Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Comparison of Binding Stoichiometry of [Ru(1,10-phenanthroline)2dipyrido [3,2-a:2',3'-c]phenazine]2+ and its Bis-derivative to DNA

  • Jang, Yoon-Jung (Department of Chemistry, Yeungnam University) ;
  • Lee, Hyun-Mee (Department of Chemistry, Yeungnam University) ;
  • Lee, Il-Bong (Institute of Basic Science Research, Daegu Haany University)
  • Received : 2010.07.27
  • Accepted : 2010.10.08
  • Published : 2010.12.20
Download PDF
⟨ Previous Next ⟩

Abstract

A new bis-Ru(II) complex, in which two [Ru(1,10-phenanthroline)$_2$dipyrido[3,2-a:2',3'-c]phenazine]$^{2+}$ were tethered by a 1,3-bis(4-pyridyl)propane linker, was synthesized and its binding mode and stoichiometry to DNA was investigated by optical spectroscopy including linear dichroism (LD) and fluorescence intensity measurement. The magnitude of the negatively reduced LD signal of the bis-Ru(II) complex in the dipyrido[3,2-a:2',3'-c]phenazine (DPPZ) ligand absorption region appeared to be similar compared to that in the DNA absorption region, which is considered to be a diagnostic for DPPZ ligand intercalation. The binding stoichiometry measured from its LD magnitude and enhanced fluorescence intensity corresponds to one ligand per three DNA bases, effectively violating the nearest neighbouring site exclusion model for classical DNA intercalation. This observation is in contrast with monomer analogue [Ru(1,10-phenanthroline)$_2$dipyrido[3,2-a:2',3'-c]phenazine]$^{2+}$, which is saturated at the DPPZ ligand to DNA base ratio of 0.25, or one DPPZ ligand per four nucleobases.

Keywords

References

  1. Norden, B.; Lincoln, P.; Turite, E. New York 1996, 177-252.
  2. Crothers, D. M. Biopolymers 1968, 6, 575-584. https://doi.org/10.1002/bip.1968.360060411
  3. Bond, P. J.; Langridge, R. R.; Jennette, K. W.; Lippard, S. J. Proc. Ntal. Acad. Sci. USA 1975, 72, 4825-4829. https://doi.org/10.1073/pnas.72.12.4825
  4. Hogan, M.; Dattagupta, N.; Crothers, D. M. Biochemistry 1979, 18, 280-288. https://doi.org/10.1021/bi00569a007
  5. Dupureur, C. M.; Barton, J. K. J. Am. Chem. Soc. 1994, 116, 10286-10287. https://doi.org/10.1021/ja00101a053
  6. Holmlin, R. E.; Stemp, E. D. A.; Barton, J. K. Inorg. Chem. 1998, 37, 29-34. https://doi.org/10.1021/ic970869r
  7. Choi, S.-D.; Kim, M.-S.; Kim, S. K.; Lincoln, P.; Tuite, E.; Norden, B. Biochemistry 1997, 36, 214-223. https://doi.org/10.1021/bi961675a
  8. Tuite, E.; Lincoln, P.; Nordén, B. J. Am. Chem. Soc. 1997, 119, 239-240. https://doi.org/10.1021/ja961857s
  9. Greguric, A.; Greguric, I. D.; Hambley, T. W.; Aldrich-Wright, J. R.; Collins, J. G. J. Chem. Soc., Dalton Trans. 2002, 849-855.
  10. Olson, E. J. C.; Hu, D.; Hörmann, A.; Jonkman, A. M.; Arkin, M. R.; Stemp, E. D. A.; Barton, J. K.; Barbara, P. F. J. Am. Chem. Soc. 1997, 119, 11458-11467. https://doi.org/10.1021/ja971151d
  11. Onfelt, B.; Lincoln, P.; Nordén, B.; Baskin, J. S.; Zewail, A. H. Proc. Natl. Acad. Sci. USA 2000, 97, 5708-5713. https://doi.org/10.1073/pnas.100127397
  12. Coares, C. G.; Olofsson, J.; Coletti, M.; McGarvey, J. J.; Önfelt, B.; Lincoln, P.; Nordén, B.; Tuite, E.; Matousek, P.; Parker, A. W. J. Phys. Chem. B 2001, 105, 12653-12664. https://doi.org/10.1021/jp0127115
  13. Coates, C. G.; McGarvey, J. J.; Callaghan, P. L.; Coletti, M.; Hamilton, J. G. J. Phys. Chem. B 2001, 105, 730-735. https://doi.org/10.1021/jp002856w
  14. Moon, S. J.; Kim, J. M.; Choi, J. Y.; Kim, S. K.; Lee, J. S.; Jang, H. G. J. Inorg. Biochem. 2005, 99, 994-1000. https://doi.org/10.1016/j.jinorgbio.2005.01.003
  15. Kim, J. M.; Lee, J.-M.; Choi, J. Y.; Lee, H. M.; Kim, S. K. J. Inorg. Biochem. 2007, 101, 1386-1393. https://doi.org/10.1016/j.jinorgbio.2007.05.011
  16. Hirot, C.; Lincoln, P.; Nordén, B. J. J. Am. Chem. Soc. 1993, 115, 3448-3454. https://doi.org/10.1021/ja00062a007
  17. Jang, Y. J.; Kwon, B.-H.; Choi, B.-H.; Bae, C. H.; Seo, M. S.; Nam, W. W.; Kim, S. K. J. Inorg. Biochem. 2008, 102, 1885-1891. https://doi.org/10.1016/j.jinorgbio.2008.06.012
  18. Nordén, B.; Kurucsev, T. J. Mol. Recognit. 1994, 7, 141-155. https://doi.org/10.1002/jmr.300070211
  19. Norden, B.; Kubista, M.; Kurucsev, T. Q. Rev. Biophys. 1992, 25, 51-170. https://doi.org/10.1017/S0033583500004728
  20. Lincoln, P.; Broo, A.; Nordén, B. J. Am. Chem. Soc. 1996, 118, 2644-2653. https://doi.org/10.1021/ja953363l
  21. Pierard, F.; Kirsch-De Mesmaeker, A. Inorg. Chem. Commun. 2006, 9, 111-126. https://doi.org/10.1016/j.inoche.2005.10.016
  22. Metcalfe, C.; Haq, I.; Thomas, J. A. Inorg. Chem. 2004, 43, 317-323. https://doi.org/10.1021/ic034749x