치위생과학회지 (Journal of dental hygiene science)

  • 제10권3호
  • /
  • Pages.161-165
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  • 2010
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  • 1598-4478(pISSN)
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  • 2233-7679(eISSN)
한국치위생과학회 (The Korean Society of Dental Hygiene Science)
권호 표지

(-)-Epigalocatechin의 Fusobacterium nucleatum, Prevotella intermedia 및 Porphyromonas gingivalis에 대한 항균 효과

Antimicrobial effect of (-)-epigalocatechin on Fusobacterium nucleatum, Prevotella intermedia and Porphyromonas gingivalis

  • 박재윤 (조선대학교 의과학전문대학원 생화학 분자생물학교실) ;
  • 김화숙 (전남과학대학 치위생과) ;
  • 국중기 (조선대학교 치의학전문대학원 구강생화학교실)
  • Park, Jae-Yoon (Department of Biochemistry and Molecular Biology, Medical School, Chosun University) ;
  • Kim, Hwa-Sook (Department of Dental Hygiene, Chunnam Techno College) ;
  • Kook, Joong-Ki (Department of Oral Biochemistry, School of Dentistry, Chosun University)
  • 투고 : 2010.04.06
  • 심사 : 2010.06.18
  • 발행 : 2010.06.30
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초록

녹차의 폴리페놀류 중 수용성인 (-)-epigalocatechin을 이용하여 치주질환 원인균에 대한 항세균 작용을 조사한 결과 다음과 같은 결론을 얻었다. 1. (-)-epigalocatechin은 주요한 치주질환 원인균들인 F. nucleatum, P. intermedia 및 P. gingivalis에 대한 최소성장억제농도가 0.625 mg/ml 이하로 항균 작용이 높은 것으로 나타났다. 2. Time-kill 분석법을 실시한 결과 (-)-epigalocatechin은 F. nucleatum, P. intermedia 및 P. gingivalis에 살균작용이 있는 것으로 나타났다. 이상의 결과에서 (-)-epigalocatechin은 치주질환의 예방 및 치주 치료 후 예후를 증진시킬 수 있는 가글린 및 치약 등의 구강위생용품 개발에 이용할 수 있을 것으로 생각된다.

The aim of this study was to investigate the antimicrobial effect of (-)-epigalocatechin on Fusobacterium nucleatum, Prevotella intermedia, and Porphyromonas gingivalis. To test the antimicrobial effect of (-)-epigalocatechin, the minimum inhibitory concentration (MIC) of against 4 strains of F. nucleatum, 2 strains of P. intermedia, and 2 strains of P. gingivalis was measured by broth dilution method. Time-kill curves were assessed for susceptible bacteria, testing $0{\times}MIC$ (control group), $0.5{\times}MIC$, $1{\times}MIC$, and $2{\times}MIC$ for (-)-epigalocatechin, by counting viable bacteria after 3, 90, 180, 360, 720, 1440 minutes. The MIC of (-)-epigalocatechin was 0.312-0.625, 0.625, and 0.625 mg/ml on the strains of F. nucleatum, P. intermedia, and P. gingivalis, respectively. Time-kill curves demonstrated (-)-epigalocatechin had bactericidal activity on P. intermedia ATCC $25611^T$, P. gingival is ATCC 53978, and F. nucleatum subsp. fusiforme ATCC $51190^T$. The results suggest that (-)-epigalocatechin can be useful in developing the oral hygiene product such as tooth past and gargling solution for the prevention of periodontal diseases.

키워드

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