Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant and Anti-diabetes Activities of Methanolic Extract and Fractions of Astragalus membranaceus Roots

  • Park, Jae-Hyo (Department of Neurosurgery, Kangwon National University Hospital, School of Medicine, Kangwon National University) ;
  • Yin, Yu (College of Biomedical Science, Kangwon National University) ;
  • Wang, Myeong-Hyeon (College of Biomedical Science, Kangwon National University)
  • Published : 2010.03.31
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Abstract

The potential biological activities of methanol extract and 5 fractions (hexane, $CH_2Cl_2$, EtOAc, BuOH and water) from roots of Astragalus membranaceus were examined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, hydroxyl radical (${\cdot}OH$) scavenging activity, reducing power assays, lipid peroxidation inhibitory activity, $\alpha$-amylase and $\alpha$-glucosidase inhibition assays. The EtOAc fraction showed high DPPH free radical scavenging activity ($EC_{50}=170.34\;{\mu}g/mL$), hydroxyl radical scavenging activity ($EC_{50}=32.14\;{\mu}g/mL$), lipid peroxidation inhibitory activity ($EC_{50}=52.46\;{\mu}g/mL$) and a concentration dependence, with OD value ranging from 0.234 to 0.345 (0.1 to 0.5 mg/mL), for reducing power. The EtOAc fraction has the highest total phenolic content ($142.13\;Gal\;{\mu}g/mg$) and the $CH_2Cl_2$ fraction has the highest flavonoid content ($71.63\;Que\;{\mu}g/mg$). Meanwhile, hexane and EtOAc showed certain $\alpha$-amylase and $\alpha$-glucosidase inhibition activities. These results suggest that the methanol extract and fractions from Astragalus membranaceus root have significant antioxidant and anti-diabetes activities, which could be used as a potential source of pharmaceutical materials.

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References

  1. Liu JK, Head E, Gharib AM, Yuan WJ, Ingersell RT, Hagen TM, Cotman CW, Ames BN. 2002. Memory loss in old rats is associated with brain mitochondrial decay and RNA/DNA oxidation: partial reversal by feeding acetyl-L-carnitine and /or R-$\alpha$-lipoic acid. Proc Natl Acad Sci USA 99: 2356-7185. https://doi.org/10.1073/pnas.261709299
  2. Ahmad P, Sarwat M, Sharma S. 2008. Reactive oxygen species, antioxidants and signaling in plants. J Plant Biol 51: 167-173. https://doi.org/10.1007/BF03030694
  3. Wood KC, Hsu LL, Gladwin MT. 2008. Sickle cell disease vasculopathy: a state of nitric oxide resistance. Free Radic Biol Med 44: 1506-1528. https://doi.org/10.1016/j.freeradbiomed.2008.01.008
  4. Holman RR, Cull CA, Turner RC. 1999. A randomized double-blind trial of acarbose in type 2 diabetes shows improved glycemic control over 3 years (U.K. Prospective Diabetes Study 44). Diabetes Care 22: 960-964. https://doi.org/10.2337/diacare.22.6.960
  5. Ells LJ, Seal CJ, Kettliz B, Bal W, Mathers C. 2005. Postprandial glycaemic, lipaemic and haemostatic responses to ingestion of rapidly and slowly digested starches in healthy young women. Br J Nutr 94: 948-955. https://doi.org/10.1079/BJN20051554
  6. Shi JF, Zhu HW, Zhang C, Bian F, Shan JP, Lu WL. 2002. Therapeutic effect of Astragalus on patients with chronic glomerulonephritis. Acta Univ Med Sec Shanghai 22: 245-248.
  7. Wang YP, Li XY, Song CQ, Hu ZB. 2002. Effect of astragaloside IV on T, B lymphocyte proliferation and peritoneal macrophage function in mice. Acta Pharmacol Sin 23: 263-266.
  8. Zhao YQ, Li GQ, Guo CX, Lian X. 2000. Evaluation the effect of TNF-alpha, RBC immunologic function and improvement renal function by Astragalus root in patients with chronic renal failure. J Mudanjiang Med Coll 21: 5-6.
  9. Lui S, Luo X, Li D, Zhang J, Qui D, Lui W, She L, Yang Z. 2006. Tumor inhibition and improve immunity in mice treated with flavone from Cirsium japonicum DC. Int Immunopharmacol 6: 1387-1393. https://doi.org/10.1016/j.intimp.2006.02.002
  10. Lin JY, Tang CY. 2007. Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chem 101: 140-147. https://doi.org/10.1016/j.foodchem.2006.01.014
  11. Yoshida T, Mori K, Hatano T, Uehara I, Komagoe K, Fujita Y, Okuda T. 1989. Study on inhibition mechanism of antioxidation by tannins and flavonoids. V. Radical scavenging effects of tannins and related polyphenols on 1,1-diphenyl-2-picrylhydrazyl radical. Chem Pharm Bull 37: 1919-1921. https://doi.org/10.1248/cpb.37.1919
  12. Yin Y, Heo SI, Roh KS, Wang MH. 2009. Biological activities of fractions from methanolic extract of Picrasma quassioides. J Plant Biol 52: 325-331. https://doi.org/10.1007/s12374-009-9042-x
  13. Maeda K, Kakabayashi S, Matsubara H. 1985. Complete amino acid sequence of an α-amylase inhibitor in wheat kernel (0.19-inhibitor). Biochim Biophys Acta 828: 213-221. https://doi.org/10.1016/0167-4838(85)90299-7
  14. Kim YM, Jeong YK. 2005. Inhibitory effect of pine extract on α-glucosidase activity and postprandial hyperglycemia. Nutrition 21: 756-761. https://doi.org/10.1016/j.nut.2004.10.014
  15. Ding T, Rahman SME, Purev U, Oh DH. 2010. Modelling of Escherichia coli O157:H7 growth at various storage temperatures on beef treated with electrolyzed oxidizing water. J Food Process Eng 97: 497-503. https://doi.org/10.1016/j.jfoodeng.2009.11.007
  16. Zhao MM, Yang B, Wang JS, Li BZ, Jiang YM. 2006. Identification of the major flavonoids from pericarp tissues of lychee fruit in relation to their antioxidant activities. Food Chem 98: 539-544. https://doi.org/10.1016/j.foodchem.2005.06.028
  17. Siriwardhana SSKW, Shahidi F. 2002. Antiradical activity of extracts of almond and its by-products. JAOSC 79: 903-908. https://doi.org/10.1007/s11746-002-0577-4
  18. Amin A, Yazdanparast R. 2007. Antioxidant and free radical scavenging potential of Achillea santolina extracts. Food Chem 104: 21-29. https://doi.org/10.1016/j.foodchem.2006.10.066
  19. Prasad NK, Divakar S, Shivamurthy GR, Aradhya SM. 2005. Isolation of a free radical scavenging antioxidant from water spinach (Ipomoea aquatica Forsk). J Sci Food Agric 85: 1461-1468. https://doi.org/10.1002/jsfa.2125
  20. Duh PD. 1998. Antioxidant activity of burdock (Arctium lappa Linne): its scavenging effect on free radical and active oxygen. JAOCS 75: 455-461. https://doi.org/10.1007/s11746-998-0248-8
  21. Gordon MH. 1990. The mechanism of antioxidant action in vitro. In Food Antioxidants. Hudson BJF, ed. Elsevier Applied Science, London, UK. p 1-18.
  22. Puls W, Keup U, Krause HP, Thomas G, Hoffmeister F. 1977. Glucosidase inhibition. A new approach to the treatment of diabetes, obesity, and hyperlipoproteinaemia. Naturwissenschaften 64: 536-537. https://doi.org/10.1007/BF00483562

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