Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Effective Combination of Resistance Genes against Rice Bacterial Blight Pathogen

벼흰잎마름병 저항성 증진을 위한 유전자 조합

  • Kim, Ki-Young (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Shin, Mun-Sik (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Kim, Woo-Jae (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Mo, Young-Jun (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Nam, Jeong-Kwon (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Noh, Tae-Hwan (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Kim, Bo-Kyeong (Department of Rice and Winter Cereal Crop, NICS, RDA) ;
  • Ko, Jae-Kwon (Department of Rice and Winter Cereal Crop, NICS, RDA)
  • 김기영 (국립식량과학원 벼맥류부) ;
  • 신문식 (국립식량과학원 벼맥류부) ;
  • 김우재 (국립식량과학원 벼맥류부) ;
  • 모영준 (국립식량과학원 벼맥류부) ;
  • 남정권 (국립식량과학원 벼맥류부) ;
  • 노태환 (국립식량과학원 벼맥류부) ;
  • 김보경 (국립식량과학원 벼맥류부) ;
  • 고재권 (국립식량과학원 벼맥류부)
  • Received : 2009.08.12
  • Published : 20090900
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Abstract

This study was carried out to identify useful single gene and gene combination resistant to K1, K2, K3 and 24 bacterial blight(BB) isolates (including K3a, HB01009) breaking down Xa3 gene. Xa3, Xa4, xa5 and Xa7 genes were resistant to K1, K2, K3 of bacterial blight pathogen. Against 24 BB isolates breaking down Xa3 gene, Xa1, Xa2, xa8, Xa10, Xa11, xa13 genes were susceptible, whereas Xa4 gene was moderately resistant and xa5 and Xa21 genes were resistant. IRBB7 having Xa7 gene showed resistance responding to 24 BB isolates, whereas IRBB107 carrying Xa7 gene was susceptible to 10 BB isolates and moderately resistant to 14 BB isolates. Near-isogenic lines (NILs) of Toyonishiki and IR24, both possessing Xa7 gene, showed different resistance response against 24 BB isolates according to genetic background. Xa3+xa5, Xa4+xa5, Xa4+xa13, Xa4+Xa21, xa5+xa13, xa5+Xa21, xa13+Xa21, Xa4+xa5+xa13, Xa4+xa5+Xa21, Xa4+xa13+Xa21, xa5+xa13+Xa21, and Xa4+xa5+xa13+Xa21 were resistant to K1, K2, K3 and 24 isolates breaking down Xa3 gene. When Xa3 and xa13 genes were combined with xa5, Xa4, Xa21, resistance response was enhanced compared with single gene lines containg only Xa3 or xa13. Similarly, when Xa4 gene was combined with xa5 and Xa21, resistance response was improved by the gene combination effect.

벼흰잎마름병 저항성 유전자 Xa3을 침해하는 K3a 균계를 포함하여 24개의 균주에 대한 단일저항성 유전자와 2개 이상의 주동저항성 유전자가 결합된 계통에 대한 결과를 요약하면 다음과 같다. 1. 벼흰잎마름병 저항성 유전자 Xa3, Xa4, xa5 및 Xa7은 K1, K2, K3 균계에 저항성 반응을 보이며 K3a 균계에 대하여 Xa4는 중도저항성, xa5 및 Xa21은 저항성반응을 보였다. 2. 벼흰잎마름병 저항성 유전자 Xa3을 침해하는 24개 균주들에 대해 이병성을 보인 유전자는 Xa1, Xa2, xa8, Xa10, Xa11 및 xa13이었고, 저항성을 보인 유전자는 Xa4, xa5 및 Xa21이었다. Xa7 유전자는 반복친의 유전적 배경에 따라서 저항성 반응이 달랐다. 3. Xa4+xa5, Xa4+xa13, Xa4+Xa21, xa5+xa13, xa5+Xa21, xa13+Xa21, Xa4+xa5+xa13, Xa4+xa5+Xa21, Xa4+xa13+Xa21, xa5+xa13+Xa21, Xa4+xa5+xa13+Xa21 및 Xa3+xa5 등 2개 이상의 단일 주동 저항성 유전자가 결합되었을 때 K1, K2, K3 균계 및 Xa3 유전자를 침해하는 24개의 균주들에 강한 저항성 반응을 보였다. 4. Xa3 및 xa13 유전자는 24개의 균주들에 이병성을 보이지만 xa5, Xa4, Xa21 유전자와 결합되었을 때 단일저항성이 증가되었고, 중도저항성 반응을 보이는 Xa4 유전자는 xa5, Xa21 유전자와 결합되었을 때 고도의 저항성 반응을 보였다.

Keywords

References

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