Mycobiology

The Korean Society of Mycology (한국균학회)
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Development of Detection Methods for Cellulolytic Activity of Auricularia auriculajudae

  • Jo, Woo-Sik (Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration) ;
  • Bae, Soon-Hwa (Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration) ;
  • Choi, Seung-Yong (Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration) ;
  • Park, So-Deuk (Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration) ;
  • Yoo, Young-Bok (Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horicultural and Herbal Science, RDA) ;
  • Park, Seung-Chun (College of Veterinary Medicine, Kyeongbuk National University)
  • Published : 2010.03.31
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Abstract

To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from $15\sim35^{\circ}C$. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at $25^{\circ}C$ for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.

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References

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