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Biological Probiotic Properties of Lactobacillus rhamonosus GG-4 Isolated from Infant Feces

유아분변으로부터 분리한 Lactobacillus rhamonosus GG-4의 생균제적 특성

  • Kang, Jin-Hae (Department of Pharmaceutical Engineering, JinJu National University) ;
  • Kim, Dae-Hwan (Department of Pharmaceutical Engineering, JinJu National University) ;
  • Lee, Sang-Won (Department of Pharmaceutical Engineering, JinJu National University) ;
  • Kim, Hong-Chul (Department of Pharmaceutical Engineering, JinJu National University) ;
  • Cho, Young-Un (Department of Pharmaceutical Engineering, JinJu National University) ;
  • Gal, Sang-Wan (Department of Pharmaceutical Engineering, JinJu National University)
  • 강진혜 (진주산업대학교 제약공학과) ;
  • 김대환 (진주산업대학교 제약공학과) ;
  • 이상원 (진주산업대학교 제약공학과) ;
  • 김홍출 (진주산업대학교 제약공학과) ;
  • 조용운 (진주산업대학교 제약공학과) ;
  • 갈상완 (진주산업대학교 제약공학과)
  • Received : 2010.11.05
  • Accepted : 2010.12.15
  • Published : 2010.12.30

Abstract

To develop probiotics, a kind of Lactobacillus sp. was isolated from infant feces. The bacterium was identified as Lactobacillus rhamnosus through 16S rDNA sequence analysis. The strain was a facultative anaerobe which grew better in aerobic conditions. The bacterium lowered the pH of the culture solution down to 2.4 during 48 hr in the MRS medium. The strain inhibited the growth of 6 pathogens - S. aureus, L. monocytogens, S. typhimurium, E. coli O-157, V. parahaemolyticus and P. aeruginosa. When the Lactobacillus were fed to chickens, along with commercial feed, for one month, amounts of $H_2S$ and $NH_3$ in the feces of the chicken decreased to 50% and 70%, respectively, compared to those of control group chickens. Amounts of other bad smells such as $(CH_3)SH$, $(CH_3)_2S$ and $(CH_3)_2S_2$ were not much different in the Lactobacillus-fed chickens compared to the control group. On the other hand, egg weights of the chickens fed Lactobacillus were higher by about $5{\pm}1\;g$ than those in the control group.

생균제 개발을 위하여 한 종류의 유산균을 유아 분변으로부터 분리하였다. 이 세균을 16s rDNA 염기서열 분석을 통하여 확인해 본 결과 Lactobacillus rhamnosus로 동정되었다. 이 유산균은 호기성 조건에서도 잘 자라는 통성 혐기성 세균이었다. 이 유산균은 MRS 배지에 48 시간 액체 배양하였을 때 배양액의 pH를 2.4까지 낮추는 것을 확인 하였다. 이 유산균은 6 종의 병원성 세균, L. monocytogenes, S. aureus, P. aeruginosa, V. parahaemolyticus, S. typhimurium, and E. coli O157의 생육을 저해하는 것을 확인하였다. 이 유산균을 시판 중인 닭 사료에 섞어 먹인 후 분변의 악취를 조사하였을 때, 분변의 악취 중 약 80 %를 차지하는 황화수소는 대조구 보다 50 %가 감소하였고, 암모니아는 상대적으로 함유된 양은 적었지만 대조구 보다 70 %가 감소하였음을 확인할 수 있었다. 유산균 급이구와 대조구의 외형은 변화가 없었으며, 달걀 무게에 있어서 먹인 닭의 달걀이 평균 5 g 정도 무거워진 것을 확인 할 수 있었다.

Keywords

References

  1. Chan, R. C. Y., G. Reid, R. T. Irvin, A. W. Bruce, and J. W. Costerton. 1985. Competitive exclusion of uropathogens from human uroepitherial cells by Lactobacillus whole cells and cell wall fragment. Infect. Immun. 47, 84-89.
  2. Fernandes, C. F., K. M. Shahani, and M. A. Amer. 1987. Therapeutic role of dietary Lactobacilli and lactobacillic fermented dairy products. FEMS Microbiol. Rev. 46, 343-356. https://doi.org/10.1111/j.1574-6968.1987.tb02471.x
  3. Gilliland, S. E. and M. L. Speck. 1977. Antagonistic action of Lactoba-cillus acidophilus toward intestinal and foodborne pathogens in associative cultures. J. Food Prot. 40, 820-823.
  4. Gilliland, S. E. and D. K. Walker. 1990. Factors to consider when selecting a culture of Lactobacillus acidophilus as a dietary adjunct to produce a hypocholesterolemic effect in humans. J. Dairy Sci. 73, 905-911. https://doi.org/10.3168/jds.S0022-0302(90)78747-4
  5. Isolauri, E., J. Joensuu, H. Suomalainen, M. Luomala, and T. Vesikari. 1995. Improved immunogenicity of oral Dx RRV reassortant rotavirus vaccine by Lactobacillus casei GG. Vaccine 13, 310-312. https://doi.org/10.1016/0264-410X(95)93319-5
  6. Jang, Y. D., H. K. Oh, L. G. Piao, H. B. Choi, J. H. Yum, and Y. Y. Kim. Evaluation of probiotics as an alternative to antibiotic on growth performance, nutrient digestibility, occurrence of diarrhea and immune response in weaning pigs. 2009. J. Anim. Sci. Technol. (Kor.) 51, 25-32. https://doi.org/10.5187/JAST.2009.51.1.025
  7. Kitazawa, H., K. Matsumura, T. Itoh, and T. Yamaguch. 1992. Interferon inductionn in murine peritoneal macrophage by stiulation with Lactobacillus acidophilus. Microbiol. Immunol. 36, 311-315. https://doi.org/10.1111/j.1348-0421.1992.tb01668.x
  8. Klaenhammer, T. R. 1993. Gemetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev. 12, 39-85. https://doi.org/10.1111/j.1574-6976.1993.tb00012.x
  9. Lee, E. Y. 2008. Problems and verification system of probiotics as livestock-environment improving agent produced and circulated. Korean J. Microbiol. Biotechnol. 36, 87-95.
  10. Lee, S. B. and S. H. Choi. 2006. Isolation and identification of probiotic Lactobacillus isolates for Calf Meal Supplements. Korean J. Food. Sci. Ani. Resour. 26, 106-112.
  11. Lee, S. H., E. H. Yang, H. S. Kwon, J. H. Kang, and B. H. Kang. 2008. Potential probiotic properties of Lactobacillus johnsonii IDCC 9203 isolated from infant feces. Korean J. Microbiol. Biotechnol. 36, 121-127.
  12. Lee, S. H. and M. J. No. 1997. Viability in artificial gastric and bile juice and antimicrobial activity of some lactic acid bacteria isolated from Kimchi. Korean J. Microbiol. Biotechnol. 25, 617-622.