한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)

  • 제26권3호
  • /
  • Pages.269-278
  • /
  • 2009
  • /
  • 1225-9098(pISSN)
  • /
  • 2288-1069(eISSN)
한국응용과학기술학회 (The Korean Society of Applied Science and Technology)
권호 표지
DOI QR코드

DOI QR Code

광대싸리 줄기 추출물의 항산화 활성

Antioxidative Activity of Securinega suffruticosa Extract

  • 박수남 (서울산업대학교 자연생명과학대학 정밀화학과) ;
  • 김재현 (서울산업대학교 자연생명과학대학 정밀화학과) ;
  • 안유진 (서울산업대학교 자연생명과학대학 정밀화학과)
  • Park, Soo-Nam (Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Technology) ;
  • Kim, Jai-Hyun (Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Technology) ;
  • Ahn, You-Jin (Department of Fine Chemistry, College of Nature and Life Science, Seoul National University of Technology)
  • 발행 : 2009.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In this study, the anti oxidative effects, inhibitory effects on elastase, and components of Securinega suffruticosa extracts were investigated. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities ($FSC_{50}$) and reactive oxygen species ROS) scavenging activities ($OSC_{50}$) of extract/fractions from Securinega suffruticosa were measured. The aglycone fraction ($9.04\;{\pm}\;0.51\;{\mu}g/mL$) and 50 % ethanol extract ($1.05\;{\pm}\;0.41\;{\mu}g/mL$) showed the most effective scavenging activities. The protective effects on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The aglycone fraction exhibited the most prominent cellular protective effect (${\tau}_{50},\;102.77\;{\pm}\;5.37$ min at $10{\mu}g/mL$). The inhibitory effect of ethyl acetate fraction on tyrosinase were of examined ($210.0\;{\pm}\;1.02\;{\mu}g/mL$). The inhibitory effect of aglycone fraction on elastase were also investigated ($17.6\;{\pm}\;1.26\;{\mu}g/mL$). These results indicate that extract/fractions of Securinega suffruticosa can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS. Securinega suffruticosa extract could be used as a new cosmeceutical ingredient for whitening and anti-wrinkle products.

키워드

참고문헌

  1. K. J A. Davies, Protein Damage and Degradation by Oxygen Radical, J BioI. Chem, 262, 9895 (1987)
  2. I. C. Kim, Antioxidative Properties and Whitening Effects of the Polyconi Muliflori Radix, Polygonati Rhizorna and Ephedrae Herba, J the Korean Oil Chemists Soc., 25(4), 533 (2008)
  3. C. S. Foote, Photosensitized Oxidation and Singlet Oxygen; Consequences in Biological Systems, ed. W. A. Pryor, 2, 85, Acdemic press, New York (1976)
  4. S. N. Park, Ph. D. Dissertation, Seoul National Univ., Seoul, Korea (1989)
  5. S. N. Park, Skin Aging and Antioxidant, J Soc. Cosmet. Scientists Korea, 23, 75 (1997)
  6. S. N. Park, Protective Effect of Isoflavone, Genistein from Soybean on Singlet Oxygen Induced Photohemolysis of Human Erythrocytes, Korean J Food Sci. Technol., 35(3), 510 (2003)
  7. S. N. Park, Antioxidative Properties of Baicalein, Component from Scutellaria baicalensis Georgi and Its Application to Cosmetics (I), J, Korean Ind, Eng, Chem., 14(5), 657 (2003)
  8. K. Scharffetter-Kochanek, Photoaging of the Connective Tissue of Skin: Its Prevention and Therapy, Antioxidants in Disease Mechanism and Therapy, ed. H.Sies, 38, 639 (1997)
  9. R. M. Tyrrell and M. Pidoux, Singlet Oxygen Involvement in the Inactivation of Cultured Human Fibroblast by UV A and Near Visible Radiations, Photochem Photobiol., 49, 407 (1989) https://doi.org/10.1111/j.1751-1097.1989.tb09187.x
  10. G. F. Vile and R. M. Tyrrell, UV A Radiation-Induced Oxidative Damage to Lipid and Protein In Vitro and in Human Skin Fibroblasts is Dependent on Iron and Singlet Oxygen, Free Radical Biology & Medicine, 18, 721 (1995) https://doi.org/10.1016/0891-5849(94)00192-M
  11. K. Scharffetter-Kochanek, M. Wlaschek, K. Briviba, and H. Sies, Singlet Oxygen Induces Collagenase Expression in Human Skin Fibroblasts, .FEJ3S Lett., 331, 304 (1993)
  12. M. Wlaschek, K. Briviba, G. P. Stricklin, H. Sies, and K. Scharlfetter-Kochanek, Singlet Oxygen may Mediate the Ultraviolet A in Induced Synthesis of Interstitial Collagenase, J Invest. Dennatol., 104, 194 (1995) https://doi.org/10.1111/1523-1747.ep12612751
  13. A. Oikarinen, J. Karvonen, J. Uitto, and M. Hannuksela, Connective Tissue Alterations in Skin Exposed to Natural and Therapeutic UV -radiation, Photodennatology, 2, 15 (1985)
  14. A. Oikarinen and M. Kallioinen, A Biochemical and Immunohistochemical Study of Collagen in Sun-exposed and Protected Skin, Photodermatology, 6, 24 (1989)
  15. L. H. Kligman, UV A Induced Biochemical Changes in Hairless Mouse Skin Collagen: a Contrast to UVB Effects, ed. F. Urbach, 209, Valdemar, Overland Park (1992)
  16. J. W. Choi, S. I. Kim. J. Y. Kim, H. J. Yang, K. H. Lee,and S. N. Parkang, K. H. tive and Cellualr Protective Effects of Jeju Native Plant Extracts Against Reactive Oxygen Species (I). J. Soc. Cosmet. SciJ . Ysts Korea, 32(3), 181(2006)
  17. H. J. Yang and S. N. Park, Evaluation of Antioxidant Potential of Extract/fractions of Equisetum arense (I), J. Soc. Cosmet. Scientists Korea, 33(2), 61(2007)
  18. H. J. Yang and S. N. Park, Component Analysis and Study on Anti-elastase Activity of Equisetum arese (II), J. Soc. Cosmet. Scientists Korea, 33(3), 139(2007)
  19. S. M. Jeon, S. I. Kim, J. Y. Ahn, and S. N. park, Antioxidtive Potenties of Extract/fractions of Suaeda asparagoides and Salicornia herbacea Extracts (I), J. Soc. Cosmet. Scientists Korea, 33(3), 145(2007)
  20. Z. Horii, M. Ikeda, M. Hanaoka, M. Yamauchi, and Y. Tamura, Structure of Securitinine, Chem. Pharm. Bull, 15, 1633(1967) https://doi.org/10.1248/cpb.15.1633
  21. S. G. Yunosova. S. D. Gusakova, and A. I. Glushenkova, A Comparative Investigation of the Fatty Acid Compositions of the seeds of a Number of Lines of a Genetic Collection of Securinega suffruticosa, Khim. Prir. Soedin, 3, 277(1986)
  22. V. Snieckus, The Alkaloids: Chemistry and Physiology, Ed by R. H. F. Manske Academic press, New York, 14, 425 (1973)
  23. J. A. Beutler, E. W. Karbon, A. N. Brubaker, R. Malik, D. R. Curtis, and S. j. Enna, Securinine Alkaloids: a New Class of GABA Receptor Antagonist, Brain Research, 330, 135 (1985)
  24. J. S. Eun, H. W. Park, S. M. Seo, J. H. Yang, K. H. Leem, S. H. Oh, C. H. Oh, N. I. Baek, and D. K. Kim, An Acetylcholinesterase Inhibitor from the Leaves of Securinega suffruticosa, Kor. J Pharamacogn., 34(1), 14 (2003)
  25. S. C. Lee, Chemical Studies of Tannin and Related Compounds from the Leave of Securinega suffruticosa, a collection of learned Pharm. papers, Chung-Buk Univ., 7, 9, (1992)
  26. S. C. Lee. B. T. Ahn, W. Y. Park, S. H. Lee, J. S. Ro, and K. S. Lee, Chemical Study on the Phenolic Compounds from the Leave of Securinega suffruticosa, Kor. J. Pharamacogn., 25(2), 105 (1994)
  27. S. C. Lee, B. T. Ahn, W. Y. Park, S. H. Lee, J. S. Ro, and K. S. Lee, Phenolic Compounds from Stems of Securinega suffruticosa, Kor. J. Pharamacogn., 27(1), 1 (1996)
  28. J Y. Kim, H. J Yang, K. H. Lee, S. M. jeon, Y. j. Ahn, B. R. Won, and S. N. Park, Antioxidative and Antiagiang Effect of jeju Native Plant Extracts (II), J Soc. Cosmet. Scientists Korea, 33(3), 165 (2007)