Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
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Introduction of a Bacterial Hemoglobin Gene for Improving Bacterial Growth under Hypoxic Condition

  • Chung, Chung-Nam (Dep. of Biological Science, Dong-A University) ;
  • Yoon, Suk-Ran (Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jun, Woo-Jin (Dep. of Food and Nutrition, Chonnam National Univ.) ;
  • Shim, Sang-In (Div. of Agriculture, Gyeongsang National Univ.) ;
  • Park, In-Ho (Dep. of Biological Science, Dong-A University) ;
  • Chung, Jin-Woong (Dep. of Biological Science, Dong-A University)
  • Received : 2009.10.12
  • Accepted : 2009.12.18
  • Published : 2009.12.31

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Abstract

Using recombinant DNA technology, the vector system containing minimal fragment of a bacterial hemoglobin gene (vgb) was constructed. When this vector was inserted into Escherichia coli, the growth of the host was significantly improved in both viable cell counts and absorbance measurement, compared to that of the wild type strain. In addition, by minimizing the size of bacterial hemoglobin in the vector, the ability of vgb in growth improvement was augmented, due to the reduction of metabolic burden from the maintenance and replication of the plasmid. By using this system, it is expected that the growth of microorganisms can be improved even in the hypoxic condition.

Keywords

Acknowledgement

Supported by : Dong-A University

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