Journal of International Academy of Physical Therapy Research (국제물리치료학회지)

International Academy of Physical Therapy Research (국제물리치료연구학회)
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The Effects of Needle Electrode Electrical Stimulation on Cellular Necrosis Blocking the Forebrain after Induction of Ischemia

  • Received : 2009.12.16
  • Accepted : 2010.01.20
  • Published : 2010.03.31
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Abstract

This study was performed to investigate the effects of Needle Electrode Electrical Stimulation(NEES) on ischemia-induced cerebrovascular accidents. After obstruction and reperfusion of arteries in white mice, the amounts of necrosis and inflammation related substances Bax, IL-6, Caspase-3, and COX-2 were measured in neurons of the fore-brain. The following results were obtained. This study used 21 male specific pathogen free(SPF) SD rats, 8 weeks of age and approximately 300g in weight. Each exposed artery was completely occluded with non-absorbent suture thread and kept in that state for 5 minutes. The sutures were then removed to allow reperfusion of blood. Test group is control group(common carotid artery occlusion models), a GI(underwent common carotid artery occlusion), and NEES(underwent NEES after artery occlusion). The GI and NEES groups were given 12, 24, or 48 hours of reperfusion before NEES. NEES device(PG6, ITO, Japan, 9V, current, 2Hz) was used to stimulate the bilateral acupoint ST36 of the SD rats for 30 minutes while they were sedated with 3% isoflurane. An immuno-histochemistry test was done on the forebrains of the GI induced rats. Both Bax and Caspase-3 immuno-reactive cells, related to apoptosis, were greater in the GI than the NEES group. Cox-2 and IL-6 immuno-reactive cells, related to inflammation, were greater in the GI and NEES groups than the control group. We can expect that applying NEES after ischemic CVA is effective for preventing brain cells from being destroyed. And we can conclude NEES should be applyed on early stage of ischemic CVA.

Keywords

References

  1. Hinkle L, McCaug CD, Robinson KR. The direction of growth of differentiating neurons and myoblasts from frog embryos in an applied electric field. J Physol 1981; 314: 121-135. https://doi.org/10.1113/jphysiol.1981.sp013695
  2. Kerns JM, Fakhouri AJ and Weinrib HP. Electrical stimulation of nerve regerneration in the rat the early effects evaluated by a vibrating probe and elctro- microscopy. Neuroscience 1991; 40: 93-107. https://doi.org/10.1016/0306-4522(91)90177-P
  3. Kerns JM, Lucchinetti C. Electrical field effects on crushed nerve regeneration. Exp Neurol 1992; 117: 71-80. https://doi.org/10.1016/0014-4886(92)90112-4
  4. Wu MT, Hsieh JC, Xiong J, Yang CF, Pan HB, Chen YCI, Tsai GC, Bruce Rosen R, Kenneth K, Kwong. Central Nervous Pathway for Acupunture Stimulation Localization of Procession with Functional MR Imaging of the Brain- Preliminary Experience. Radiology 1999; 212: 133-141. https://doi.org/10.1148/radiology.212.1.r99jl04133
  5. Lunderberg T. Does acupuncture work. IASP 1996; 4(3): 1-4.
  6. Du C, Hu R, Csernansky CA, Hsu CY and Choi DW. Very delayed infarction after mild focal cerebral ischemia a role for apoptosis. J Cereb Blood Flow Metab 1996; 16: 195-201. https://doi.org/10.1097/00004647-199603000-00003
  7. Zheng Z, Zhao H, Steinberg GK, Yenari MA. Cellular and molecular events underlying ischemia-induced neuronal apoptosis. Drug News Perspect 2003; 16: 497-503. https://doi.org/10.1358/dnp.2003.16.8.829348
  8. Hara AT, Iwai, Niwa M. Immunohistochemical detection of BAX and Bcl-2 proteins in gerbil hppocampus followiong transient forebrain ischemia. Brain Res 1996; 711: 249-253. https://doi.org/10.1016/0006-8993(95)01436-5
  9. Gibson ME, Han BH, Choi J, Knudson CM, Korsmeyer SJ, Parsadanian M, Holtzman DM. BAX contributes to apoptotic-like death following meonatal hypoxia-ischemia evidence for distinct apoptosis pathways. Mol Med 2001; 7(9): 644-655.
  10. Rao FV, Peel A, Logbinova A, Rio GD, Hermel E, Yokota T, Goldsmith PC, Ellerby LM , Ellerby HM and Bredesen DE. Coupling endoplasmic reticulum stress to the cell death program role of the ER chaperone GRP78. FEBS letters 2002; 514: 122-128. https://doi.org/10.1016/S0014-5793(02)02289-5
  11. Chen J, Nagayama T, Jin K, Stetler RA, Zhu RL, Graham SH and Simon RP. Induction of caspase-3 like protease may mediate delayed neuronal death in the hippocampus after transient cerebral ischemia. J Neurosci 1998; 18: 4914-1428.
  12. Graham SH, Chen J. Programmed cell death in cerebral ischemia. J Cereb Blood Flow Metab 2001; 21: 99-109. https://doi.org/10.1097/00004647-200102000-00001
  13. Saleh A, Srinivasula SM, Acharya S, Fishel R, Alnemri ES. Cytochrome c and dATP-mediated oligomrization of apaf-1 is a prerequisite for procaspase-9 activation. J Biol Chem 1999; 274: 17941-17945. https://doi.org/10.1074/jbc.274.25.17941
  14. Hitoshi, Yoshida, Takeshi, Kubota, James M, Kruegar. A cyclooxygenase-2 inhibitor attenuates spontaneous and TNF-$\alpha$-induced non-rapid eye movement sleep in rabbits. Am J Physiol Regul Integr Comp Physiol 2003; 285: R99-R109. https://doi.org/10.1152/ajpregu.00609.2002
  15. Weisz G, Lavy AY, Adir, YD, Melamed S and Rubin S. Eidelman and Pollack. Modification of in vivo and in vitro TNF-$\alpha$ IL-1 and IL-6 seretion by circulating monocytes during hyperbaric oxygen treatment in patients with perianal Crohn's disease. J Clin Immunol 1997; 17: 154-158. https://doi.org/10.1023/A:1027378532003
  16. Flack J, Kimura Y, Engelman RM and Das DK. Preconditioning the heart by repeated K Frei, UV Malipiero, TP Leist, RM Zinkernage, ME Schwab and A Frontana. Production of B cell stimulatory factor-2 and interferon $\gamma$ in the central nervous system during viral meningitis and encephalitis. J Exp Med 1988; 168: 449-453. https://doi.org/10.1084/jem.168.1.449
  17. Masada T, Hua Y, Xi G, Ennis SR, Keep RF. Attenuation of ischemic brain edema Mi-Hyeon Jang. Acupuncture suppresses ischemia-induced increase in c-Fos expression and apoptosis in the hippocampal CA1 region in gerbils. Neuroscence Letters 2003; 347: 5-8. https://doi.org/10.1016/S0304-3940(03)00512-3
  18. Andjelkovic AV, Stamatovic SM and Keep RF. The protective effects of preconditioning on cerebral endothelial cells in vitro. J Cereb Blood Flow Metab 2003; 23: 1348-1355. https://doi.org/10.1097/01.WCB.0000091762.61714.FE
  19. Nicholson DW. Mechanisms of apoptotic control. Nature 2000; 407: 810-816. https://doi.org/10.1038/35037747
  20. Nicoletti VG, Stella AM. Role of PARP under stress conditions : cell death or protection. Neurochemical Res 2003; 28187-28194.