-JUN N-terminal kinase activity and p38 activation were only marginally affected by fucoidan. F. evanescens fucoidan may be a potential therapeutic agent for the prevention and treatment of skin photoaging.

키워드

참고문헌

  1. Alekseyenko, T. V., S. Y. Zhanayeva, A. A. Venediktova, T. N. Zvyagintseva, T. A. Kuznetsova, N. N. Besednova, and T. A. Korolenko. 2007. Antitumor and antimetastatic activity of fucoidan, a sulfated polysaccharide isolated from the Okhotsk Sea Fucus evanescens brown alga. Bull. Exp. Biol. Med. 143, 730-732. https://doi.org/10.1007/s10517-007-0226-4
  2. Anastyuk, S. D., N. M. Shevchenko, E. L. Nazarenko, P. S. Dmitrenok, and T. N. Zvyagintseva. 2009. Structural analysis of a fucoidan from the brown alga Fucus evanescens by MALDI-TOF and tandem ESI mass spectrometry. Carbohydr. Res. 344, 779-787. https://doi.org/10.1016/j.carres.2009.01.023
  3. Benbow, U. and C. E. Brinckerhoff. 1997. The AP-1 site and MMP gene regulation: what is all the fuss about? Matrix Biol. 15, 519-526. https://doi.org/10.1016/S0945-053X(97)90026-3
  4. Bilan, M. I., A. A. Grachev, N. E. Ustuzhanina, A. S. Shashkov, N. E. Nifantiev, and A. I. Usov. 2002. Structure of a fucoidan from the brown seaweed Fucus evanescens C. Ag. Carbohydr. Res. 337, 719-730. https://doi.org/10.1016/S0008-6215(02)00053-8
  5. Boukamp, P., R. T. Dzarlieva-Petrusevska, D. Breitkreuz, J. Hornung, A. Markham, and N. E. Fuseing. 1988. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J. Biol. Chem. 106, 761-771.
  6. Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
  7. Chung, J. H., J. Y. Seo, M. K. Lee, H. C. Eun, J. H. Lee, S. Kang, G. J. Fisher, and J. J. Voorhees. 2002. Ultraviolet modulation of human macrophage metalloelastase in human skin in vivo. J. Invest. Dermatol. 119, 507-512. https://doi.org/10.1046/j.1523-1747.2002.01844.x
  8. Cumashi, A., N. A. Ushakova, M. E. Preobrazhenskaya, A. D'Incecco, A. Piccoli, L. Totani, N. Tinari, G. E. Morozevich, A. E. Berman, M. I. Bilan, A. I. Usov, N. E. Ustyuzhanina, A. A. Grachev, C. J. Sanderson, M. Kelly, G. A. Rabinovich, S. Iacobelli, and N. E. Nifantiev. 2007. A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds. Glycobiology 17, 541-552.
  9. Dodgson, K. S. and R. G. Price. 1962. A note on the determination of the ester sulfate content of sulfated polysaccharides. Biochem. J. 84, 106-110.
  10. DuBois, M., K. Gilles, J. Hamilton, P. Roberts, and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350-356. https://doi.org/10.1021/ac60111a017
  11. Fisher, G. J., S. C. Datta, H. S. Talwar, Z. Q. Wang, J. Varani, S. Kang, and J. J. Voorhees. 1996. Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature 379, 335-339. https://doi.org/10.1038/379335a0
  12. Fisher, G. J., Z. Q. Wang, S. C. Datta, J. Varani, S. Kang, and J. J. Voorhees. 1997. Pathophysiology of premature skin aging induced by ultraviolet light. N. Engl. J. Med. 337, 1419-1428. https://doi.org/10.1056/NEJM199711133372003
  13. Fisher, G. J., H. S. Talwar, J. Lin, P. Lin, F. McPhillips, Z. Wang, X. Li, Y. Wan, S. Kang, and J. J. Voorhees. 1998. Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occurs subsequent to activation of mitogen-activated protein kinase pathways in human skin in vivo. J. Clin. Invest. 101, 1432-1440. https://doi.org/10.1172/JCI2153
  14. Ho, J. N., Y. H. Lee, Y. D. Lee, W. J. Jun, H. K. Kim, B. S. Hong, D. H. Shin, and H. Y. Cho. 2005. Inhibitory effect of Aucubin isolated from Eucommia ulmoides against UVB-induced matrix metalloproteinase-1 production in human skin fibroblasts. Biosci. Biotechnol. Biochem. 69, 2227-2231. https://doi.org/10.1271/bbb.69.2227
  15. Jenkins, G. 2002. Molecular mechanisms of skin ageing. Mech. Ageing Dev. 123, 801-810. https://doi.org/10.1016/S0047-6374(01)00425-0
  16. Joe, M. J., S. U. Kim, H. Y. Choi, W. S. Shin, G. M. Park, D. W. Kang, and Y. K. Kim. 2006. The inhibitory effects of eckol and dieckol from Ecklonia stolonifera on the expression of matrix metalloproteinase-1 in human dermal fibroblasts. Biol. Pharm. Bull. 29, 1735-1739. https://doi.org/10.1248/bpb.29.1735
  17. Kim, H. H., C. M. Shin, C. H. Park, K. H. Kim, K. H. Cho, H. C. Eun, and J. H. Chung. 2005. Eicosapentaenoic acid inhibits UV-induced MMP-1 expression in human dermal fibroblasts. J. Lipid Res. 46, 1712-1720. https://doi.org/10.1194/jlr.M500105-JLR200
  18. Kusaykin, M. I., A. O. Chizhov, A. A. Grachev, S. A. Alekseeva, I. Y. Bakunina, O. I. Nedashkovskaya, V. V. Sova, and T. N. Zvyagintseva. 2006. A comparative study of specificity of fucoidanases from marine microorganisms and invertebrates. J. Appl. Phycol. 18, 369-373. https://doi.org/10.1007/s10811-006-9042-x
  19. Kuznetsova, T. A., N. N. Besednova, A. N. Mamaev, A. P. Momot, N. M. Shevchenko, and T. N. Zvyagintseva. 2003. Anticoagulant activity of fucoidan from brown algae Fucus evanescens of the Okhotsk Sea. Bull. Exp. Biol. Med. 136, 471-473. https://doi.org/10.1023/B:BEBM.0000017096.72246.1f
  20. Kylin, H. 1913. Zur Biochemie der Meeresalgen. Z. Physiol. Chemie. 83, 171-197. https://doi.org/10.1515/bchm2.1913.83.3.171
  21. Lapikova, E. S., N. N. Drozd, A. S. Tolstenkov, V. A. Makarov, T. N. Zvyagintseva, N. M. Shevchenko, I. U. Bakunina, N. N. Besednova, and T. A. Kuznetsova. 2008. Inhibition of thrombin and factor Xa by Fucus evanescens fucoidan and its modified analogs. Bull. Exp. Biol. Med. 146, 328-333. https://doi.org/10.1007/s10517-008-0267-3
  22. Makarenkova, I. D., G. G. Kompanets, N. N. Besednova, R. A. Slonova, T. N. Zviagintseva, and N. M. Shevchenko. 2008. Inhibiting effects of fucoidans on Hantaan virus adsorption on a model of peritoneal macrophages in vitro. Vopr. Virusol. 53, 12-15. (Russian).
  23. Li, B., F. Lu, X. Wei, and R. Zhao. 2008. Fucoidan: structure and bioactivity. Molecules 13, 1671-1695. https://doi.org/10.3390/molecules13081671
  24. Lim, H. and H. P. Kim. 2007. Inhibition of mammalian collagenase, matrix metalloproteinase-1, by naturally-occurring flavonoids. Planta Med. 73, 1267-1274. https://doi.org/10.1055/s-2007-990220
  25. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
  26. McLellan, D. S. and K. M. Jurd. 1992. Anticoagulants from marine algae. Blood Coagul. Fibrinolysis 3, 69-77. https://doi.org/10.1097/00001721-199202000-00011
  27. Moon, H. I., M. R. Kim, E. R. Woo, and J. H. Chung. 2005. Triterpenoid from Styrax japonica SIEB. et ZUCC, and its effects on the expression of matrix metalloproteinases-1 and type 1 procollagen caused by ultraviolet irradiated cultured primary human skin fibroblasts. Biol. Pharm. Bull. 28, 2003-2006. https://doi.org/10.1248/bpb.28.2003
  28. Moon, H. J., S. R. Lee, S. N. Shim, S. H. Jeong, V. A. Stonik, V. A. Rasskazov, T. Zvyagintseva, and Y. H. Lee. 2008. Fucoidan inhibits UVB-induced MMP-1 expression in human skin fibroblasts. Biol. Pharm. Bull. 31, 284-289. https://doi.org/10.1248/bpb.31.284
  29. Murphy, K. A., C. M. Villano, R. Dorn, and L. A. White. 2004. Interaction between the aryl hydrocarbon receptor and retinoic acid pathways increases matrix metalloproteinase-1 expression in keratinocytes. J. Biol. Chem. 279, 25284-25293. https://doi.org/10.1074/jbc.M402168200
  30. Quan, T., T. He, S. Kang, J. J. Voorhees, and G. J. Fisher. 2004. Solar ultraviolet irradiation reduces collagen in photoaged human skin by blocking transforming growth factor-beta type II receptor/Smad signaling. Am. J. Pathol. 165, 741-751. https://doi.org/10.1016/S0002-9440(10)63337-8
  31. Senni, K., F. Gueniche, A. Foucault-Bertaud, S. Igondjo-Tchen, F. Fioretti, S. Colliec-Jouault, P. Durand, J. Guezennec, G. Godeau, and D. Letourneur. 2006. Fucoidan a sulfated polysaccharide from brown algae is a potent modulator of connective tissue proteolysis. Arch. Biochem. Biophys. 445, 56-64. https://doi.org/10.1016/j.abb.2005.11.001
  32. Sun, Y., Y. Sun, L. Wenger, J. L. Rutter, C. E. Brinckerhoff, and H. S. Cheung. 1999. p53 down-regulates human matrix metalloproteinase-1 (Collagenase-1) gene expression. J. Biol. Chem. 274, 11535-11540. https://doi.org/10.1074/jbc.274.17.11535
  33. Zvyagintseva, T. N., N. M. Shevchenko, E. L. Nazarenko, V. I. Gorbach, A. M. Urvantseva, M. I. Kiseleva, and V. V. Isakov. 2005. Water-soluble polysaccharides of some brown algae of the Russian Far-East. Structure and biological action of low-molecular mass polyuronans. J. Exp. Mar. Biol Ecol. 320, 123-131. https://doi.org/10.1016/j.jembe.2004.12.027

피인용 문헌

  1. Fucoidans from Brown Alga Fucus evanescens: Structure and Biological Activity vol.3, 2016, https://doi.org/10.3389/fmars.2016.00129
  2. A mechanism of low molecular weight fucoidans degraded by enzymatic and acidic hydrolysis for the prevention of UVB damage vol.29, pp.1, 2017, https://doi.org/10.1007/s10811-016-0929-x
  3. In vitro anticancer activity of the laminarans from Far Eastern brown seaweeds and their sulfated derivatives vol.29, pp.1, 2017, https://doi.org/10.1007/s10811-016-0915-3
  4. Phycochemical Constituents and Biological Activities of Fucus spp. vol.16, pp.8, 2018, https://doi.org/10.3390/md16080249