냉성어혈약침이 외상성 뇌손상 흰쥐의 신경학적 회복과 BAX, BCL-2의 발현에 미치는 영향

Effects of Cool Pharmacopuncture for Static Blood into Hyolhae(Sp10 ) on Neurological Recoverment and BAX, BCL-2 Expression in the Experimental Traumatic Brain Injury Rats

  • 조주현 (동신대학교 한의과대학 한방재활의학과교실) ;
  • 최진봉 (동신대학교 한의과대학 한방재활의학과교실)
  • Cho, Joo-Hyun (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Shin University) ;
  • Choi, Jin-Bong (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Shin University)
  • 투고 : 2009.01.06
  • 심사 : 2010.01.16
  • 발행 : 2010.01.31

초록

Objectives : Cool Pharmacopuncture for static blood is a famous pharmacopuncture treatment that treated disease caused by static blood. Hyolhae(Sp10) is also a famous point of acupuncture that treated that. This study was designed to evaluate the effects of Cool Pharmacopuncture for static blood into Hyolhae(Sp10) on BAX and BCL-2 expression in the experimental traumatic brain injury(TBI) rats. Methods : Male rats were divided into 3 groups. I was no treatment after TBI. II was treatment with needle-prick acupuncture after TBI. III was treatment with Cool Pharmacopuncture for static blood into Hyolhae(Sp10) after TBI. The author carried out neurological motor behavioral test, histological assessment test. Neurological motor behavior tests consist of rotarod test, beam-walking test and postural reflex test. In the histological assessment test, BAX and BCL-2 expression, hematoxylin & eosin staining were experimented. Results : In neurological motor behavior tests, motor and cognitive function recovery was significantly increased in the II, III as compared with I (p<0.05). Especially III was significantly increased as compared with II (p<0.05). BAX expression was significantly decresed in order of the III, II, I after 7 and 14 days later. BCL-2 expression was investigated in the III, II as compared with I. Especially Most incresed expression was experimented in the III. Conclusions : According to the above results, Cool Pharmacopuncture for static blood can inhibit apoptosis of cells after TBI in rats by contol of BAX and BCL expression.

키워드

참고문헌

  1. Zhang L, Yang KH, King AI. Biomechanics of neurotrauma. Neurol Res. 2001;23(2-3):144-56. https://doi.org/10.1179/016164101101198488
  2. 김진호, 한태륜. 재활의학. 서울:군자출판사. 2006:366-7.
  3. 안영기. 경혈학총서. 서울:성보사. 1995:236-7.
  4. 임종국. 침구치료학. 서울:집문당. 1983:320.
  5. 大韓藥鍼學會. 약침요법총론. 서울:대한약침학회. 1999:314
  6. 지용철. 흰쥐의 실험적 외상성 뇌손상에서 Ginseng Total Saponins의 신경보호 작용. 중앙대학교 대학원 박사학위논문. 2005.
  7. 김정길. 홍삼투여가 흰쥐 외상성 뇌손상의 Bax 및 Bcl-2 발현에 미치는 영향. 경희대학교 대학원 석사학위논문. 2007.
  8. 나건호, 윤대환, 나창수, 채우석. 풍지(風池) (GB20)의 천마약침(天麻藥鍼)이 Intraluminal Filament 삽입술에 의하여 유발된 백서(白鼠)의 뇌허혈에 미치는 영향. 대한침구학회지. 2008;25(1):1-14.
  9. 한상균, 이병렬. 당귀약침의 혈해 자입이 Intraluminal Filament 삽입술에 의해 유발된 백서의 허혈성 뇌손상에 미치는 영향. 대한침구학회지. 2004;21(2):1-20.
  10. Marmarou A, Foda MA, van den Brink W, Campbell J, Kita H, Demetriadou K. A new model of diffuse brain injury in rats. Part I: Pathophysiology and biomechanics. J Neurosurg. 1994;80(2):291-300. https://doi.org/10.3171/jns.1994.80.2.0291
  11. Hamm RJ, Pike BR, O'dell DM, Lyeth BG, Jenkins LW. The rotarod test: An evqluation of its effectiveness in assessing motor deficits following traumatic brain injury. J Neurotrauma. 1994;11(2):187-96. https://doi.org/10.1089/neu.1994.11.187
  12. Feeney DM, Gonzalez A, Law WA. Amphetamine, haloperidol and experience interact to affect rate of recovery after motor cortex injury. Science. 1982;218:855-7.
  13. Goldstein LB, Davis JN. Beam-walking in rats: Studies towards developing an animal model of functional recovery after brain injury. J Neurosci Methods. 1990;31:101-7. https://doi.org/10.1016/0165-0270(90)90154-8
  14. 최석민, 석종식, 민병국, 황성남, 김영백, 김재현. 개량형 피질 충격 모델의 개발.대한신경외과학회지. 2002;32(5):458-62.
  15. Royo NC, Schouten JW, Fulp CT, Shimizu S, Marklund N, Graham DI, McIntosh TK. From cell death to neuronal regeneration: building a new brain after traumatic brain injury. J Neuropathol Exp Neurol. 2003;62:801-11. https://doi.org/10.1093/jnen/62.8.801
  16. 한국생화학분자생물학회. Poster Session: Simvastatin induces apoptosis through Bax translocation to mitochondria in MethA fibrosarcoma cells. 춘계학술대회집. 2006.
  17. 박지현, 김세련, 송은섭, 임문환, 이병익, 이우영. 태아 성장 발육장애 또는 전자간증 태반에서의 세포고사: Bcl-2, Bax 및 p53 단백 발현에 관한 연구. 대한산부인과학회지. 2005;48(4):891-900.
  18. Hausmann R, Biermann T, Wiest I, Tubel J, Betz P, Neuronal apoptosis following human brain injury. Int J Legal Med. 2004;118(1):32-6. https://doi.org/10.1007/s00414-003-0413-4
  19. Colicos MA, Dash PK. Apoptotic morphology of dentate gyrus granule cells following experimental cortical impact injury in rats: possible role in spatial memory deficits. Brain Res. 1996;739:120-31. https://doi.org/10.1016/S0006-8993(96)00824-4
  20. Kaya SS. Mahmood A, Li Y, Yavuz E, Goksel M, Chopp M. Apoptosis and expression of p53 response proteins and cyclin D1after cortical impact in rat brain. Brain Res. 1999;818:23-33. https://doi.org/10.1016/S0006-8993(98)01204-9
  21. 김영화, 김영일, 이현. 음릉천 혈해의 자침 부작용치료에 대한 증례보고. 대전대학교 한의학연구원. 2006:47-52.
  22. 김상희, 손인길, 엄태식. 혈해.격수혈의 혈성 및 구 횟수와 주치증에 대한 문헌적 고찰. 대한침구학회지. 1994;11(1):241-52.
  23. 大韓藥鍼學會編. 약침요법 시술지침서. 서울:대한약침학회. 2000:161-2, 284, 323.
  24. 大韓藥鍼學會編譯. 약침제제와 임상응용(1). 서울:대한약침학회. 1997:1-5.
  25. 임상본초학. 서울:영림사. 2000:242, 372, 401-5, 533.
  26. 허준. 동의보감. 서울:대성문화사. 1992:55, 466.
  27. Nicholson DW. Mechanisms of apoptotic control. Nature. 2000;407:810-6. https://doi.org/10.1038/35037747
  28. Kannan, K, Jain, S. K. Oxidative stress and apoptosis. Pathophysiology. 2000;7(27):153-63. https://doi.org/10.1016/S0928-4680(00)00053-5
  29. Srinivasula, S. M, Ahmad, M, Fernandes-Alnemri, T. & Alnemri, E. S. Autoactivation of procaspase-9 by Apf-1-mediated ologomerization. Molecular Cell. 1998;1:949-57. https://doi.org/10.1016/S1097-2765(00)80095-7
  30. Fadeel, B., Zhivotosky, B. & Orrenius, S. All along the watchtower: on the regulation of apoptosis regulators. The FASEB journal. 1999;13:1647-57.
  31. Krajewski S, Krajewska M, Shabaik A, Miyashita T, Wang HG, Reed JC. Immunohistochemical determination of in vivo distribution of Bax, a dominant inhibitior fo Bcl-2. Am J Pathol. 1994;145(6):1323-36.
  32. Clark RSB, Kochanek PM, Adelson PD, Bell MJ, Carcillo JA, Chen M, Wisniewski SR, Janesko K, Whalen MJ, Graham SH. Increases in bcl-2 protein in cerebrospinal fluid and evidence for programmed cell death in infants and children after severe traumatic brain injury. J Pediatr. 2000;137(2):197-204. https://doi.org/10.1067/mpd.2000.106903
  33. ONG YL, McMullin MF, Bailie KEM, Lappin TRJ, Jones FG, Irvine AE. Hing bax expression is a good prognostic indicator in acute myeloid leukemia. Br J Haematol. 2000;111:182-9. https://doi.org/10.1046/j.1365-2141.2000.02315.x