Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
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Preparation and Characterization of Bovine Serum Albumin-loaded Cationic Liposomes: Effect of Hydration Phase

  • Park, Se-Jin (College of Pharmacy, Chungnam National University) ;
  • Jeong, Ui-Hyeon (College of Pharmacy, Chungnam National University) ;
  • Lee, Ji-Woo (College of Pharmacy, Chungnam National University) ;
  • Park, Jeong-Sook (College of Pharmacy, Chungnam National University)
  • Received : 2010.12.01
  • Accepted : 2010.12.17
  • Published : 2010.12.20
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Abstract

Although liposomes have been applied as drug delivery systems in various fields, the usage was limited due to the low encapsulation efficiency compared to other carrier systems. Here, cationic liposomes were prepared by mixing 1,2-dioleoyl-3-trimethylammoniopropane (DOTAP) as a cationic lipid, 1,2-dioleoyl-sn-glycerol-phosphoethanolamine (DOPE) and cholesterol (CH), and the liposomes were hydrated by varying the aqueous phases such as phosphate-buffered saline (PBS), 5% dextrose, and 10% sucrose in order to improve the encapsulation efficiency of bovine serum albumin (BSA). The particle size and zeta potential were determined by dynamic light scattering method and in vitro release patterns were investigated by spectrophotometry. Particle size and zeta potential of liposomes were varied depending on the ratio of DOTAP/DOPE/CH in range of 270-350 nm and 0.8-9.7 mV, respectively. Moreover, the addition of polyethylene glycol (PEG) improved the encapsulation efficiency from 37% to 43% as well as reduced particle sizes of liposomes while the liposomes were hydrated in PBS. When the liposomes were hydrated with 10% sucrose, the encapsulation efficiency of BSA was higher than any other groups. Whereas PBS was used as hydration solution, lower encapsulation efficiency was obtained compared with other groups. More than 60% of BSA was released from the liposomes hydrated with 10% sucrose; thereafter another 20% of BSA was released. Therefore, release pattern of BSA from cationic liposomes was extended release in this study. From the results, cationic liposomes dispersed in 10% sucrose would be potential carrier with high encapsulation efficiency.

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References

  1. Blume, G., Cevc, G., 1990. Liposomes for the sustained drug release in vivo. Biochim. Biophys. Acta 1029, 91-97. https://doi.org/10.1016/0005-2736(90)90440-Y
  2. Crowe, J.H., Tsvetkova, N.M., Oliver, A.E., Leidy, C., Ricker, J., Crowe, L.M., 2006. Stabilization of liposomes by freeze-drying: lessons from nature. In: Gregoriadis, G. (Ed.), Liposome Technology, Vol. I: Liposome Preparation and Related Techniques, 3rd Ed., Informa Healthcare, USA.
  3. Debs, R.J., Fuchs, H.J., Philip, R., Brunette, E.N., Duzgunes, N., Shellito, J.E., Liggitt, D., Patton, J.R., 1990. Immunomodulatory and toxic effects of free and liposome-encapsulated tumor necrosis factor alpha in rats. Cancer Res. 50, 375-380.
  4. Gabizon, A., Martin, F., 1997. Polyethylene glycol-coated (pegylated) liposomal doxorubicin. Rationale for use in solid tumors. Drugs 54, 15-21.
  5. Jeong, U.H., Jung, J.H., Davaa, E., Park, S.J., Myung, C.S., Park, J.S., 2009. Effect of drug loading on the physicochemical properties and stability of cationic lipid-based plasmid DNA complexes. J. Kor. Pharm. Sci. 39, 339-343. https://doi.org/10.4333/KPS.2009.39.5.339
  6. Katayama, K., Kato, Y., Onishi, H., Nagai, T., Machida, Y., 2003. Double liposomes: hypoglycemic effects of liposomal insulin on normal rats. Drug Dev. Ind. Pharm. 29, 725-731. https://doi.org/10.1081/DDC-120021771
  7. Kim, J.K., Choi, S.H., Kim, C.O., Park, J.S., Ahn, W.S., Kim, C.K., 2003. Enhancement of polyethylene glycol (PEG)-modified cationic liposome-mediated gene deliveries: effects on serum stability and transfection efficiency. J. Pharm. Pharmacol. 55, 453-460. https://doi.org/10.1211/002235702928
  8. Kim, J.Y., Kim, J.K., Park, J.S., Byun, Y., Kim, C.K., 2009. The use of PEGylated liposomes to prolong circulation lifetimes of tissue plasminogen activator. Biomaterials 30, 5751-5756. https://doi.org/10.1016/j.biomaterials.2009.07.021
  9. Lee, K.Y., Yuk, S.H., 2007. Polymeric protein delivery systems. Prog. Polym. Sci. 32, 669-697. https://doi.org/10.1016/j.progpolymsci.2007.04.001
  10. Lian, T., Ho, R.J., 2001. Trends and developments in liposome drug delivery systems. J. Pharm. Sci. 90, 667-680. https://doi.org/10.1002/jps.1023
  11. Manosroi, A., Khanrin, P., Werner, R.G., Gotz, F., Manosroi, W., Manosroi, J., 2010. Entrapment enhancement of pepetide drugs in niosomes. J. Microencapsul. 27, 272-280. https://doi.org/10.3109/02652040903131293
  12. Meyenburg, S., Lilie, H., Panzner, S., Rudolph, R., 2000. Fibrin encapsulated liposomes as protein delivery system: Studies on the in vitro release behavior. J. Control. Release 69, 159-168. https://doi.org/10.1016/S0168-3659(00)00295-9
  13. Mokhtar, M., Sammour, O.A., Hammad, M.A., Megrab, N.A., 2008. Effect of some formulation parameters on flurbiprofen encapsulation and release rates of niosomes prepared from proniosomes. Int. J. Pharm. 361, 104-111. https://doi.org/10.1016/j.ijpharm.2008.05.031
  14. van Winden, E.C., 2003. Freeze-drying of liposomes: theory and practice. Meth. Enzymol. 367, 99-110. https://doi.org/10.1016/S0076-6879(03)67008-4
  15. Vandana, M., Sahoo, S.K., 2009. Optimization of physicochemical parameters influencing the fabrication of protein-loaded chitosan nanoparticles. Nanomedicine (Lond) 4, 773-785. https://doi.org/10.2217/nnm.09.54
  16. Wang, W., 2005. Protein aggregation and its inhibition in biopharmaceutics. Int. J. Pharm. 289, 1-30. https://doi.org/10.1016/j.ijpharm.2004.11.014
  17. Werle, M., Bernkop-Schnurch, A., 2006. Strategies to improve plasma half life time of peptide and protein drugs. Amino Acids 30, 351-367. https://doi.org/10.1007/s00726-005-0289-3