Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Quantitative Determination of Bioactive Compounds in Some Artemisia capillaris by High-Performance Liquid Chromatography

  • Kim, Sang-Won (College of Pharmacy, Catholic University of Daegu) ;
  • Kim, Hyeong-Woo (College of Pharmacy, Catholic University of Daegu) ;
  • Woo, Mi-Hee (College of Pharmacy, Catholic University of Daegu) ;
  • Lee, Jae-Hyun (College of Oriental Medicine, Dongguk University) ;
  • Choi, Jae-Sue (Faculty of Food Science and Biotechnology, Pukyoung National University) ;
  • Min, Byung-Sun (College of Pharmacy, Catholic University of Daegu)
  • Received : 2010.11.10
  • Accepted : 2010.12.19
  • Published : 2010.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

In order to facilitate the quality control of Artemisia capillaris, a simple, accurate and reliable HPLC method was developed for the simultaneous determination of the six bioactive compounds: scopolin (1), chlorogenic acid (2), 2,4-dihydroxy-6-methoxyacetophenone 4-glycoside (3), hyperoside (4), isorhamnetin 3-Orobinobioside (5), and scoparone (6), which were selected as the chemical markers of A. capillaris. Separation was achieved on an Agilent Eclipse XDB-C18 column with a gradient solvent system of 0.1% trifluoroacetic acid aqueous-acetonitrile at a flow-rate of 1.0 mL/min and detected at 254 nm. All six calibration curves showed good linearity ($R^2$ > 0.998). A simple reversed phase HPLC method was developed for extracting pharmacologically active compounds scopolin, chlorogenic acid, 2,4-dihydroxy-6-methoxyacetophenone 4-glycoside, hyperoside, isorhamnetin 3-O-robinobioside, and scoparone from A. capillaris using a binary gradient of acetonitrile : 0.1% trifluoroacetic acid with UV detection at 254 nm. The scopolin (1), chlorogenic acid (2), 2,4-dihydroxy-6-methoxyacetophenone 4-glycoside (3), hyperoside (4), isorhamnetin 3-O-robinobioside (5), and scoparone (6) contents of the herb of A. capillaris collected from fifteen district markets in Korea were 0.00~0.90 mg/g, 0.06~7.29 mg/g, 0.06~0.91 mg/g, 0.07~5.05 mg/g, 0.42~13.11 mg/g, and 1.11~29.82 mg/g, respectively. The results demonstrated that this method is simple and reliable for the quality control of A. capillaris.

Keywords

References

  1. Aniya, Y., Shimabukuro, M., Shimoji, M., Kohatsu, M., Gyamfi, M.A., Miyagi, C., Kunii, D., Takayama, F., and Egashira, T., Antioxidant and hepatoprotective actions of the medicinal herb Artemisia capillaris from the Okinawa islands. Biol. Pharm. Bull. 23, 309-312 (2000). https://doi.org/10.1248/bpb.23.309
  2. Cha, J.D., Moon, S.E., Kim, H.Y., Cha, I.H., and Lee, K.Y., Essential oil of Artemisia capillaris induces apoptosis in KB cells via mitochondrial stress and caspase activation mediated by MAPK-stimulated signaling pathway. J. Food Sci. 74, T75-81 (2009). https://doi.org/10.1111/j.1750-3841.2009.01355.x
  3. Cui, C.B., Jeong, S.K., Lee, Y.S., Lee, S.O., Kang, I.J., and Lim, S.S., Inhibitory activity of caffeoylquinic acids from the aerial parts of Artemisia princeps on rat lens aldose reductase and on the formation of advanced glycation end products. J. Korean Soc. Appl. Biol. Chem. 52, 655-662 (2009). https://doi.org/10.3839/jksabc.2009.109
  4. Jang, S.I., Kim, Y.J., Lee, W.Y., Kwak, K.C., Baek, S.H., Kwak, G.B., Yun, Y.G., Kwon, T.O., Chung, H.T., and Chai, K.Y., Scoparone from Artemisia capillaris inhibits the release of inflammatory mediators in RAW 264.7 cells upon stimulation cells by interferon-$\gamma$ plus LPS. Arch. Pharm. Res. 28, 203-208 (2005). https://doi.org/10.1007/BF02977716
  5. Kim, Y.S., Bahn, K.N., Han, C.K., Gang, H.I., and Ha, Y.L., Inhibition of 7,12-dimethylbenz$[{\alpha}]$anthracene-induced mouse skin carcinogenesis by Artemisia capillaris. J. Food Sci. 73, T16-20 (2008).
  6. Logendra, S., Ribnicky, D., Yang, H., Poulev, A., Ma, J., Kennelly, E., and Raskin, I., Bioassay-guided isolation of aldose reductase inhibitors from Artemisia dracunculus. Phytochemistry 67, 1539-1546 (2006). https://doi.org/10.1016/j.phytochem.2006.05.015
  7. Mase, A., Makino, B., Tsuchiya, N., Yamamoto, M., Kase, Y., Shuichi, T., and Hasegawa, T., Active ingredients of traditional Japanese (Kampo) medicine, inchinkoto, in murine concanavalin A-induced hepatitis. J. Ethnopharmacol. 127, 742-749 (2010). https://doi.org/10.1016/j.jep.2009.11.029
  8. Okada, Y., Miyauchi, N., Suzuki, K., Kobayashi, T., Tsutsui, C., Mayuzumi, K., Nishibe, S., and Okuyama, T., Search for naturally occurring substances to prevent the complications of diabetes. II. Inhibitory effect of coumarin and flavonoid derivatives on bovine lens aldose reductase and rabbit platelet aggregation. Chem. Pharm. Bull. 43, 1385-1387 (1995). https://doi.org/10.1248/cpb.43.1385
  9. Tan, R.X., Zheng, W.F., and Tang, H.Q., Biological active substances from the genus Artemisia. Planta Med. 64, 295-302 (1998). https://doi.org/10.1055/s-2006-957438