대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지

향부자(香附子)가 천식 관련 Th1/Th2 세포 관련 cytokine 분비에 미치는 영향

Studying of the Effects of Cyperus rotundus L. extract on Th1/Th2 Cell-derived Cytokines

  • 염종훈 (경희대학교 한의과대학 폐계내과학 교실) ;
  • 이형구 (경희대학교 한의과대학 폐계내과학 교실) ;
  • 정승기 (경희대학교 한의과대학 폐계내과학 교실) ;
  • 정희재 (경희대학교 한의과대학 폐계내과학 교실)
  • Yom, David Jong-Hoon (Division of Allergy & Repiratory System, Dept. of Internal Medicine College of Oriental Medicine, Kyung-Hee University) ;
  • Rhee, Hyung-Koo (Division of Allergy & Repiratory System, Dept. of Internal Medicine College of Oriental Medicine, Kyung-Hee University) ;
  • Jung, Sung-Ki (Division of Allergy & Repiratory System, Dept. of Internal Medicine College of Oriental Medicine, Kyung-Hee University) ;
  • Jung, Hee-Jae (Division of Allergy & Repiratory System, Dept. of Internal Medicine College of Oriental Medicine, Kyung-Hee University)
  • 발행 : 2009.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Background and Objective : Cyperus rotundus L. (CR) is a commonly used herbal medicine in Asian countries such as Korea, China and Japan. The present study was designated to evaluate the direct effects of CR on helper T cell activities and on Th1/Th2 lineage development in vitro. Materials and Methods : Spleen cells from 8 week BALB/c mice were cultured in CR extracts containing medium without activation for 24 hours and with activation for 48 hours. CD4+ T cells were isolated and analyzed for mRNA expression levels of INF-$\gamma$, IL-4, T-bet and GATA-3 by RT-PCR and secretion cytokines levels of INF-$\gamma$, IL-4, IL-5 and IL-10 by ELISA. Results : The results demonstrated that CR had no mitogenic effects on unstimulated CD4+ T cells, but augmented CD4+ T-cell proliferation upon activation with anti-CD3/anti-CD28 antibodies in a dose-dependent manner. CR treatment significantly increased CD4+ T cell population and the IFN-$\gamma$ expression was significantly enhanced, while IL-4 expression was significantly decreased. In addition, in vitro Th1/Th2 polarization experiments revealed that CR enhanced IFN-$\gamma$ secretion in Th1 cells, but reduced the IL-4 in Th2 cells in a dose-dependent manner. Conclusion : These results suggest that CR treatment could be a desirable alternative therapy for the prevention or correction of Th2 dominant pathological disorders, such as allergy and asthma.

키워드

참고문헌

  1. 이상인, 안덕균, 신민교, 노승현, 이영종, 이선희. 한약임상응용. 서울. 전통의학연구소: 1986, p.231-2.
  2. 박동희, 이형구, 정승기, 정희재. High through screening(HTS)를 이용한 백서의 비장세포에서의 IL-5 분비조절 한약물 Screening. 대한한방내과학회지. 2006:27(2):394-406.
  3. 전국한의과대학 폐계내과학교실. 동의폐계내과학. 서울: 도서출판국진: 2004, p. 321-2, 329-31.
  4. Mosmann TR, Cherwinski H, Bond MW, Giedlin MA, Coffman RL. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 1986:136(7):2348-57.
  5. 白東鎭, 鄭熙才, 李珩九, 鄭昇杞: 解表二陳湯加減方이 Asthma model 內의 Cytokine에 미치는 影響, 大韓韓醫學會誌. 2000:21(3):3-13.
  6. 정진용, 정희재, 정송기, 이형구. 小靑龍湯과 小靑龍湯加沙參이 BEAS-2B 人間 氣管支上皮細胞의 IL-6, IL-8 및 GM-CSF mRNA level에 미치는 影響. 대한한의학회지. 2003:24(1):74-83.
  7. Heo TS, Jung HJ, Kim HY, Jung SK, Rhee HK: The Effects of Sabaek-San and Glycyrrhizae Radix on IL-4, IL-5 and IL-6 in asthma model. Journal of Oriental Medicine. 2000:5(1):1-13.
  8. 鄭旭, 鄭熙才, 鄭昇杞, 李珩九: 杏仁과 桔梗이 Asthma model 內의 Cytokine IL-4, IL-5, IL-6에 미치는 影響, 大韓韓方內科學會誌. 2000:21(1):31-8.
  9. 황우석, 최준용, 이재성, 주창엽, 정희재, 이형구, 정승기. 청상보하탕의 기관지천식환자에 대한 스테로이드 절약효과. 대한한방내과학회지, 2003:24(1):1-10.
  10. 최준용, 이재성, 황우석, 정희재, 이형구, 정승기 가미패모탕의 기관지천식환자에 대한 객담배출 용이도에 따른 임상적 효과. 대한한방내과학회지. 2003:24(3):588-96.
  11. 황우석, 최준용, 이재성, 정희재, 이형구, 정승기. 기관지천식환자에서 가미패모탕치료에 따른 IgE. 호산구 및 cytokine의 변화. 대한한방내과학회지. 2003:24(4):747-58.
  12. 허태석, 고은정, 정희재, 이형구, 배현수, 정승기. 加味淸上補下場의 물과 에탄올 추출법에 따른 抗알레르기 效果에 對한 硏f究. 대한한방내과학회지. 2006:27(1):40-54.
  13. Leckie MJ, ten Brinke A, Khan J, Diamant Z, O'Connor BJ, Walls CM, Mathur AK, Cowley HC, Chung KF, Djukanovic R, Hansel TT, Holgate ST, Sterk PJ, Barnes PJ. Effects of an interleukin-5 blocking monoclonal antibody on eosinophils, airway hyper-responsiveness, and the late asthmatic response. Lancet. 2000:355:2144-8.
  14. Busse WW, et al. Allergy principles & practice. 5th ed. St. Louise: Mosby: 1998, p. 838-58.
  15. 천식연구회, 한국보건사회연구원. 한국인 성인에서 천식양 증상의 빈도. 대한내과학회지. 2001:60:196-205.
  16. 이영선, 한옥경, 신상우, 박종현, 권영규. 香附子 열수추출물의 Nitric oxide 생성 및 iNOS 유전자 발현에 미치는 영향. 동의생리병리학회지. 2003:17(3):771-6.
  17. 김동철, 백승희, 박종현, 이영선. 香附子가 자궁 근종세포의 성장억제와 MAP Kinase 활성 및 Cell Apoptosis 에 미치는 영향. 대한한방부인과학회지. 2003:16(2):18-33.
  18. 김선기, 황방연, 강신정, 이정준, 노재섭, 이경순. 香附子의 성분 및 Nitric Oxide 생합성 저해효과. 생약학회지. 2000:31(1):1-6.
  19. Mosmann TR, Sad S. The expanding universe of T-cell subsets: Th1, Th2 and more. Immunol. 1996:17:138-46.
  20. Boushey HA, Fahy JV. Targeting cytokines in asthma therapy: round one. Lancet. 2000:356:2114-6. https://doi.org/10.1016/S0140-6736(00)03486-3
  21. Hansen G, Berry G, DeKruyff RH, Umetsu DT. Allergen-specific Th1 cells fail to counterbalance Th2 cell-induced airway hyperreactivity but cause severe airway inflammation. J Clin Invest. 1999;103(2):175-83. https://doi.org/10.1172/JCI5155
  22. Cembrzynska-Nowak M, Szklarz E, Inglot AD, Teodorczyk-Injeyan JA. Elevated release of tumor necrosis factor-alpha and interferon-gamma by bronchoalveolar leukocytes from patients with bronchial asthma. Am Rev Respir Dis. 1993;147(2):291-5. https://doi.org/10.1164/ajrccm/147.2.291
  23. 최준용, 정희재, 이재성, 조일현, 이건영, 정승연, 이형구, 정승기. 가미청상보하탕과 Th2 가설-미완의 천식이론. 2004 대한한방내과학회 춘계학술대회 논문집. 2004;28-40.
  24. Magnan AQ, Mely LG, Camilla CA, Badier MM, Montero-Julian FA, Guillot CM, Casano BB, Prato SJ, Fert V, Bongrand P, Vervloet D. Assessment of the Th1/Th2 paradigm in whole blood in atopy and asthma. Increased IFN-gamma-producing CD8(+) T cells in asthma. Am J Respir Crit Care Med. 2000;161(6):1790-6. https://doi.org/10.1164/ajrccm.161.6.9906130
  25. Flood-Page PT, Menzies-Gow AN, Kay AB, Robinson DS. Eosinophil's role remains uncertain as anti-interleukin-5 only partially depletes numbers in asthmatic airway. Am J Respir Crit Care Med. 2003;167(2):199-204. https://doi.org/10.1164/rccm.200208-789OC