Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Effect of Culture Condition on Hanwoo Embryonic Developments and Their Sunrival after Vitrification

배양 조건에 따른 한우 수정란의 발달과 초자화 동결 후 수정란의 생존성 비교

  • Cho, Sang-Rae (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Choi, Sun-Ho (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Choe, Chang-Yong (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Son, Jun-Kyu (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Lee, Poong-Yeon (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Ko, Yeoung-Kyu (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Hyun-Jong (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Yeon, Sung-Heum (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Son, Dong-Soo (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
  • 조상래 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 최선호 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 최창용 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 손준규 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 이풍연 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 고응규 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김현종 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 연성흠 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 손동수 (농촌진흥청 국립축산과학원 가축유전자원시험장)
  • Received : 2010.08.19
  • Accepted : 2010.08.27
  • Published : 2010.09.30
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Abstract

We investigated the cleavage rate and blastocyst yield for each culture condition to enhance tolerance of cryo-preservation of bovine IVF embryo with relatively lower cryo-tolerance compared to in vivo embryo. The cleavage rate and blastocysts yield for CR1aa, IVMD, IVD, CR1aa+10% FBS were 73.2, 69.3, 72.8, 68.5% and 44.1, 30.8, 33.3, 48.0%, respectively. The values did not differ among each treatments without serum. For embryo vitrification, In vivo and In vitro blastocysts were exposed to VS1(10% glycerin, 0.1 M glucose, 0.1 M sucrose, PEG 1%) for 5 min, and VS2 (10% glycerin, 10% EG, 0.2 M glucose, 0.2 M sucrose, PEG 2%) for 5 min and then VS3 (10% glycerin, 30% EG, 0.3 M glucose, 0.3 M sucrose, PEG 3%) for 1 min. The exposed embryos were then loaded into the 0.25 ml plastic straws and then plunged into liquid nitrogen. The straws were held for period of 1 to 2 weeks before thawing. In embryo viability, no differences in blastocyst re-expansion rates were found between in vivo and in vitro embryos. whereas expansion-BL rates was significantly higher for in vivo-derived embryos (72.7%) when compared to in vitro-derived embryos (51.4%), respectively (P<0.05). In conclusion, our results indicate that combined use of CRIaa culture medium with vitrification might enhance tolerance of cryopreservation for bovine IVF embryo production.

Keywords

References

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