Journal of dental hygiene science (치위생과학회지)

The Korean Society of Dental Hygiene Science (한국치위생과학회)
권호 표지

Antioxidative Activities and Antiproliferation Effects on Oral Carcinoma KB Cell of the Brazilin from Caesalpinia sappan L.

소목(Caesalpinia sappan L.)에서 분리한 Brazilin의 항산화 활성과 구강상피암 KB 세포주의 증식억제효과

  • Jeon, Mi-Ae (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Kwon, Hyun-Jung (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Kim, Yong-Hyun (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Han, Kook-Il (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Chung, Ha-Na (Dept. of Biology, College of Natural Science, Soonchunhyang University) ;
  • Chung, Sung-Kyun (Dept. of Dental hygiene, Shinheung College) ;
  • Bang, In Seok (Dept. of Biological Science and the Research Institute for Basic Sciences, Hoseo University) ;
  • Han, Man-Deuk (Dept. of Biology, College of Natural Science, Soonchunhyang University)
  • 전미애 (순천향대학교 생명과학과) ;
  • 권현정 (순천향대학교 생명과학과) ;
  • 김용현 (순천향대학교 생명과학과) ;
  • 한국일 (순천향대학교 생명과학과) ;
  • 정하나 (순천향대학교 생명과학과) ;
  • 정성균 (신흥대학 치위생과) ;
  • 방인석 (호서대학교 생명과학과) ;
  • 한만덕 (순천향대학교 생명과학과)
  • Received : 2010.09.10
  • Accepted : 2010.10.18
  • Published : 2010.10.30
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Abstract

Caesalpinia sappan L. has long been commonly used in oriental folk medicines and as dyes materials. To investigate the antioxidative activities and antiproliferation effects of brazilin from C. sappan heart wood, the MeOH soluble extract was successively fractionated by using hexane, $CHCl_{3}$, EtOAc, BuOH, MeOH, and $H_{2}O$. In these fractions, we were purified brazilin from EtOAc fraction which partitioned to 3.94% of the highest yields. The effects of brazilin and the extracts on human oral carcinoma cells (KB) by MTT assay and their antioxidant activities by DPPH, TCA assay and Fenton reaction were tested. The results showed that the brazilin could inhibits the proliferation of KB cells and obviously decreased the production of nitric oxide of the cells. When the concentration of the brazilin reached to $100\;{\mu}g/ml$, the inhibition percentage of the cell growth was about 60%. In assay on antioxidant activities, The results showed that brazilin exhibit the highest capacity of DPPH free radical scavenging effects among tested extracts. When the concentration of brazilin reached to 1 mg/ml, the lipid peroxide inhibition and radical inhibition activities were determined to be 65.0% and 85.8%, respectively. These results are suggest that brazilin have stronger antiproliferation effect on KB cell and antioxidant properties.

본 연구는 brazilin의 항산화 효과 및 구강암세포에 대한 암세포 증식억제 효과를 확인하고자 활성산소 소거능력을 측정하고, KB cell에 brazilin을 처리하여 세포 성장억제 등을 측정하였다. 시행된 실험결과를 분석한 결과 다음과 같은 결론을 얻었다. 1. Brazilin은 소목의 다른 유기용매 추출물에 비하여 최저 농도에서도 9%의 전자공여능 소거효과를 보이고 최고농도에서 90%의 소거효과를 나타내었다. 또한 리놀레산 자동 산화 방지 효과를 측정한 결과 26%에서 66%까지 억제하였다. Hydroxyl radical 소거효과 측정 결과 1 mg/ml에서 86%의 소거효과를 나타내었고, 0.001 mg/ml에서도 5%의 소거효과를 나타내었다. 2. Brazilin은 KB cell의 성장을 1시간 처리했을 경우 최저 5%에서 최대 23%까지 억제하였으며, 24시간 처리했을 경우 5%에서 최대 64%까지 억제하였고, 48시간 처리 후에는 최소 3%, 최대 79%까지 억제하였다. 3. KB cell에 LPS 처리 시 NO를 $250\;{\mu}M$ 이상 생성시켰으며, 세포증식률을 231% 증가시켰고, LPS를 처리한 KB cell에 brazilin을 처리한 결과 세포의 증식률을 -7%에서 78%까지 감소시켰다. 또한 KB cell에 $H_2O_2$를 처리한 결과 세포증식률이 감소되었으며, brazilin을 첨가하였을 때 $H_2O_2$에 대한 소거작용으로 인해 생존 억제율이 72%에서 58%로 감소하였다.

Keywords

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