Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Biodegradation of Fungicide Tolclofos-methyl by Sphingomonas sp. 224

Sphingomonas sp. 224 균주에 의한 살균제 tolclofos-methyl의 분해

  • Kwak, Yun-Young (School of Applied Biosciences, Kyungpook National University) ;
  • Shin, Kab-Sik (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, Sang-Man (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Jang-Eok (School of Applied Biosciences, Kyungpook National University) ;
  • Rhee, In-Koo (School of Applied Biosciences, Kyungpook National University) ;
  • Shin, Jae-Ho (School of Applied Biosciences, Kyungpook National University)
  • 곽윤영 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 신갑식 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 이상만 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 김장억 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 이인구 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 신재호 (경북대학교 농업생명과학대학 응용생명과학부)
  • Received : 2010.12.10
  • Accepted : 2010.12.20
  • Published : 2010.12.30
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Abstract

In order to decrease level of an organophosphorus fungicide, tolclofos-methyl, from in situ ginseng cultivating soil, we isolated a tolclofos-methyl degrading bacteria from ginseng cultivating soil samples. The bacterial strain removed tolclofos-methyl around 95% after 3 days incubation with complete liquid media. The strain was identified as Sphingomonas sp. by 16S rDNA sequence comparison, and designated as Sphingomonas sp. 224. Through the GC-MS analysis, Sphingomonas sp. 224 was proposed to have an initiative degradation pathway generating the metabolite such as 2,6-dichloro-4-methyl phenol compound from tolclofos-methyl. In addition, Sphingomonas sp. 224 was confirmed representing the effective degrading capability to tolclofosmethyl in situ soil.

미생물을 이용한 인삼 재배지 내 잔류 tolclofos-methyl의 효과적 분해를 목적으로, tolclofos-methyl에 대한 분해능을 보이는 미생물을 선발하였다. 선발된 미생물은 16S rDNA 염기서열분석을 통하여 Sphingomonas 속으로 동정되었다. 선발 미생물 Sphingomonas sp. 224는 1/10 농도의 LB 배지에 함유된 20 mg/L 농도의 tolclofos-methyl을 배양 72시간 이내에 95% 이상 분해하는 것으로 확인되었다. 또한 이 미생물이 tolclofos-methyl을 분해하여 얻어지는 산물로 2,6-dichloro-4-methyl phenol이 확인됨에 따라 미생물이 생산하는 가수분해 효소에 의한 분해 경로를 가지는 것으로 추정되었다. Tolclofos-methyl 분해 미생물 Sphingomonas sp. 224를 인삼경작지 토양에 처리하여 이들 토양에 잔류되어 있는 tolclofos-methyl에 대한 분해능을 확인 한 결과, 20 mg/Kg 농도의 토양 잔류 tolclofos-methyl에 대하여 14일 이내에 약 50%의 분해력을 보이는 것으로 확인되었다. 이것은 단일 미생물을 이용한 배지 및 토양 내 tolclofosmethyl의 생분해 효과를 처음으로 확인한 연구 결과이다.

Keywords

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