KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Melanin Biosynthesis Inhibitory Effect of New Compound (Jeju-Erythrane) Isolated from Bark of Lindera erythrocarpa Makino

비목나무 (Lindera erythrocarpa Makino) 껍질에서 분리한 신규화합물 (Jeju-Erythrane)의 멜라닌 생합성 억제 효과

  • Kang, Min-Chul (Jeju Bio-Industry Development Center, Jeju Technopark) ;
  • Ko, Ryeo-Kyeong (Jeju Bio-Industry Development Center, Jeju Technopark) ;
  • Kim, Su-Gyeong (Jeju Bio-Industry Development Center, Jeju Technopark) ;
  • Choi, Ho-Min (Jeju Bio-Industry Development Center, Jeju Technopark) ;
  • Jin, Yeong-Jun (Jeju Bio-Industry Development Center, Jeju Technopark) ;
  • Han, Jong-Heon (Jeju Bio-Industry Development Center, Jeju Technopark) ;
  • Kim, Bong-Seok (Jeju Bio-Industry Development Center, Jeju Technopark) ;
  • Lee, Nam-Ho (Department of Chemistry, College of Natural Sciences, Cheju National University) ;
  • Kim, Gi-Ok (Jeju Bio-Industry Development Center, Jeju Technopark)
  • 강민철 ((재)제주테크노파크 생물산업진흥센터) ;
  • 고려경 ((재)제주테크노파크 생물산업진흥센터) ;
  • 김수경 ((재)제주테크노파크 생물산업진흥센터) ;
  • 최호민 ((재)제주테크노파크 생물산업진흥센터) ;
  • 진영준 ((재)제주테크노파크 생물산업진흥센터) ;
  • 한종헌 ((재)제주테크노파크 생물산업진흥센터) ;
  • 김봉석 ((재)제주테크노파크 생물산업진흥센터) ;
  • 이남호 (제주대학교 자연과학대학 화학과) ;
  • 김기옥 ((재)제주테크노파크 생물산업진흥센터)
  • Received : 2010.06.30
  • Accepted : 2010.08.14
  • Published : 2010.08.31
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Abstract

In this study, a new compound, 1-(2-hydroxy-3,4,5,6-tetramethoxyphenyl)-1-methoxy-3-phenylpropane; (Jeju-Erythrane) was isolated and identified from the bark of Lindera erythrocarpa Makino. Also, we investigated the effects of Jeju-Erythrane on alpha melanocyte-stimulating hormone (MSH)-induced melanogenesis in mouse B16F10 melanoma cells. The new compound dose dependently inhibited the tyrosinase activity and melanin synthesis in B16F10 cells. The new compound showed inhibitory effect on the Tyrosinase and TRP-1 gene transcription but not on the TRP-2 gene. These results suggest that the new compound of L. erythrocarpa could be used as a functional biomaterial in developing skin whitening agent.

본 연구는 제주도에서 자생하는 비목나무 (Lindera erythrocarpa) 껍질에서 분리 동정한 신규화합물 (Jeju-Erythrane)의 항산화 활성 및 B16F10 mouse melanoma 세포에서의 tyrosinase와 멜라닌 생합성의 억제효과를 확인하여, 유용자원으로서 미백 및 기능성 화장료로의 산업적 활용 가능성이 있는지 알아보고자 시행하였다. 실험 결과, 신규화합물인 Jeju-Erythrane의 항산화 활성은 확인되지 않았고, B16F10 세포에서 tyrosinase와 멜라닌 합성을 농도 의존적으로 억제하는 것으로 밝혀졌으며, tyrosinase와 TRP-1 유전자의 단백질 및 mRNA 발현양도 농도 의존적으로 감소함을 보였다. 하지만, TRP-2 단백질과 mRNA는 농도에 관계없이 일정하게 발현하는 것을 알 수 있었다. 따라서, 본 연구 결과는 비목나무 (Lindera erythrocarpa) 껍질에서 분리 동정한 신규화합물 (Jeju-Erythrane)이 미백 관련 기능성 소재로서의 활용가치가 있음을 보여준다.

Keywords

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