대한의생명과학회지 (Biomedical Science Letters)

  • 제16권3호
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  • Pages.187-192
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  • 2010
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
권호 표지

Molecular Identification Patterns of Clinical Isolates from Korean Patients Infected with Dermatopbytes

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  • 김영권 (건양대학교 의과학대학 임상병리학과)
  • Kim, Sang-Ha (Department of Public Health and Welfare, Graduate School of Konyang University) ;
  • Cho, Seong-Wan (Department of Pharmaceutical Engineering, Konyang University) ;
  • Hwang, Seock-Yeon (Department of Biomedical Laboratory Science, Daejeon University) ;
  • Jeon, Soo-Young (Department of Dermatology, Konyang University College of Medicine) ;
  • Kim, Young-Kwon (Department of Biomedical Laboratory Science, Konyang University College of Biomedical Science)
  • 투고 : 2009.11.16
  • 심사 : 2010.09.27
  • 발행 : 2010.09.30
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초록

The aim of this study was to compare the results of morphological phenotypic examination and molecular biological method, randomly amplified polymorphic DNA (RAPD) using clinical isolates from Korean patients infected with dermatophytes. A total of 44 clinical isolates have been collected by Korean Collection Medical Fungi (KCMF) and those were subjected for the RAPD analysis. The results showed that the fungal strains that have RG1 band patterns were identified as Trichophyton rubrum by the neighbour-joining analysis. Additionally, other fungal strains that have RG3 band patterns were identified as Epidermophyton floccosum and RG5 band patterns were identified as Micorsporum gypseum. Even though molecular biological method such as RAPD are not necessary, it might be useful when some strains have similar morphological characteristics or when same species has phenotypic variations. In this study, therefore, we targeted internal transcribed spacer (ITS) regions of fungal rDNA, performed RAPD and then compared with morphological phenotypic examination.

키워드

참고문헌

  1. Aly R, Hay RJ, Del Palacio A, Galimberti R. Epidemiology of tinea capitis. Med Mycol. 2000. 38: 183-188. https://doi.org/10.1080/mmy.38.s1.183.188
  2. Aman S, Haroon TS, Hussain I, Bokhari MA, Khurshid K. Tinea unguium in Lahore, Pakistan. Med Mycol. 2001. 39: 177-180. https://doi.org/10.1080/mmy.39.2.177.180
  3. Arenas R. Dominguez-Cherit J, Fernandez LM. Open randomized comparison of itraconazole versus terbinafine in onychomycosis. Int J Dermatol. 1995. 34: 138-143. https://doi.org/10.1111/j.1365-4362.1995.tb03600.x
  4. Clinical and Laboratory Standard Institute. Interpretative criteria for identification of bacteria and fungi by DNA target sequencing; approved guideline. MM18-A pl-71, Pennsylvania Clinical and Laboratory Standard Institute, 2008.
  5. Elewski BE. Tinea capitis: a current perspective. J Am Acad Dermatol. 2000. 42: 1-20; quiz 21-24. https://doi.org/10.1016/S0190-9622(00)90001-X
  6. Evans EGV. Causative pathogens in onychomycosis and the possibility of treatment resistance: A review. J Amer Acad Dermatol. 1998. 38: S32-S36. https://doi.org/10.1016/S0190-9622(98)70481-5
  7. Hamelin RC, Ouellette GB, Bernier L. Identification of Bremmeniella abietina races with random amplified polymorphic DNA markers. Appl Environ Microbiol. 1993. 59: 1752-1755.
  8. Kimura M. A simple method for estimation evolutionary rate of base substiturions through comparative studies of nucleotide sequences. J Mol Evol. 1980. 16: 111-120. https://doi.org/10.1007/BF01731581
  9. Lui D, Coloe S, Baird PR Use of arbitrarily primed polymerase chain reaction to differentiate Trichophyton mentagrophytes. FEMS Microbiol Lett. 1996. 136: 147-150. https://doi.org/10.1111/j.1574-6968.1996.tb08040.x
  10. Mazurek GH, Reddy V, Narston BJ, Haas WH, Crawford JT. DNA fingerprinting by infrequentrestirction site amplification. J Dlin Microbiol. 1996. 34: 2386-2390.
  11. Mitchell TG, Sandin RL, Bowman BH. Molecular mycology: DNA probes and application of PCR technology. J Med Vet Mycol. 1999. 4: 32 (suppl. 1): 351-366.
  12. Nweze EI. Etiology of dermatophytoses amongst children in northeastern Nigeria. Med Mycol. 2001. 39: 181-184. https://doi.org/10.1080/mmy.39.2.181.184
  13. Roldan YB, Mata-Essayag S, Hartung C. Erysipelas and tinea pedis. Mycoses. 2000. 43: 181-183. https://doi.org/10.1046/j.1439-0507.2000.00553.x
  14. Rubio-Calvo C, Gil-Tomas J, Rezusta-Lopez A, Benito-Ruesca R. The aetiological agents of tinea capitis in Zaragoza (Spain). Mycoses. 2001. 44: 55-58. https://doi.org/10.1046/j.1439-0507.2001.00617.x
  15. Sabota J, Brodell R. Rutecki GW, Hoppes WL. Severe tinea barbae due to Trichophyton verrucosum infection in daity farmers. Clin Infect Dis. 1996. 23: 1308-1310. https://doi.org/10.1093/clinids/23.6.1308
  16. Thompson JD, Gibsion TJ, Plewniak F, Jeanmougin F, Higgins D, C. The Clustal X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997. 24: 4876-4882.
  17. Welsh J, NcClelland M. Fingerprinting genomes using PCR with arbitraly. Nucleic Acids Res. 1990. 18: 7213-7218. https://doi.org/10.1093/nar/18.24.7213
  18. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. pp. 315-322 In: PCR Protocols: A Guide to Methods and Applications, eds. Innis, M. A, D. H. Gelfand, J. J. Sninsky, and T. J. White. Academic Press, Inc., New York, 1990.
  19. Williams JGK, Kubelik AR. Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisrns amplified by arbritaty primers are useful as genetic markers. Nucleic Acids Res. 1990. 18: 6531-6535. https://doi.org/10.1093/nar/18.22.6531