Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Proteomic Analysis of Toxoplasma gondii KI-1 Tachyzoites

  • Choi, Si-Hwan (Department of Ophthalmology, Chungnam National University School of Medicine) ;
  • Kim, Tae-Yun (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine) ;
  • Park, Sung-Goo (Medical Proteomics Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Cha, Guang-Ho (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine) ;
  • Shin, Dae-Whan (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine) ;
  • Lee, Young-Ha (Department of Infection Biology and Research Institute for Medical Sciences, Chungnam National University School of Medicine)
  • Received : 2010.05.28
  • Accepted : 2010.08.11
  • Published : 2010.09.30
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Abstract

We studied on the proteomic characteristics of Toxoplasma gondii KI-1 tachyzoites which were originally isolated from a Korean patient, and compared with those of the well-known virulent RH strain using 2-dimensional electrophoresis (2-DE), mass spectrometry, and quantitative real-time PCR. Two-dimensional separation of the total proteins isolated from KI-1 tachyzoites revealed up to 150 spots, of which 121 were consistent with those of RH tachyzoites. Of the remaining 29 spots, 14 showed greater than 5-fold difference in density between the KI-1 and RH tachyzoites at a pH of 5.0-8.0. Among the 14 spots, 5 from the KI-1 isolate and 7 from the RH strain were identified using MALDI-TOF mass spectrometry and database searches. The spots from the KI-1 tachyzoties were dense granule proteins (GRA 2,3,6, and 7), hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGRPTase), and uracil phosphoribosyltransferase (UPRTase). The spots from the RH strain were surface antigen 1 (SAG 1), L-lactate dehydrogenase (LDH), actin, chorismate synthase, peroximal catalase, hexokinase, bifunctional dihydrofolate reductase-thymidylate synthase (DHTR-TS), and nucleosidetriphosphatases (NTPases). Quantitative real-time PCR supported our mass spectrometric results by showing the elevated expression of the genes encoding GRA 2,3, and 6 and UPRTase in the KI-1 tachyzoites and those encoding GRA 7, SAG 1, NTPase, and chorismate synthase in the RH tachyzoites. These observations demonstrate that the protein compositions of KI-1 and RH tachyzoites are similar but differential protein expression is involved in virulence.

Keywords

References

  1. Dodds EM. Toxoplasmosis. Curr Opin Ophthalmol 2006; 17: 557-561. https://doi.org/10.1097/ICU.0b013e32801094ca
  2. Howe DK, Sibley LD. Toxoplasma gondii comprises three clonal lineages: Correlation of parasite genotype with human diseases. J Infect Dis 1995; 172: 1561-1566. https://doi.org/10.1093/infdis/172.6.1561
  3. Choi JS, Choi CS, Soh CT. Isolation of Toxoplasma gondii from congenital and acquired chorioretinitis cases. Yonsei Rep Trop Med 1980; 11: 39-42.
  4. Chung KS, Kung RN, Chung KS, Kim PK, Yun DJ, Soh CT. Congenital toxoplasmosis. Yonsei Med J 1980; 21: 62-74. https://doi.org/10.3349/ymj.1980.21.1.62
  5. Choi WY. The isolation of Toxoplasma gondii from pork and the dye test fo swine sera. J Catholic Med College 1969; 16: 229-235.
  6. Chai JY, Lin A, Shin EH, Oh MD, Han ET, Nan HW, Lee SH. Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea. Korean J Parasitol 2003; 41: 147-154. https://doi.org/10.3347/kjp.2003.41.3.147
  7. Lin A, Shin EH, Kim TY, Park JH, Guk SM, Chai JY. Genetic characteristics of the Korean isolate KI-1 of Toxoplasma gondii. Korean J Parasitol 2005; 43: 27-32. https://doi.org/10.3347/kjp.2005.43.1.27
  8. Cohen AM, Rumpel K, Coombs GH, Wastling JM. Characterization of global protein expression by two-dimensional electrophoresis and mass spectrometry: proteomics of Toxoplasma gondii. Int J Parasitol 2002; 32: 39-51. https://doi.org/10.1016/S0020-7519(01)00308-3
  9. Dlugonska H, Dytnerska K, Reichmann G, Stachelhaus S, Fischer HG. Towards the Toxoplasma gondii proteome: position of 13 parasite excretory antigens on a standardized map of two-dimensionally separated tachyzoite proteins. Parasitol Res 2001; 87: 634-637. https://doi.org/10.1007/s004360000367
  10. Ma GY, Zhang JZ, Yin GR, Zhang JH, Meng XL, Zhao F. Toxoplasma gondii: proteomic analysis of antigenicity of soluble tachyzoite antigen. Exp Parasitol 2009; 122: 41-46. https://doi.org/10.1016/j.exppara.2009.01.011
  11. Xia D, Sanderson SJ, Jones AR, Prieto JH, Yates JR, Bromley E, Tomley FM, Lal K, Sinden RE, Brunk BP, Roos DS, Wastling JM. The proteome of Toxoplasma gondii: integration with the genome provides novel insights into gene expression and annotation. Genome Biol 2008; 9: R116. https://doi.org/10.1186/gb-2008-9-7-r116
  12. Nischik N, Schade B, Dytnerska K, Dlugonska H, Reichmann G, VFischer HG. Attenuation of mouse-virulent Toxoplasma gondii parasites is associated with a decrease in interleukin-12-inducing tachyzoite activity and reduced expression of actin, catalase and excretory proteins. Microbes Infect 2001; 3: 689-699. https://doi.org/10.1016/S1286-4579(01)01425-3
  13. Lee YH, Noh HJ, Hwang OS, Lee SK, Shin DW. Seroepidemiological study of Toxoplasma gondii infection in the rural area Okcheon- gun, Korea. Korean J Parasitol 2000; 38: 251-256 https://doi.org/10.3347/kjp.2000.38.4.251
  14. Yang HJ, Jin KN, Park YK, Hong SC, Bae JM, Lee SH, Choi HS, Hwang HS, Chung YB, Lee NS, Nam HW. Seroprevalence of toxoplasmosis in the residents of Cheju island, Korea. Korean J Parasitol 2000; 38: 91-93. https://doi.org/10.3347/kjp.2000.38.2.91
  15. Lee JA, Kim DH, Kim YK, Chung EH, Choi JH, Lee HJ, Chi JG, Chai JY, Lee YH. Two cases of congenital toxoplasmosis diagnosed by polymerase chain reaction. Infect Chemother 2003; 35: 45-52.
  16. Weiss LM, Fiser A, Angeletti RH, Kim K. Toxoplasma gondii proteomics. Expert Rev Proteomics 2009; 6: 303-313.
  17. Nam HW. GRA proteins of Toxoplasma gondii: maintenance of host-parasite interactions across the parasitophorous vacuolar membrane. Korean J Parasitol 2009; 47(suppl): S29-S37. https://doi.org/10.3347/kjp.2009.47.S.S29

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