BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Soluble expression, purification and the role of C-terminal glycine residues in scorpion toxin BmK AGP-SYPU2

  • Zhang, Rong (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University) ;
  • Cui, Yong (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University) ;
  • Zhang, Xi (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University) ;
  • Yang, Zhuo (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University) ;
  • Zhao, Yongshan (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University) ;
  • Song, Yong-Bo (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University) ;
  • Wu, Chunfu (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University) ;
  • Zhang, Jinghai (School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University)
  • 투고 : 2010.10.07
  • 심사 : 2010.11.05
  • 발행 : 2010.12.31
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초록

The existence of glycine residues in long-chain scorpion toxins has been well documented. However, their role as analgesics has not been evaluated. To address this issue, we investigated the functional role of glycines in the C-terminal end of Chinese-scorpion toxin from Buthus martensii Karsch (BmK AGP-SYPU2) using site-directed mutagenesis and analgesic activity assays. Recombinant BmK AGP-SYPU2 and its mutants were efficiently expressed in E. coli and purified to homogeneity using immobilized metal ion affinity chromatography (IMAC) and cation exchange chromatography. The mouse-twisting test was used to detect the analgesic activity of BmK AGP-SYPU2 and its mutants. As a result, we identified glycines at the C-terminal end that, when altered, significantly affected analgesic activity. Also, Mut6566 was significantly decreased compared to BmK AGP-SYPU2. These data indicate that the glycines at the C-terminal end are important for the analgesic activity of BmK AGP-SYPU2.

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피인용 문헌

  1. The role of glycine residues at the C-terminal peptide segment in antinociceptive activity: a molecular dynamics simulation vol.19, pp.3, 2013, https://doi.org/10.1007/s00894-012-1666-y
  2. Site-Directed Mutagenesis of BmK AGP-SYPU1: The Role of Two Conserved Tyr (Tyr5 and Tyr42) in Analgesic Activity vol.33, pp.2, 2014, https://doi.org/10.1007/s10930-014-9547-0
  3. The role of Ser54 in the antinociceptive activity of BmK9, a neurotoxin from the scorpion Buthus martensii Karsch vol.58, pp.6-7, 2011, https://doi.org/10.1016/j.toxicon.2011.08.014
  4. Biochemical characterization of ferredoxin-NADP+ reductase interaction with flavodoxin in Pseudomonas putida vol.45, pp.8, 2012, https://doi.org/10.5483/BMBRep.2012.45.8.071