Enzymatic hydrolysis and micro-structure of ozone treated wood meal

오존 처리에 의한 목재 세포벽의 미세구조변화와 효소가수분해

  • Kim, Kang-Jae (Dept. of Wood Science and Technology, College of Agriculture and Life Science, Kyungpook National University) ;
  • Eom, Tae-Jin (Dept. of Wood Science and Technology, College of Agriculture and Life Science, Kyungpook National University)
  • 김강재 (경북대학교 농업생명과학대학 임산공학과) ;
  • 엄태진 (경북대학교 농업생명과학대학 임산공학과)
  • Received : 2010.09.06
  • Accepted : 2010.09.24
  • Published : 2010.09.30

Abstract

Pine (Pinus densiflora) and aspen (Populus euramericana) wood meals were treated with ozone at various time schedule in acidic condition. The lignin contents and surface area of the ozone treated wood meals were determined and the enzymatic hydrolysis rate of ozonated wood meals was evaluated. The feasibility of enzymatic hydrolysis of the ozone treated wood meal was obviously influenced with the degree of delignification. After ozone treatment of wood meal for 10min, total pore volume were slightly increased in the surface of wood meal. When wood meals were treated with ozone longer than 10min, few change in the pore volume was observed. However, the area of over $50{\AA}$ of pore size is increased with ozonation time. As a conclusion, the rate of enzymatic hydrolysis of wood is more effective with the pore size distribution than the total pore volume.

Keywords

References

  1. M.P. Coughlan, Enzymic hydrolysis of cellulose: an overview, Bioresource Technology, 39(2), 107-115 (1991).
  2. R.S. Bes, G. Gas, J. Molinier, P. Vidal, J. Mathieu, J.C. Mora, Enhancement of poplar cellulose susceptibility to cellulase enzyme hydrolysis by ozonation, Science & Engineering, 11(2), 217-226(1989).
  3. A. M. Nadezhda, A.A. Stanislav, G.B. Natal'ya, V.L. Valery, Delignification of softwood by ozonation, Pure Appl. Chem., 81(11), 2081-2091(2009). https://doi.org/10.1351/PAC-CON-08-10-11
  4. Noriko Hayashi, Kazumasa Shimizu, Shuji Hosoya, Pretreatment of ozone for increasing the enzymatic susceptibility of autohydrolyzed softwoods, Mokuzai Gakkaishi, 35(6), 521-529 (1989).
  5. H. Kaneko, S. Hosoya, J. Nakano, Delignification of lignin with ozone(in Japanese), Mokuzai Gakkaishi, 26, 752-758(1980).
  6. Rarid Talebnia, Dimitar Karakashev, Irini Angelidaki, Production of bioethanol from wheat straw: An overview on pretreatment hydrolysis and fermentation, Bioresource Technology, 101, 4744- 4753(2010). https://doi.org/10.1016/j.biortech.2009.11.080
  7. M.J. Taherzadeh, K. Karimi, Acid- based hydrolysis processes for ethanol from lignocellulosic materials: A review, BioResources, 2, 472-499 (2007).
  8. P. Buffiere, D. Liosel, N. Bernet, J.P. Delgenes, Towards new indicators for the prediction of solid waste anaerobic digestion properties, Water Sci. Technol., 53, 233-241(2006). https://doi.org/10.2166/wst.2006.254
  9. Mohammad J. Taherzadeh, Keikhosro Karimi, Pretreatment of lignocellulosic wastes to improve ethanol and biogas production: A review, Int. J. Molecular Sciences, 9, 1621-1651(2008). https://doi.org/10.3390/ijms9091621
  10. L.T. Fan, Y.H. Lee, D.R. Beardmore, The influence of major structural features of cellulose on rate of enzymic hydrolysis, Biotechnol. & Bioeng., 23(2), 419-24(1981).(6) https://doi.org/10.1002/bit.260230215
  11. Hans E. Grethlein, The effect of pore size distribution on the rate of enzymatic hydrolysis of cellulosic substrates, Biotechnology February(1985).
  12. David N. Thompson, Hsin-Chih Chen, Hans E. Grethlein, Comparison of pretreatment methods on the basis of available surface area, Bioresource Technology, 39, 155-163(1992). https://doi.org/10.1016/0960-8524(92)90135-K
  13. C.A. Mooney, S.D. Mansfield, M.G. Touhy, J.N. Saddler, The effect of initial pore volume and lignin content on the enzymatic hydrolysis of softwoods, Bioresourse Technology, 64(2), 113-119(1998). https://doi.org/10.1016/S0960-8524(97)00181-8
  14. D.S. Burns, H. Ooshima, A.O. Converse, Surface area of pretreated lignocellulosics as a function of the extent of enzymatic hydrolysis, Applied Biochem. & Biotechnol., 20, 79-94(1989). https://doi.org/10.1007/BF02936474
  15. A.O. Converse, H. Ooshima, D.S. Burns, Kinetics of enzymatic hydrolysis of lignocellulosic materials based on surface area of cellulose accessible to enzyme and enzyme adsorption on lignin and cellulose, Applied Biochem. & Biotechnol., 24, 67-73(1990). https://doi.org/10.1007/BF02920234
  16. A.P. Sinitsyn, A.V. Gsakov, E.Yu. Vlasenko, Effect of structural and physico-chemical features of cellulosic substrates on the efficiency of enzymic hydrolysis, Applied Biochem. & Biotechnol., 30(1), 43-59(1991). https://doi.org/10.1007/BF02922023
  17. Sannigrahi Poulomi, Miller Stephen J., Ragauskas Arthur J., Effects of organosolv pretreatment and enzymatic hydrolysis on cellulose structure and crystallinity in Loblolly pine, Carbohydrate research, 345 (7), 965-70(2010).(3) https://doi.org/10.1016/j.carres.2010.02.010
  18. L.E. Peters, L.P. Walker, D.B. Wilson, D. C. Irwin, The impact of initial particle size on the fragmentation of cellulose by cellulases of Thermomonospora fusca, Bioresource Technology, 35(3), 313-319 (1991).(12) https://doi.org/10.1016/0960-8524(91)90130-C
  19. C.A. Mooney, S.D. Mansfield, R.P. Beatson, J.N. Saddler, The effect of fiber characteristics on hydrolysis and cellulase accessibility to softwood substrates, Enzyme and Microbial Technology, 25(8-9), 644-650(1999). https://doi.org/10.1016/S0141-0229(99)00098-8