Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Design and Efficient Soluble Expression of a Sweet Protein, Brazzein and Minor-Form Mutant

  • Lee, Jin-Ju (Laboratory of Biomolecular Chemistry, Department of Chemistry, College of Natural Sciences, Chung-Ang University) ;
  • Kong, Ji-Na (Laboratory of Biomolecular Chemistry, Department of Chemistry, College of Natural Sciences, Chung-Ang University) ;
  • Do, Hyun-Dong (Laboratory of Biomolecular Chemistry, Department of Chemistry, College of Natural Sciences, Chung-Ang University) ;
  • Jo, Dong-Hyeon (Laboratory of Biomolecular Chemistry, Department of Chemistry, College of Natural Sciences, Chung-Ang University) ;
  • Kong, Kwang-Hoon (Laboratory of Biomolecular Chemistry, Department of Chemistry, College of Natural Sciences, Chung-Ang University)
  • Received : 2010.09.09
  • Accepted : 2010.09.27
  • Published : 2010.12.20
Download PDF
⟨ Previous Next ⟩

Abstract

Keywords

References

  1. Nabors, L. O.; Gelardi, R. C. In Alternative Sweeteners; Marcel Dekker: New York, 1991; pp 151-171.
  2. Ming, D.; Hellekant, G. FEBS Lett. 1994, 355, 106. https://doi.org/10.1016/0014-5793(94)01184-2
  3. Assadi-Porter, F. M.; Aceti, D. J.; Cheng, H.; Markley, J. L. Arch. Biochem. Biophys. 2000, 376, 252. https://doi.org/10.1006/abbi.2000.1725
  4. Assadi-Porter, F. M.; Patry, S.; Markley, J. L. Protein Expr. Purif. 2008, 58, 263. https://doi.org/10.1016/j.pep.2007.11.009
  5. Lamphear, B. J.; Barker, D. K.; Brook, C. A.; Delaney, D. E.; Lane, J. R.; Beifuss, K.; Love, R.; Thompson, K.; Mayor, J.; Clough, R.; Harkey, R.; Poage, M.; Drees, C.; Horn, M. E.; Streatfield, S. J.; Nikolov, Z.; Woodard, S. L.; Hood, E. E.; Jilka, J. M.; Howard, J. A. J. Plant Biotechnol. 2005, 3, 103.
  6. Berlec, A.; Jevnikar, Z.; Majhenic, A. C.; Rogelj, I.; Strukej, B. Appl. Microbiol. Biotechnol. 2006, 73, 158. https://doi.org/10.1007/s00253-006-0438-y
  7. Malik, A.; Rudolph, R.; Sohling, B. Protein Expr. Purif. 2006, 47, 662. https://doi.org/10.1016/j.pep.2006.02.018
  8. Choi, J. H.; Lee, S. Y. Appl. Microbiol. Biotechnol. 2004, 64, 625. https://doi.org/10.1007/s00253-004-1559-9
  9. Ying, H.; Liu, H. Immunol. Lett. 2007, 111, 66. https://doi.org/10.1016/j.imlet.2007.05.002
  10. Assadi-Porter, F. M.; Maillet, E. L.; Radek, J. T.; Quijada, J.; Markley, J. L.; Max, M. J. Mol. Biol. 2010, 398, 584. https://doi.org/10.1016/j.jmb.2010.03.017
  11. Guan, Z.; Hellekant, G.; Yan, W. Chem. Senses 1995, 20, 701.
  12. Masuda, T.; Kitabatake, N. J. Biosci. Bioeng. 2006, 102, 375. https://doi.org/10.1263/jbb.102.375
  13. Koh, J. U.; Cho, H. Y.; Kong, K. H. Bull. Korean Chem. Soc. 2007, 28, 772. https://doi.org/10.5012/bkcs.2007.28.5.772
  14. Neu, H. C.; Heppel, L. A. J. Biol. Chem. 1965, 240, 3685.
  15. Nossal, N. G.; Heppel, L. A. J. Biol. Chem. 1966, 241, 3055.
  16. Schagger, H.; Von Jagoe, G. Anal. Biochem. 1987, 166, 368. https://doi.org/10.1016/0003-2697(87)90587-2

Cited by

  1. Mutagenesis of Critical Amino Acid Residues in α-Helix and β-Sheet Structures of Brazzein vol.32, pp.11, 2011, https://doi.org/10.5012/bkcs.2011.32.11.4106
  2. Role of Lys5 Residue in β-Strand I of the Sweet-Tasting Protein Brazzein vol.40, pp.4, 2016, https://doi.org/10.1111/jfbc.12231
  3. Application of sweet and taste modifying genes for development in plants: current status and prospects vol.43, pp.4, 2016, https://doi.org/10.5010/JPB.2016.43.4.397
  4. Stable expression of brazzein protein, a new type of alternative sweetener in transgenic rice vol.45, pp.1, 2018, https://doi.org/10.5010/JPB.2018.45.1.063
  5. Importance of Glu53 in the C‐terminal region of brazzein, a sweet‐tasting protein vol.96, pp.9, 2010, https://doi.org/10.1002/jsfa.7501
  6. Improved Secretory Production of the Sweet-Tasting Protein, Brazzein, in Kluyveromyces lactis vol.64, pp.32, 2016, https://doi.org/10.1021/acs.jafc.6b02446
  7. Structural features and activity of Brazzein and its mutants upon substitution of a surfaced exposed alanine vol.131, pp.None, 2010, https://doi.org/10.1016/j.biochi.2016.09.006
  8. 감미단백질 관련 브라제인, 타우마틴 및 미라쿨린 유전자를 이용한 형질전환 상추 육성 및 발현분석 vol.45, pp.3, 2010, https://doi.org/10.5010/jpb.2018.45.3.257
  9. Functional expression of recombinant sweet-tasting protein brazzein by Escherichia coli and Bacillus licheniformis vol.33, pp.3, 2010, https://doi.org/10.1080/08905436.2019.1618323
  10. Optimized production and quantification of the tryptophan-deficient sweet-tasting protein brazzein in Kluyveromyces lactis vol.49, pp.8, 2010, https://doi.org/10.1080/10826068.2019.1621892
  11. Improved expression of recombinant sweet-tasting brazzein using codon optimization and host change as new strategies vol.34, pp.1, 2010, https://doi.org/10.1080/08905436.2019.1711113
  12. Expression of Brazzein, A Small Sweet-Tasting Protein in Saccharomyces cerevisiae: An Introduction for Production of Sweet Yeasts vol.27, pp.None, 2010, https://doi.org/10.2174/0929866527666200331134431
  13. Removal of the N ‐terminal methionine improves the sweetness of the recombinant expressed sweet‐tasting protein brazzein and its mutants in Escherichia coli vol.45, pp.3, 2021, https://doi.org/10.1111/jfbc.13354