Korean Journal of Biological Psychiatry (생물정신의학)

The Korean Society of Biological Psychiatry (대한생물정신의학회)
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Chronic Treatment of Fluoxetine Increases Expression of NCAM140 in the Rat Hippocampus

장기간 플루세틴 처리에 의한 흰쥐 해마에서의 NCAM140 유전자 발현의 증가

  • Choi, Mi Ran (Division of Molecular & Life Sciences, Hanyang University) ;
  • Chai, Young Gyu (Division of Molecular & Life Sciences, Hanyang University) ;
  • Jung, Kyoung Hwa (Division of Molecular & Life Sciences, Hanyang University) ;
  • Baik, Seung Youn (Division of Molecular & Life Sciences, Hanyang University) ;
  • Kim, Seok Hyeon (Department of Neuropsychiatry, College of Medicine & Institute of Mental Health, Hanyang University) ;
  • Roh, Sungwon (Department of Neuropsychiatry, College of Medicine & Institute of Mental Health, Hanyang University) ;
  • Choi, Joonho (Department of Neuropsychiatry, College of Medicine & Institute of Mental Health, Hanyang University) ;
  • Lee, Jun-Seok (Department of Neuropsychiatry, College of Medicine, Kwandong University) ;
  • Choi, Ihn Geun (Department of Neuropsychiatry, College of Medicine, Hallym University) ;
  • Yang, Byung-Hwan (Department of Neuropsychiatry, College of Medicine & Institute of Mental Health, Hanyang University)
  • 최미란 (한양대학교 분자생명과학부) ;
  • 채영규 (한양대학교 분자생명과학부) ;
  • 정경화 (한양대학교 분자생명과학부) ;
  • 백승연 (한양대학교 분자생명과학부) ;
  • 김석현 (한양대학교 의과대학 신경정신과학교실 및 정신건강연구소) ;
  • 노성원 (한양대학교 의과대학 신경정신과학교실 및 정신건강연구소) ;
  • 최준호 (한양대학교 의과대학 신경정신과학교실 및 정신건강연구소) ;
  • 이준석 (관동대학교 의과대학 신경정신과학교실) ;
  • 최인근 (한림대학교 의과대학 신경정신과학교실) ;
  • 양병환 (한양대학교 의과대학 신경정신과학교실 및 정신건강연구소)
  • Published : 2009.02.28
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Abstract

Objectives : Most of the mechanisms reported for antidepressant drugs are the enhancement of neurite outgrowth and neuronal survival in the rat hippocampus. Neural cell adhesion molecule 140(NCAM140) has been implicated as having a role in cell-cell adhesion, neurite outgrowth, and synaptic plasticity. In this report, we have performed to elucidate a correlation among chronic antidepressant treatments, NCAM140 expression, and activation of phosphorylated cyclicAMP responsive element binding protein(pCREB) which is a downstream molecule of NCAM140-mediated intracellular signaling pathway in the rat hippocampus. Methods : Fluoxetine(10mg/kg) was injected acutely(daily injection for 5days) or chronically(daily injection for 14days) in adult rats. RNA and protein were extracted from the rat hippocampus, respectively. Real-time RT-PCR was performed to analyze the expression pattern of NCAM140 gene and western blot analyses for the activation of the phosphorylation ratio of CREB. Results : Chronic fluoxetine treatments increased NCAM140 expression 1.3 times higher than control in rat hippocampus. pCREB immunoreactivity in the rat hippocampus with chronic fluoxetine treatment was increased 4.0 times higher than that of control. Conclusion : Chronic fluoxetine treatment increased NCAM140 expression and pCREB activity in the rat hippocampus. Our data suggest that NCAM140 and pCREB may play a role in the clinical efficacy of antidepressants promoting the neurite outgrowth and neuronal survival.

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