Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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7, 8, 4'-Trihydroxyisoflavone from Fermented Soybean Food and Its Biological Activity

전통 콩발효 식품 유래 7, 8, 4'-Trihydroxyisoflavone의 피부 생리활성 연구

  • 박준성 ((주)아모레퍼시픽 기술연구원 피부과학연구소) ;
  • 김동현 ((주)아모레퍼시픽 기술연구원 피부과학연구소) ;
  • 심진섭 ((주)아모레퍼시픽 기술연구원 피부과학연구소) ;
  • 김지성 ((주)아모레퍼시픽 기술연구원 피부과학연구소) ;
  • 최권영 (서울대학교 화학생물공학부) ;
  • 김병기 (서울대학교 화학생물공학부) ;
  • 김덕희 ((주)아모레퍼시픽 기술연구원 피부과학연구소) ;
  • 김한곤 ((주)아모레퍼시픽 기술연구원 피부과학연구소)
  • Received : 2010.09.10
  • Accepted : 2010.09.25
  • Published : 2010.09.30
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Abstract

A rare isoflavone, 7, 8, 4'-trihydroxyisoflavone, was isolated from a 10-year-old fermented soybean food. 7, 8, 4'-trihydroxyisoflavone was isolated for the first time from a Korean fermented soybean food. Evaluation tests of biological activity showed significantly inhibition activity for free radical scavenging on both non-enzymatic (DPPH system) and enzymatic method (xanthine oxidase system). DPPH radical scavenging effect of 7, 8, 4'-trihydroxyisoflavone was similar with vitamin C in a dose-dependent manner. In xanthine oxidase (XO) system 7, 8, 4'-trihydroxyisoflavone showed superoxide radical inhibition activity of 50 % at a concentration of $6.6{\pm}0.4\;{\mu}M$. Also, the compound significantly suppressed cellular MMP-1 formation. These results suggest that 7, 8, 4'-trihydroxyisoflavone could be developed as a potential preventive or therapeutic agent against skin aging.

10년 이상 자연 발효된 콩 발효 식품에서 희귀 이소플라본 성분인 7, 8, 4'-trihydroxyisoflavone을 분리하였고, 분리된 성분의 항산화 효과를 비효소적인 방법으로는 DPPH 법을, 효소적인 방법으로는 xanthine oxidase법을 이용하여 확인하였다. 그 결과 7, 8, 4'-trihydroxyisoflavone는 농도 의존적으로 비타민 C와 유사한 DPPH 라디칼 소거 효능을 가지고 있음을 보여주었고 $6.6{\pm}0.4\;{\mu}M$ 농도에서 xanthine oxidase에서 생성된 superoxide 라디칼을 50 % 수준까지 낮춤을 보여주었다. 또한, 이 희귀 이소플라본 성분은 UVB로 유도된 MMP-1의 생성을 초기 수준으로 억제하는 것으로 나타나 피부 노화 억제에 유용한 성분임을 확인할 수 있었다.

Keywords

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