한국어병학회지 (Journal of fish pathology)

  • 제23권1호
  • /
  • Pages.9-16
  • /
  • 2010
  • /
  • 1226-0819(pISSN)
  • /
  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

Influence of temperature shifts on the kinetics of the specific antibody secreting cells in the olive flounder Paralichthys olivaceus

  • Yoon, So-Hye (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Hong, Su-Hee (Faculty of Marine Bioscience and Technology, Department of Chemistry, Gangneung-Wonju National University) ;
  • Jeong, Joon-Bum (School of Marine Biomedical Sciences, College of Ocean Science & Marine and Environmental Research Institute, Jeju National University) ;
  • Jeong, Hyun-Do (Department of Aquatic Life Medicine, Pukyong National University)
  • 투고 : 2010.03.09
  • 심사 : 2010.04.21
  • 발행 : 2010.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

We determined the effects of temperature shifts on the kinetics of the numbers of antibody-secreting cell (ASC) in the olive flounder Paralichthys olivaceus immunised with formalin-killed Edwardsiella tarda. When fish that were acclimated to $22^{\circ}C$ and immunised at that temperature were transferred to a lower temperature ($12^{\circ}C$) at a various times (immediately, 1, 2 or 4 weeks) after immunisation, both further differentiation of B cells and secretion of antibody from the ASC developed at $22^{\circ}C$ were suppressed at $12^{\circ}C$. However, in the converse experiment ($12^{\circ}C$ to $22^{\circ}C$), the magnitude of the humoral immune response was recovered independent of the time of the transfer after immunisation at low temperature, even though the peak levels of each transferred group did not reach the level found in $22^{\circ}C$ control group. The results were confirmed by counting the number of specific antibody secreting cells (SASC) in the spleen. This study provides the evidences of the immune reaction that the potential for antibody production in B cells of flounder, the most important species in aquatic industry of Korea, immunized at high temperature is suppressed by subsequent exposure to low temperature and that low temperature-induced humoral immuno suppression can be reversed by exposure to a higher temperature.

키워드

참고문헌

  1. Aaltonen, T.M,, Jokinen, E.I. and Valtonen, E.T.:Antibody synthesis in roach (Rutilus rutilus); analysis of antibody secreting cells in lymphoid organ with ELISPOT-assay. Fish Shellfish Immunol., 4:129-140, 1994. https://doi.org/10.1006/fsim.1994.1011
  2. Attia-El-Hili, H., El-Gaaid, A., Troutaud, D. and Deschaux, P.: Study of reared sea bream antibody response to a sheep red blood cells injection. Ichtyophsiol. Acta., 15:7-20, 1992.
  3. Bagni, M., Romano, N., Finoia, M.G., Abelli, L., Scapigliati, G., Tiscar, P.G., Sarti, M. and Marino, G.: Short- and long-term effects of a dietary yeast beta-glucan (Macrogard) and alginic acid (Ergosan) preparation on immune response in sea bass (Dicentrarchus labrax). Fish Shellfish Immunol., 18:311-325, 2005. https://doi.org/10.1016/j.fsi.2004.08.003
  4. Bly, J.E. and Clem, L.W.: Temperature and teleost immune functions. Fish Shellfish Immunol., 2:159-171, 1992. https://doi.org/10.1016/S1050-4648(05)80056-7
  5. Bromage, E.S., Kaattari, I.M., Zwollo, P. and Kaattari, S.K.: Plasmablast and plasma cell production and distribution in trout immune tissues. J. Immunol., 173:7317-7323, 2004. https://doi.org/10.4049/jimmunol.173.12.7317
  6. Corbel, M.J.: The immune response in fish: a review. J. Fish Biol., 7: 539-563, 1975. https://doi.org/10.1111/j.1095-8649.1975.tb04630.x
  7. Clem, L.W., Faulmann, E., Miller, N.W., Ellsaesser, C., Lobb, C.J. and Cuchens, M.A.: Temperature-mediated processes in teleost immunity : differential effects of in vitro and in vivo temperatures on mitogenic responses of channel catfish lymphocytes. Dev. Comp. Immunol., 8:313-322, 1984. https://doi.org/10.1016/0145-305X(84)90038-7
  8. Davidson, G.A., Ellis, A.E. and Secombes, C.J.: An ELISPOT assay for the quantification of specific antibody secreting cells to Aeromonas salmonicida in rainbow trout, Onchorhynchus mykiss (Walbaum). J. Fish Dis., 15:85-89, 1992. https://doi.org/10.1111/j.1365-2761.1992.tb00640.x
  9. Dezin, N and Staak, C: Fish Immunoglobulin - A Sero-Diagnostician's Perspective. Bull. Eur. Ass. Fish Pathol., 20(2):60-64, 2000.
  10. Ellsaesser, C.F., Bly, J.E. and Clem, L.W.: Phylogeny of lymphocyte heterogeneity: the thymus of the channel catfish. Dev. Comp. Immunol., 12:787-799, 1998.
  11. Hawley, L. and Garver, K.: Stability of viral hemorrhagic septicemia virus (VHS) Ifreshwater and seawater at various temperatures. Dis. Aqu. Org., 82:171-178, 2008. https://doi.org/10.3354/dao01998
  12. Hurubec, T.C., Robertson, J.L., Smith, S.A. and Tinker, M.K.: The effect of temperature and water quality on antibody response to Aeromonas salmonicida in sunshine bass (Morone chrysops X Morone saxatilis). Vet. Immunol. Immunopathol., 50:157-166, 1996. https://doi.org/10.1016/0165-2427(95)05491-X
  13. King, V.W., Buckley, J.L. and Berlinsky, L.D.: Effect of acclimation temperature on the acute stress response in juvenile Atlantic cod, Gadus morhua L., and haddock, Melanogrammus aeglefinus L. Aquaculture Research 37:1685-1693, 2006. https://doi.org/10.1111/j.1365-2109.2006.01623.x
  14. King, V.W. and Berlinsky, D.L.: Whole-body corticosteroid and plasma cortisol concentrations in larval and juvenile Atlantic cod Gadus morhua L. following an acute stressor. Aquaculture Research 37:1282-1289, 2006. https://doi.org/10.1111/j.1365-2109.2006.01558.x
  15. Koskela, J., Rahkonen, R., Pasternack, M. and Knuutinen, H. : Effect of immunization with two commercial vaccines on feed intake, growth, and lysozyme activity in European white fish (Coregonus lavaretus L.). Aquaculture, 234:41-50, 2004. https://doi.org/10.1016/j.aquaculture.2003.11.036
  16. Lankford, S.E., Adams, T.E. and Cech, J.J. Jr.: Time of day and water temperature modify the physiological stress response in green sturgeon, Acipensermedirostris. Comp. Biochem. Physiol., 135:291-302, 2003. https://doi.org/10.1016/S1095-6433(03)00075-8
  17. Lee, D.S., Hong, S.H., Lee, H.J., Jun, R.J., Chung, J.K., Kim, K.H. and Jeong, H.D.: Molecular cDNA cloning and analysis of the organization and expression of the$\beta$ gene in the Nile tilapia, Oreochromis niloticus. Comp. Biochem. Physiol., 143:307-314, 2006. https://doi.org/10.1016/j.cbpa.2005.12.014
  18. Miller, N.W., Deuter, A. and Clen, L.W.: Phylogeny of lymphocyte heterogeneity : the cellular requirements for the MLR in channel catfish. Immunol., 59:123-128, 1986.
  19. Morvan, C.L., Troutaud, D. and Deschaux, P.: Differential effects of temperature on specific and nonspecific immune defences in fish. J. Exp. Biol., 201:195-168, 1998.
  20. Nakanishi, T.: Kinetics of transfer of immunity by immune leukocytes and PFC response to HRBC in isogenic ginbuna crucian carp. J. Fish Dis., 30:723-729, 1987.
  21. Pourreau, C.N., Evenberg, D., de Raadt, W.M., van Mechelen, J.A.N. and van Muiswinkel, W.B.: Does Aeromonas salmonicida affect the immune system of carp, Cyprinus carpio L. Vet. Immunol. Immunopathol., 12:331-338, 1986. https://doi.org/10.1016/0165-2427(86)90137-6
  22. Rijkers, G.T., Frederix-Wolters, E.M.H. and van Muinswinkel, W.B.: The immune system of cyprinid fish. Kinetics and temperature dependence of antibody producing cells in carp (Cyprinus carpio). Immunol., 41:91-97, 1980.
  23. Secombes, C.J., White, A., Fletcher, T.C. and Houlihan, D.F.: The development of an ELISPOT assay to quantify total and specific antibody secreting cells in dab Limanda limanda (L). Fish Shellfish Immunol., 1:87-89, 1991. https://doi.org/10.1016/S1050-4648(06)80009-4
  24. Tort, L., Balasch, J.C. and Machenzie, S.: Fish immune system. A crossroads between innate and adaptive responses. Inmunologia, 22:277-286, 2003.
  25. Vainikka, A., Jokinen, E.I., Kortet, R., Sinisalo, T. and Taskinen, J.: Gender- and season-dependent relationships between testosterone, oestradiol and immune functions in wild roach. J. Fish Biol., 64:227-240, 2004. https://doi.org/10.1111/j.1095-8649.2004.00306.x
  26. Waterstrat, P.R., Ainsworth, A.J. and Capley, G. : In vitro responses of channel catfish (Ictalurus punctatus) neutrophils to Edwardsiella ictaluri. Dev. Comp. Immunol., 15:53-63. 1991. https://doi.org/10.1016/0145-305X(91)90047-3
  27. Wilson, M.R.. and Warr, G.W.: Fish immunoglobulins and the genes that encode them. Annu. Rev. Fish Dis., 2:201-221, 1992. https://doi.org/10.1016/0959-8030(92)90064-5