화훼연구 (FLOWER RESEARCH JOURNAL)

  • 제17권4호
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  • Pages.308-315
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  • 2009
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  • 1225-5009(pISSN)
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  • 2287-772X(eISSN)
한국화훼학회 (The Korean Society for Floricultural Science)
권호 표지

나리품종의 Flavonoid 3' Hydroxylase와 Dihydroflavonol 4-Reductase 효소학적 분석

Analysis of Flavonoid 3' Hydroxylase and Dihydroflavonol 4-Reductase Activity in Lilium Cultivars

  • 유순남 (대구가톨릭대학교 생명공학과)
  • Yu, Sun-Nam (Department of Biotechnology, Catholic University of Daegu)
  • 투고 : 2009.12.18
  • 심사 : 2009.12.24
  • 발행 : 2009.12.31

⟨ 이전 논문 다음 논문 ⟩

초록

본 실험에서 Lilium longiflorum 과 11 나리품종에 대한 생화학적, 효소학적 방법을 적용하여, 화색생합성조절 유전자 flavonoid 3'hydroxylase(F3'H)와 dihydroflavonol 4-reductase(DFR)의 활성을 분석한 결과는 다음과 같다. L. longiflorum에서 F3'H가 dihydrokaempferol(DHK) 기질을 촉매하여 dihydroquercetin(DHQ) 산물을 생성하였고, 그 외 실험에 사용한 모든 8품종에서도 F3'H 활성이 확인되었다. 나리의 F3'H는 기질특이성이 있어 naringenin(NAR)에서는 eridictiol(ERI)를 생성하지 않았다. L. longiflorum에서 DFR은 DHQ를 기질로 사용하여, leucocyanidin(LCy) 산물을 생성하였고 'Montreux', 'Monte Negro', 'Star Gazer', 'Acapulco', 'Etude', 'Le Reve'품종에서도 DFR의 활성이 나타났다. 또한 'Siberia', 'Royal Race', 'Nove Cento', 'Elite', 'Cannes' 품종에서는 DFR의 활성이 나타나지 않았다. 나리의 DFR는 기질특이성이 있어 DHK에서는 leucopelargonidin(LPg)를 생성하지 않았다.

The activities of flower color biosynthesis-controlling enzymes, flavonoid 3'hydroxy lase (F3'H) and dihydroflavonol 4-reductase (DFR), were analyzed in Llium longijlorum and 11 lily cultivars using biochemical and enzymological methods. Dihydroquercetin (DHQ) product was synthesized by F3'H using dihydrokaempferol (DHK) as a substrate in Lilium longiflorum. F3'H activity was also detected in the eight cultivars tested. The substrate-specific activity of F3'H was observed because eridictiol (ERI), which uses naringenin (NAR) as a substrate, was not detected in the tested cultivars. Leucocyanidin (LCy) product was synthesized by DFR using DHQ as a substrate in Lilium longiflorum. DFR activity was also detected in 'Le Reve', 'Montreux', 'Monte Negro', 'Etude', 'Acapulco', and 'Star Gazer', but not in 'Siberia', 'Royal Race', 'Nove Cento', 'Elite', and 'Cannes'. Substrate-specific activity of DFR was observed because leucopelargonidin (LPg), which uses DHK as a substrate, was not detected in the tested cultivars.

키워드

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