한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제23권6호
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  • Pages.535-540
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  • 2010
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  • 1226-3591(pISSN)
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  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
권호 표지

Phenylalanine Ammonia-Lyase Gene (NtPAL4) Induced by Abiotic Stresses in Tobacco (Nicotiana tabacum)

  • Han, Woong (Department of Medical Biotechnology, College of Biomedical science, Kangwon National University) ;
  • Wang, Myeong-Hyeon (Department of Medical Biotechnology, College of Biomedical science, Kangwon National University)
  • 투고 : 2010.09.28
  • 심사 : 2010.12.16
  • 발행 : 2010.12.31
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초록

Phenylalanine ammonia-lyase (PAL), a key enzyme of the phenylpropanoid biosynthesis pathway, is activated by a number of developmental and environmental cues. The coding region of the NtPAL4 gene was 2,154 bp in length, and its deduced protein was composed of 717 amino acids. Sequence analysis of NtPAL4 cDNA from tobacco (Nicotiana tabacum L.) revealed high structural similarity to PAL genes of other plant species. The NtPAL4 gene exists as a single copy in the tobacco plant, and its transcripts were strongly expressed in flowers and leaves. NtPAL4 expression was significantly induced in response to NaCl, mannitol, and cold treatments, but it was not induced by abscisic acid (ABA). NtPAL4 expression decreased gradually after treatment with ABA and $H_2O_2$; however, NtPAL4 transcripts accumulated after treatment with methyl viologen (MV). Our results suggest that the NtPAL4 gene may function in response to abiotic stresses.

키워드

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