식물분류학회지 (Korean Journal of Plant Taxonomy)

  • 제40권2호
  • /
  • Pages.118-129
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  • 2010
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  • 1225-8318(pISSN)
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  • 2466-1546(eISSN)
한국식물분류학회 (The Korean Society of Plant Taxonomists)
권호 표지

RAPD 자료에 근거한 태백제비꽃군의 분류학적 연구

Taxonomic study of Viola albida complex based on RAPD data

  • 구자춘 (전북대학교 과학교육학부) ;
  • 탁효진 (전북대학교 과학교육학부) ;
  • 황성수 (전북대학교 과학교육학부)
  • Koo, Ja Choon (Division of Science Education, Chonbuk National University) ;
  • Tak, Hyo Jin (Division of Science Education, Chonbuk National University) ;
  • Whang, Sung Soo (Division of Science Education, Chonbuk National University)
  • 투고 : 2010.05.25
  • 심사 : 2010.06.21
  • 발행 : 2010.06.30
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초록

태백제비꽃군내 태백제비꽃, 단풍제비꽃 그리고 남산제비꽃의 대표적인 개체들을 대상으로 RAPD 방법을 사용하여 분류학적 연구를 시도하였다. 7개의 OTU와 68개의 primers를 사용하여 얻은 증폭된 자위(amplified loci)의 숫자는 모두 476개 이었다. Nei의 유전적 비유사도 지수가 남산제비꽃 개체들에서는 0.051로, 태백제비꽃 개체들은 0.118-0.171로 비교적 낮게 나타난 반면, 단풍제비꽃 개체들은 0.348로 나타나서 상기의 두 종보다 높게 나타났다. 한편, 태백제비꽃 개체들과 단풍제비꽃 개체들, 태백제비꽃 개체들과 남산제비꽃개체들 그리고 단풍제비꽃 개체들과 남산제비꽃 개체들 사이의 유전적 비유사성은 전체적으로 0.214-0.463의 범주로 나타나서 분류군간 뚜렷한 특징을 나타내지 않았다. 유집분석에서 조사된 개체들의 유사도는 비교적 높게 나타나서 종내 복합체 설정을 지지하였으며, 두 개의 집단으로 유집되었다. 그러나 단풍제비꽃 개체들은 태백제비꽃 또는 남산제비꽃 집단에 각각 유집되어 형태적 변이와 함께 유전적 다양성도 높은 것으로 확인되었다.

A taxonomic study of Viola albida complex, containing the representative individuals of three taxa, V. albida var. albida, V. albida var. chaerophylloides, and V. albida var. takahashii, was done based on RAPD data. The amplified loci were 476 in total; obtained with 68 universal primers on seven OTUs. Nei's genetic dissimilarity appeared relatively low within individuals of V. albida var. albida and V. albida var. chaerophylloides (0.118-0.171 and 0.051 respectively), however, it was higher in individuals of V. albida var. takahashii (0.348). On the other hand, there is no specific trend in terms of genetic dissimilartiy among taxa, such as between individuals of V. albida var. albida and V. albida var. takahashii, between those of V. albida var. albida and V. albida var. chaerophylloides, and between those of V. albida var. albida and V. albida var. takahashii. The similarity of OTUs studied is high in clustering analysis, so that this result is compatible with the establishment of this complex. All OTUs are clustered within two groups. The individuals of V. albida var. takahashii, however, are clustered both to the group of V. albida var. albida and to the group of V. albida var. chaerophylloides, meaning that the genetic difference is high which would be commensurate with their morphological variations.

키워드

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