Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Proteomic Analysis of Differentially Expressed Plasma Proteins during Early Pregnancy in Hanwoo

한우에서 임신 초기 발현 차이 혈장 단백질의 단백질체학적 분석

  • 김평희 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 권대진 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 오건봉 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 이휘철 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 양병철 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 임기순 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 민관식 (한경대학교 생물환경정보통신전문대학원) ;
  • 윤종택 (한경대학교 생물환경정보통신전문대학원) ;
  • 진동일 (충남대학교 축산학과) ;
  • 박수봉 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 황성수 (농촌진흥청 국립축산과학원 동물바이오공학과)
  • Received : 2010.09.10
  • Accepted : 2010.09.17
  • Published : 2010.09.30
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Abstract

This study was performed to comprehend the plasma proteins expressed specifically during early pregnancy in pregnant or non-pregnant Hanwoo using proteomic analysis technique. Plasma samples (0, 2, 3, 4, 7, and 11 weeks after AI) were obtained from pregnant (P, n=3) or non-pregnant (NP, n=4) Hanwoo, respectively. To evaluate proteins differentially expressed, 2-dimensional electrophoresis (2DE) was conducted. Normalized protein spots were selected for the significant expression variation deviated over two fold in its expression level between two groups; Molecular functions of the proteins were DNA binding, protein binding, hemoglobin binding, ferrochelatase and transporter activity and arylestera, respectively. According to western blotting, haptoglobin was specifically expressed only in NP group during early pregnancy; however, paraoxonase 1 was highly expressed in pregnant group. Based on these results, pregnancy was maintained successfully by the activation of specific plasma proteins associated with immune system and antioxidant regulation during early pregnancy in Hanwoo.

Keywords

References

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