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Effect of Combination of Chitosan Coating and Gamma Irradiation on the Foodborne Pathogen Reduction and Nutritional Properties of Chicken Egg

키토산 코팅 및 감마선 조사에 의한 계란에 오염된 위해 미생물 제어 및 영양학적 특성 평가

  • Kim, Hyun-Joo (Department of Food Science and Technology, Chung-Ang University) ;
  • Yun, Hye-Jeong (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Jung, Samooel (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Jung, Yeon-Kuk (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Ham, Jun-Sang (Division of Quality Control and Utilization, National Institute of Animal Science) ;
  • Jin, Shil (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Jo, Cheorun (Department of Animal Science and Biotechnology, Chungnam National University)
  • 김현주 (중앙대학교 식품공학과) ;
  • 윤혜정 (충남대학교 동물자원생명과학과) ;
  • 정사무엘 (충남대학교 동물자원생명과학과) ;
  • 정연국 (충남대학교 동물자원생명과학과) ;
  • 함준상 (국립축산과학원 축산물이용과) ;
  • 진실 (충남대학교 동물자원생명과학과) ;
  • 조철훈 (충남대학교 동물자원생명과학과)
  • Received : 2010.02.22
  • Accepted : 2010.03.10
  • Published : 2010.03.31

Abstract

The effect of combination of chitosan coating and gamma irradiation on Escherichia coli inactivation and nutritional properties of shell egg was investigated. The E. coli inoculated on shell egg was not detected by 2 kGy of gamma irradiation at day 0 and/or chitosan coating (1%, pH 5.0) after 3 days of storage. There was no E. coli detected thereafter. In the contents of moisture, crude fat, crude protein, ash, retinol, phospholipid, and minerals, no difference was found by treatment combination. However, the contents of cholesterol and carotenoids were reduced by irradiation of 2 kGy (P<0.05). Results suggest that the combination of gamma irradiation (2 kGy) and chitosan coating (1%) can be a good method to improve the safety and prolonged shelf-life of egg because of effective pathogen inactivation without significant adverse changes in nutritional quality.

본 연구는 계란의 위생화 및 저장성 연장에 관한 연구의 일환으로 키토산 코팅 및 감마선 조사 병용처리에 의한 계란에 오염된 위해 미생물 제어 및 영양학적 특성을 평가하였다. 키토산 코팅과 감마선 조사 병용처리한 계란에 E. coli를 접종한 결과 2 kGy 방사선 조사에 의해 검출한계 이하 수준으로 감소하였으며, 키토산 코팅시 3일 후부터 검출되지 않았다. 수분, 조단백질 및 조회분 등 일반 성분은 난백 및 난황 모두에서 차이를 나타내지 않았으며, retinol, 인지질과 미량 원소의 함량도 처리에 따른 차이가 없었다. 난황의 콜레스테롤 및 carotenoid의 함량은 키토산 코팅 유무와 상관없이 방사선 조사에 의해 그 함량이 감소하였다(P<0.05). 따라서 키토산 코팅(1%)과 감마선 조사(2 kGy) 병용처리는 계란의 미생물학적 안전성을 확보할 수 있으며, 영양학적 성분변화에 큰 영향을 끼치지 않아, 향후 계란의 위생화 및 저장성 연장을 위해 산업적으로 적용이 가능할 것이라고 판단된다.

Keywords

References

  1. Ahn DU, Nam KC, Du M, Jo C 2001 Effect of irradiation and packaging conditions after cooking on the formation of cholesterol and lipid oxidation products in meats during storage. Meat Sci 57:413-418. https://doi.org/10.1016/S0309-1740(00)00119-4
  2. AOAC 1984 Official Methods of Analysis of AOAC International, fourteenth ed. Association of Official Analytical Chemists International, Arlington, VA.
  3. Asaoka K 1996 Chito-Chitosanp The Choice Food Supplement for over 10,000 Physicians in Japan. Vantage Press, Inc.
  4. Badr HM 2006 Effect of gamma radiation and cold storage on chemical and organoleptic properties and microbiological status of liquid egg white and yolk. Food Chem 97:285-293. https://doi.org/10.1016/j.foodchem.2005.05.004
  5. Chee KM 2004 Perspective on modifying fatty acid composition and cholesterol content of eggs. Korean J Poult Sci 31: 61-71.
  6. FAO/IAEA/WHO 1999 High-dose irradiation: Wholesomeness of food irradiated with doses above 10 kGy. pp 49-77. In WHO technical report series 890. World Health Organization, Geneva.
  7. Farag RS, Daw Z, Shallan MA, Ebtesam AM 1994 Biochemical and microbial studies on the efficiency of some coating materials for egg preservation. Int J Food Sci Nutr 45:263-273. https://doi.org/10.3109/09637489409166167
  8. Folch J, Less M, Sloane-Stanley GM 1957 A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497-509.
  9. Gast RK, Holt PS, Murase T 2005 Penetration of Salmonella enteritidis and Salmonella heidelberg into egg yolks in an in vitro contamination model. Poult Sci 84:621-625. https://doi.org/10.1093/ps/84.4.621
  10. Hong W, Jeong Y, Ahn Y 2007 Effects of a sodium alginate coating on egg quality during storage. J East Asian Soc Dietary Life 17:822-826.
  11. Imai C, Nanba E 1989 Knowledge of Eggs. Saiwaishobo, Tokyo, Japan.
  12. Kim DH, Ahn HJ, Yook HS, Kim MJ, Sohn CB, Byun MW 2000 Quality properties of gamma irradiated Samjang, seasoned soybean paste during storage. Korean J Food Sci Technol 32:396-401.
  13. Kim DH, Yun HJ, Song HP, Lim BR, Jo C 2008 Isolation of egg-contaminating bacteria and evaluation of bacterial radiation sensitivity. Korean J Food Preserv 15:774-781.
  14. Liu XD, Jang A, Kim DH, Lee BD, Lee M, Jo C 2009 Effect of combination of chitosan coating and irradiation on physicochemical and functional properties of chicken egg during room temperature storage. Radiat Phys Chem 78:585-587. https://doi.org/10.1016/j.radphyschem.2009.03.014
  15. Nishimura K, Nishimura S, Nishi N, Saiki L, Tokura S, Azuma I 1984 Immunological activity of chitin and its derivatives. Vaccine 2:93-99. https://doi.org/10.1016/S0264-410X(98)90039-1
  16. Shin SO, Chi JH, Chen YJ, Yoo JS, Kim HJ, Wang W, Huang Y, Kim IH 2008 Effects of mineral-and vitamin-enhanced supplementation on egg production, egg quality and concentration of calcium and phosphorus in serum of spent laying hens. Korean J Poult Sci 35:21-27. https://doi.org/10.5536/KJPS.2008.35.1.021
  17. Sudarshan NR, Hoover DG, Knorr D 1992 Antibacterial action of chitosan. Food Biotechnol 6:257-272. https://doi.org/10.1080/08905439209549838
  18. Thayer DW 1994 Wholesomeness of irradiated foods. Food Technol 48:58-67.
  19. WHO 1994 Safety and nutritional adequacy of irradiated food. pp 135-143 Geneva: World Health Organization.
  20. Yang SJ, Jung IC, Moon YH 2008 Effects of feeding citrus byproducts on nutritional properties of Korean native chicken eggs. J Korean Soc Food Sci Nutr 37:841-846. https://doi.org/10.3746/jkfn.2008.37.7.841
  21. Yoo JS, Kim JD, Cho JH, Chen YJ, Kim HJ, Min BJ, Kang DK, Kim IH 2006 The effect of natural mineral complex and chitosan supplementation on egg production and characteristics in laying hens. Korean J Poult Sci 33:309-316.
  22. Yoo YM 2003 Changes in quality of eggs with storage temperature and time. Monthly Poultry 3:119-124.

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