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Genetic Quality Control of the Rat Strains at the National Bio Resource Project-Rat

  • Kuramoto, Takashi (Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University) ;
  • Nakanishi, Satoshi (Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University) ;
  • Yamasaki, Ken-ichi (Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University) ;
  • Kumafuji, Kenta (Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University) ;
  • Sakakibara, Yuichi (Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University) ;
  • Neoda, Yuki (Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University) ;
  • Takizawa, Akiko (Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University) ;
  • Kaneko, Takehito (Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University) ;
  • Otsuki, Mito (Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University) ;
  • Hashimoto, Ryoko (Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University) ;
  • Voigt, Birger (Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University) ;
  • Mashimo, Tomoji (Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University) ;
  • Serikawa, Tadao (Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University)
  • Received : 2010.11.12
  • Accepted : 2010.11.19
  • Published : 2010.12.31

Abstract

The National Bio Resource Project-Rat (NBRP-Rat) comprises the largest bank of laboratory rat (Rattus norvegicus) strains in the world. Its main focus is to develop infrastructure that will facilitate the systematic collection, preservation, and provision of rat strains. To breed effectively more than 180 rat strains in living stock, we establish the genetic control system in which a systematic set of genetic diagnoses and genetic monitoring are included. Genetic monitoring is performed by using 20 polymorphic markers. Monitoring is carried out when a living animal stock is re-established by using cryopreserved embryos or sperm or when a rat strain is first introduced to the NBRP-Rat by a depositor. Additional monitoring is then carried out on each strain every two years. Genetic diagnosis is performed largely by employing the Amp-FTA method. Protocols which detail how to perform a genetic diagnosis of 11 transgenes and 24 mutations have been made. Among the mutations, nine can be detected by simple gel electrophoresis of the PCR products, 11 by restriction enzyme treatment of the PCR products, and four by direct PCR product sequencing. Using this genetic control system, the NBRP-Rat can guarantee the genetic quality of its rat strains.

Keywords

References

  1. Aitman, T.J., Critser, J.K., Cuppen, E., Dominiczak, A., Fernandez-Suarez, X.M., Flint, J., Gauguier, D., Geurts, A.M., Gould, M., Harris, P.C., et al. (2008). Progress and prospects in rat genetics: a community view. Nat Genet 40, 516-522. https://doi.org/10.1038/ng.147
  2. Serikawa, T., Mashimo, T., Takizawa, A., Okajima, R., Maedomari, N., Kumafuji, K., Tagami, F., Neoda, Y., Otsuki, M., Nakanishi, S., et al. (2009). National BioResource Project-Rat and related activities. Exp Anim 58, 333-341. https://doi.org/10.1538/expanim.58.333
  3. Bryda, E.C., and Riley, L.K. (2008). Multiplex microsatellite marker panels for genetic monitoring of common rat strains. J Am Assoc Lab Anim Sci 47, 37-41.
  4. Nitzki, F., Kruger, A., Reifenberg, K., et al. (2007). Identification of a genetic contamination in a commercial mouse strain using two panels of polymorphic markers. Lab Anim 41, 218-228. https://doi.org/10.1258/002367707780378104
  5. Nakanishi, S., Kuramoto, T., Serikawa, T. (2009). Simple genotyping method using Ampdirect plus and FTA technologies: application to the identification of trangenic animals and their rutine genetic monitoring. Lab Anim Res 25, 75-78.
  6. Yabutani, M., Agata, N., and Ohta, M. (2009). A new rapid and sensitive detection method for cereulide-producing Bacillus cereus using a cycleave real-time PCR. Lett Appl Microbiol 48, 698-704.
  7. Yoshimi, K., Tanaka, T., Takizawa, A., Kato, M., Hirabayashi, M., Mashimo, T., Serikawa, T., and Kuramoto, T. (2009). Enhanced colitis-associated colon carcinogenesis in a novel Apc mutant rat. Cancer Sci 100, 2022-2027. https://doi.org/10.1111/j.1349-7006.2009.01287.x
  8. Tong, C., Li, P., Wu, N.L., Yan, Y., and Ying, Q.L. (2010). Production of p53 gene knockout rats by homologous recombination in embryonic stem cells. Nature 467, 211-213. https://doi.org/10.1038/nature09368
  9. Voigt, B., and Serikawa, T. (2009). Pluripotent stem cells and other technologies will eventually open the door for straightforward gene targeting in the rat. Dis Model Mech 2, 341-343. https://doi.org/10.1242/dmm.002824
  10. Kuramoto, T., Yokoe, M., Yagasaki, K., Kawaguchi, T., Kumafuji, K., and Serikawa, T. (2010). Genetic analyses of fancy rat-derived mutations. Exp Anim 59, 147-155. https://doi.org/10.1538/expanim.59.147
  11. Zhang, Q., Zhao, B., Li, W., Oiso, N., Novak, E.K., Rusiniak, M.E., Gautam, R., Chintala, S., O'Brien, E.P., Zhang, Y., et al. (2003). Ru2 and Ru encode mouse orthologs of the genes mutated in human Hermansky-Pudlak syndrome types 5 and 6. Nat Genet 33, 145-153. https://doi.org/10.1038/ng1087
  12. Nanashima, N., Akita, M., Yamada, T., et al. (2008). The hairless phenotype of the Hirosaki hairless rat is due to the deletion of an 80-kb genomic DNA containing five basic keratin genes. J Biol Chem 283, 16868-16875. https://doi.org/10.1074/jbc.M802539200
  13. Hanada, K., Chiyoya, S., Suzuki, K., Hashimoto, I., and Hatayama, I. (1988). Study of the skin of a new hairless rat mutant. J Dermatol 15, 257-262. https://doi.org/10.1111/j.1346-8138.1988.tb03687.x
  14. Keino, H., Sato, H., Semba, R., Aono, S., Aoki, E., and Kashiwamata, S. (1985). Mode of prevention by phototherapy of cerebellar hypoplasia in a new Sprague-Dawley strain of jaundiced Gunn rats. Pediatr Neurosci 12, 145-150. https://doi.org/10.1159/000120237
  15. Kuramoto, T., Kitada, K., Inui, T., Sasaki, Y., Ito, K., Hase, T., Kawagachi, S., Ogawa, Y., Nakao, K., Barsh, G.S., et al. (2001). Attractin/mahogany/zitter plays a critical role in myelination of the central nervous system. Proc Natl Acad Sci U S A 98, 559-564. https://doi.org/10.1073/pnas.98.2.559
  16. Taylor, B.A., and Phillips, S.J. (1996). Detection of obesity QTLs on mouse chromosomes 1 and 7 by selective DNA pooling. Genomics 34, 389-398. https://doi.org/10.1006/geno.1996.0302

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