Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Induction of Apoptosis by Ethanol Extracts of Fermented Agabeans in AGS Human Gastric Carcinoma Cells

AGS 인체위암세포에서 발효된 아가콩 추출물에 의한 apoptosis 유도

  • Kim, Sung-Ryeal (Department of Biotechnology, Dong-A University) ;
  • Lee, Hye-Hyeon (Department of Biotechnology, Dong-A University) ;
  • Kim, Min-Jeong (Department of Biotechnology, Dong-A University) ;
  • Seo, Min-Jeong (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Hong, Su-Hyun (Department of Biochemistry, College of Oriental Medicine and Research Institute of Oriental Medicine, Department of Biomaterial Control, Graduate School and Blue-Bio Industry Regional Innovation Center, Dongeui University) ;
  • Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine and Research Institute of Oriental Medicine, Department of Biomaterial Control, Graduate School and Blue-Bio Industry Regional Innovation Center, Dongeui University) ;
  • Kang, Byoung-Won (Department of Biotechnology, Dong-A University) ;
  • Park, Jeong-Uck (Department of Biotechnology, Dong-A University) ;
  • Joo, Woo-Hong (Department of Biology, Changwon National University) ;
  • Rhu, Eun-Ju (Department of Cosmetology, Han Seo University) ;
  • Jeong, Yong-Kee (Department of Biotechnology, Dong-A University)
  • 김성열 (동아대학교 생명공학과) ;
  • 이혜현 (동아대학교 생명공학과) ;
  • 김민정 (동아대학교 생명공학과) ;
  • 서민정 (동아대학교 Medi-Farm 산업화 연구사업단) ;
  • 홍수현 (동의대학교 한의과대학 생화학교실 및 한의학연구소, 대학원 바이오물질제어학과 및 블루바이오 소재개발센터) ;
  • 최영현 (동의대학교 한의과대학 생화학교실 및 한의학연구소, 대학원 바이오물질제어학과 및 블루바이오 소재개발센터) ;
  • 강병원 (동아대학교 생명공학과) ;
  • 박정욱 (동아대학교 생명공학과) ;
  • 주우홍 (창원대학교 생물학과) ;
  • 류은주 (한서대학교 피부미용학과) ;
  • 정영기 (동아대학교 생명공학과)
  • Received : 2010.11.04
  • Accepted : 2010.12.13
  • Published : 2010.12.30
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Abstract

Extracts of soybeans fermented by Bacillus subtilis have a wide variety of functions, such as enhancing the body's immune function, fibrinolysis activity, anti-inflammation, anti-cancer, estrogen function and anti-infection effects. Recently, it was reported that the extracts of fermented beans exhibit strong anti-inflammatory and anti-cancer properties by suppressing the transcription of pro-inflammatory cytokine genes and induction of apoptosis, respectively. However, the mechanisms of their cytotoxicity in human gastric cancer cells are poorly understood. In the present study, we investigated the effects of ethyl alcohol extracts from fermented soybean (FS) and yellow agabean (FYA) on cell growth and apoptosis in AGS human gastric cancer cells. A treatment of FS and FYA inhibited the growth of AGS cells in a concentration-dependent manner by inducing apoptosis. FS- and FYA-induced apoptosis were associated with down-regulation of XIAP and cIAP-2, and up-regulation of pro-apoptotic Bax expression. Moreover, a treatment of FS and FYA not only triggered an increase in the levels of death receptor (DR)4, DR5, Fas and FasL, but also induced the activation of casepase-3, -8 and -9. These findings illustrate that FS and FYA may have a therapeutic potential in human gastric AGS cells and as a functional food.

본 연구에서는 대두(FS)와 아가콩의 발효추출물(FYA)의 항암활성 기전을 확인하기 위해 AGS 인체위암세포의 증식에 미치는 영향을 조사하였다. AGS 세포에서 FS 및 FYA 처리로 인하여 암세포의 증식이 처리 농도 의존적으로 강하게 억제하였고, apoptosis 유발을 의미하는 세포의 전반적인 형태 및 핵의 변형 또한 동반하였다. 또한 세포주기 분석을 통하여 이 현상이 apoptosis 유도에 의한 것임을 확인하였다. AGS 세포에 처리된 FS 및 FYA는 pro-apoptotic factor인 Bax의 발현 증가를 통한 intrinsic pathway나, death receptor 관련 유전자의 발현 증가를 통한 extrinsic pathway를 활성화시키며, 더 나아가서 IAP family인자의 발현 억제 및 caspases의 활성 증가를 일으켜 apoptosis를 유발시키는 것을 유추할 수 있었는데, 이러한 효과들은 FS보다 FYA에서 더욱더 탁월하였다. 이는 향후 아가콩 발효추출물이 항암치료를 위한 적용 가능성이 매우 우수함을 제시하여 주는 결과이다.

Keywords

References

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