Determination of Luteolin in the Different Parts of Lithospermum erythrorhizon by HPLC

HPLC를 이용한 지치중의 부위별 Luteolin 정량

  • Han, Saem (Department of Applied Plant Science, Chung-Ang University) ;
  • Park, Jun-Yeon (Department of Applied Plant Science, Chung-Ang University) ;
  • Lee, Sul-Lim (Department of Applied Plant Science, Chung-Ang University) ;
  • Mok, So-Youn (Department of Applied Plant Science, Chung-Ang University) ;
  • Kim, Hye-Min (Department of Applied Plant Science, Chung-Ang University) ;
  • Lee, Jeong-Min (Department of Applied Plant Science, Chung-Ang University) ;
  • Ahn, Young-Hee (Department of Applied Plant Science, Chung-Ang University) ;
  • Lee, Sang-Hyun (Department of Applied Plant Science, Chung-Ang University)
  • 한샘 (중앙대학교 식물응용과학과) ;
  • 박준언 (중앙대학교 식물응용과학과) ;
  • 이설림 (중앙대학교 식물응용과학과) ;
  • 목소연 (중앙대학교 식물응용과학과) ;
  • 김혜민 (중앙대학교 식물응용과학과) ;
  • 이정민 (중앙대학교 식물응용과학과) ;
  • 안영희 (중앙대학교 식물응용과학과) ;
  • 이상현 (중앙대학교 식물응용과학과)
  • Received : 2010.08.11
  • Accepted : 2010.10.13
  • Published : 2010.10.30

Abstract

The content of luteolin in the different parts of Lithospermum erythrorhizon was determined by high performance liquid chromatography (HPLC). Luteolin was quantified by a reverse-phase column with gradient solvent program (water : ACN = 90 : 10 to 77 : 23 for 60 min). UV detection was conducted at 330 nm. The content of luteolin was measured in the seeds (0.28 mg/g), stems (0.30 mg/g), and roots (0.16 mg/g) of L. erythrorhizon. The presence of luteolin in Lithospermum species is important in agricultural crop production for increasing the amounts of clinically available medicine and health supplements.

Keywords

References

  1. Chen Z, Yang L, Zhang N, Turpin JA, Buckheit RW, Osterling C, Oppenheim JJ and Howard OM. (2003). Shikonin, a component of Chinese herbal medicine, inhibits chemokine receptor function and suppresses human immunodeficiency virus type 1. Antimicrobial Agents and Chemotherapy. 47:2810- 2816. https://doi.org/10.1128/AAC.47.9.2810-2816.2003
  2. Chung JG, Hsia TC, Kuo HM, Li YC, Lee MY, Lin SS and Hung CF. (2001). Inhibitory actions of luteolin on the growth and arylamine N-acetyltransferase activity in strains of Helicobacter pylori from ulcer patients. Toxicology in Vitro. 15:191-198. https://doi.org/10.1016/S0887-2333(01)00015-7
  3. Cho MH, Paik YS and Hahn TR. (1999). Propionylshikonin from the roots of Lithospermum erythrorhizon. Archives of Pharmacal Research. 22:414-416. https://doi.org/10.1007/BF02979068
  4. Elisa T, Maurizio LG, Santo G, Danila DM and Marco G. (2007). Citrus flavonoids: molecular structure, biological activity and nutritional properties: a review. Food Chemistry. 104:466-479. https://doi.org/10.1016/j.foodchem.2006.11.054
  5. Han J, Weng Z and Bi K. (2008). Antioxidants from a Chinese medicinal herb: Lithospermum erythrorhizon. Food Chemistry. 106:2-10. https://doi.org/10.1016/j.foodchem.2007.01.031
  6. Hu C and Kitts DD. (2004). Luteolin and luteolin-7-O-glucoside from dandelion flower suppress iNOS and COX-2 in RAW 264.7 cell. Molecular and Cellular Biochemistry. 265:107-113. https://doi.org/10.1023/B:MCBI.0000044364.73144.fe
  7. Hwang OJ, Kim YJ, Sung NS, Ahn JC, Kim SE and Hwang B. (2002). Optimization of major culture elements on growth and shikonin production in the Lithospermum erythrorhizon hairy root culture. Korean Journal of Medicinal Crop Science. 10:243-248.
  8. Ishida T and Sakaguchi I. (2007). Protection of human keratinocyes from UVB-induced inflammation using root extract of Lithospermum erythrorhizon. Biological & Pharmaceutical Bulletin. 30:928-934. https://doi.org/10.1248/bpb.30.928
  9. Jung MJ, Hu SI and Wang MH. (2008). Rat lens aldose reductase inhibitory of Taraxacum mongolicum and two Cirsium species. Journal of the Korean Society for Applied Biological Chemistry. 51:302-306. https://doi.org/10.3839/jabc.2008.047
  10. Kang JS, Ahn BZ and Blaschke G. (1998). Enantiomeric ratio of shikonin derivatives as a possible key for the determination of the origin of Lithospermi Radix. Archives of Pharmacal Research. 21:565-569. https://doi.org/10.1007/BF02975376
  11. Kim GS, Jeong TS, Kwon BM, Lee SE, Seong NS and Song KS. (2006). Inhibitory effect of acetylshikonin from Lithospermum erythrorhizon on LDL oxidation and FPTase activity. Korean Journal of Medicinal Crop Science. 14:522-523.
  12. Kuroda M, Mimaki Y, Sashida Y, Hirano T, Oka K and Dobashi A. (1997). Novel cholestane glycosides from the bulbs of Ornithogalum saundersiae and their cytostatic activity on leukemia HL-60 and MOLT-4 cell. Tetrahedron. 53:1549-1562.
  13. Lee JH, Oh MH and Lee HB. (2000). Isolation and identification of red color pigments from the Korean Lithospermum erythrorhizon. Journal of Food Science and Nutrition. 13:379- 382.
  14. Lee SH, Sohn YS, Choi YA, Lee JE, Kim DK and Lee YM. (2007). Luteolin inhibits extracellular signal-regulated kinase pathway through protease-activated receptors (-2 and -4) and their agonist activity. Natural Product Sciences. 13:169-173.
  15. Morimoto I, Kishi T, Ikegaml S and Hoirata Y. (1965). Naphtohquinone derivatives from Lithospermum erythrorhizon Siebold. et Zuccarini. Tetrahedron Letters. 52:4737-4739.
  16. Park JY, Lee S, Han S, Kim HM, Lee JM and Lee S. (2009a). A paraben derivative from the seeds of Lithospermum erythrorhizon. Journal of the Korean Society for Applied Biological Chemistry. 52:643-645. https://doi.org/10.3839/jksabc.2009.107
  17. Park JY, Lee S, Han S, Kim HM, Lee JM and Lee S. (2009b). Phytochemical constituents from the seeds of Lithospermum erythrorhizon. Natural Product Sciences. 15:181-184.
  18. Shen CC, Syu WJ, Li SY, Lin CH, Lee GH and Sun CM. (2002). Antimicrobial activities of naphthazarins from Arnebia euchroma. Journal of Natural Products. 65:1857-1862. https://doi.org/10.1021/np010599w
  19. Tabata M, Mizukami H, Hiraoka N and Konoshima M. (1974). Pigment formation in callus cultures of Lithospermum erythrorhizon. Phytochemistry. 13:927-932. https://doi.org/10.1016/S0031-9422(00)91425-0
  20. Ternai B and Markham KR. (1976). Carbon-13 NMR studies of flavonoids-1: Flavones and flavonols. Tetrahedron. 32:565-569. https://doi.org/10.1016/S0040-4020(01)93772-X
  21. Touno K, Tamaoka J, Ohashi Y and Shimomura K. (2005). Ethylene induced shikonin biosynthesis in shoot culture of Lithospermum erythrorhizon. Plant Physiology and Biochemistry. 43:101-105. https://doi.org/10.1016/j.plaphy.2005.01.004
  22. Yamasaki K, Otaka T, Mori H, Morimoto M, Ueba N, Kurokawa Y, Shiota K and Yuge T. (1993). Screening test of crude drug extract on anti-HIV activity. Yakugaku Zasshi. 113:818-824. https://doi.org/10.1248/yakushi1947.113.11_818
  23. Yingming P, Ying L, Hengshan W and Min L. (2004). Antioxidant activities of several Chinese medicine herbs. Food Chemistry. 88:347-350. https://doi.org/10.1016/j.foodchem.2004.02.002
  24. You YJ, Kim Y, Song GY and Ahn BZ. (2000). (E)-6-(1- Alkyloxyiminoalkyl)-5,8-dimethoxy-1,4-nepthoquinones: synthesis, cytotoxic activity and antitumor activity. Bioorganic & Medicinal Chemistry Letters. 10:2301-2303. https://doi.org/10.1016/S0960-894X(00)00447-9
  25. Zhang HZ, Liao MC and Guo JX. (2002). Chemical and pharmacological research progress of Chinese drug 'Zicao'. Natural Product Research and Development. 14:74-78.