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돌단풍 잎 용매추출물의 생리활성

Biological Activities of Solvent Extracts from Leaves of Aceriphyllum rossii

  • 임상현 (강원도농업기술원 농산물이용시험장) ;
  • 김희연 (강원도농업기술원 농산물이용시험장) ;
  • 박민희 (강원도농업기술원 농산물이용시험장) ;
  • 박유화 (강원도농업기술원 농산물이용시험장) ;
  • 함헌주 (강원도농업기술원 농산물이용시험장) ;
  • 이기연 (강원도농업기술원 농산물이용시험장) ;
  • 김경희 (강원도농업기술원 농산물이용시험장) ;
  • 박동식 (농촌진흥청 국립농업과학원 한식세계화연구단 기능성식품과) ;
  • 김성문 (강원대학교 바이오자원환경학과)
  • Lim, Sang-Hyun (Agriproduct Processing Experiment Station Gangwon-do Agicultural Research and Experiment Services) ;
  • Kim, Hee-Yeon (Agriproduct Processing Experiment Station Gangwon-do Agicultural Research and Experiment Services) ;
  • Park, Min-Hee (Agriproduct Processing Experiment Station Gangwon-do Agicultural Research and Experiment Services) ;
  • Park, Yu-Hwa (Agriproduct Processing Experiment Station Gangwon-do Agicultural Research and Experiment Services) ;
  • Ham, Hun-Ju (Agriproduct Processing Experiment Station Gangwon-do Agicultural Research and Experiment Services) ;
  • Lee, Ki-Yun (Agriproduct Processing Experiment Station Gangwon-do Agicultural Research and Experiment Services) ;
  • Kim, Kyung-Hee (Agriproduct Processing Experiment Station Gangwon-do Agicultural Research and Experiment Services) ;
  • Park, Dong-Sik (National Academy of Agricultural Science) ;
  • Kim, Song-Mun (Dept. of Biological Environment, Kangwon National University)
  • 투고 : 2010.11.05
  • 심사 : 2010.12.06
  • 발행 : 2010.12.31

초록

돌단풍 잎의 항산화, 항염, 항당뇨, 항비만 활성을 탐색하고자 강원도 양구에서 자생하고 있는 돌단풍의 잎을 채집하여 에탄올과 증류수로 각각 추출하였다. 항산화 활성은 DPPH 라디칼 소거반응, 총 폴리페놀 함량 및 총 플라보노이드 함량을 측정하여 검정하였다. 돌단풍 잎의 에탄올과 물추출물의 DPPH 라디칼 소거능 $IC_{50}$값은 각각 549.86과 $62.14{\mu}g$/mL이었고, 총 폴리페놀 함량은 에탄올 추출물이 307.63 mg/g으로 물 추출물의 총 폴리페놀 함량인 86.96 mg/g보다 높게 나타났으나 총 플라보노이드 함량은 에탄올과 물 추출물 각각 118.98과 110.16 mg/g으로 비슷한 함량을 나타냈다. 항염 활성은 RAW264.7 세포에 LPS와 돌단풍 잎추출물을 동시에 10 mg/mL 처리하여 NO 생성량을 측정하였으나 효과는 없었다. 항당뇨 활성은 $\alpha$-glucosidase와 $\alpha$-amylase 저해활성을 측정하였으며, 돌단풍 에탄올과 물 추출물의 $\alpha$-glucosidase $IC_{50}$값은 각각 5.62와 $425.63{\mu}g$/mL이었고, $\alpha$-amylase $IC_{50}$값은 각각 4,623.87과 $10,000{\mu}g$/mL 이상으로 돌단풍 에탄올 추출물의 항당뇨 활성은 매우 뛰어났다. 항비만 활성 탐색은 pancreatic lipase의 활성저해를 측정하였으며, 그 결과 돌단풍 잎의 에탄올과 물 추출물의 $IC_{50}$ 값은 모두 $10,000{\mu}g$/mL 이상으로 항비만 활성은 없는 것으로 나타났다. 이러한 결과 돌단풍 잎 추출물의 항산화, 항당뇨의 기능성활용 가공식품 및 건강기능식품으로의 개발이 기대된다.

In this study, the bioactivities of ethanol (EEAR) and water extract (WEAR) from the leaf of Aceriphyllum rossii were investigated. In the anti-oxidative activity, IC50 of DPPH radical scavenging activity was respectively 549.86 and $62.14{\mu}g$/mL by EEAR and WEAR. Anti-inflammatory activity of EEAR and WEAR has been evaluated on inhibition of lipopolysaccharide (LPS)-induced nitric oxide (NO) release by the macrophage RAW 264.7 cells. EEAR and WEAR inhibited inflammatory by 5.58 and 16.85% in 10 mg/mL, respectively. In the anti-diabetic activity, $IC_{50}$ of $\alpha$-glucosidase inhibitory activity was 5.62 and $425.63{\mu}g$/mL by EEAR and WEAR. $IC_{50}$ of $\alpha$-amylase inhibitory activity of EEAR and WEAR was 4,623.87 and over $10,000{\mu}g$/mL, respectively. In the anti-obesity, all lipase inhibitory activity ($IC_{50}$) of EEAR and WEAR was up $10,000{\mu}g$/mL. Finally, EEAR and WEAR exhibited anti-oxidative and anti-diabetic activity. It suggests that Aceriphyllum rossii could be potentially used as a resource of bioactive materials for health functional foods.

키워드

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