Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Genetic Variations and Phylogenetic Relationship of and Pueraia lobata Ohwi (Fabaceae) and Related Taxa by RAPD Makers

RAPD분자마커를 이용한 칡(콩과) 및 근연분류군의 유전적 변이 및 유연관계

  • Kim, Dong-Kap (Department of Life Science, Kyungwon University) ;
  • Jang, Dae-Sik (Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine) ;
  • Kim, Jin-Sook (Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine) ;
  • Kim, Joo-Hwan (Department of Life Science, Kyungwon University)
  • 김동갑 (경원대학교 생명과학과) ;
  • 장대식 (한국한의학연구원 한의융합연구본부 당뇨합병증연구센터) ;
  • 김진숙 (한국한의학연구원 한의융합연구본부 당뇨합병증연구센터) ;
  • 김주환 (경원대학교 생명과학과)
  • Published : 2009.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

RAPD analyses were performed to investigate genetic relationships and useful molecular maker for 3 species and their 17 regional populations of the Pueraria lobata and related taxa. The length of amplified DNA fragments ranged from 200 to, 2,800 bp. Two hundred and eight scorable polymorphic makers and three scorable monomorphic makers were found from the PCR reactions with 15 random oligo primers, and those were analyzed by Nei's genetic distance coefficient. Based on the UPGMA phenogram from RAPD analyses, two major groups (9 populations from Korea; 3 populations from foreign countries) were recognized. And it showed distinct genetic differences from related taxa. The RAPD results was very useful to define the samples by geographical distribution and to discuss the relationships among the populations and their related taxa of the Pueraria lobata.

동아시아에 분포하는 콩과에 속하는 칡 1종의 13집단과 근연분류군 2종의 4집단 등 총 17개의 개체군에 대하여 유전적 유연관계 및 종간 특이적인 분류학적으로 유용한 분자마커를 알아보기 위해 RAPD 분석을 실시하였다. 15개의 oligo primer를 이용한 효소중합반응을 통해 증폭된 RAPD 절편들은 200 bp에서 2,800 bp 사이의 구간에서 관찰되었다. 이중 유효한 polymorphic band makers는 총 208개, monomorphic bands는 3개를 확인하였으며, 칡의 종 특이적 분자마커는 4개로 확인되었다. 이러한 자료에 근거하여 칡속의 17개 개체군 집단에 대한 UPGMA 분석을 실시하였다. 도출된 UPGMA phenogram에서 한국산 칡 9개체군과 국외산 칡 3개체군이 각각 독립적인 두 개의 작은 유집군을 형성하였으며, 이후 두 유집군이 하나로 크게 유집되어 다른 칡 근연분류군과는 뚜렷하게 구분되었다. 따라서 RAPD 분석은 칡과 근연분류군간의 유연관계 분석 및 한국산과 국외산 집단의 원산지판별에 매우 유용한 분자마커로 생각된다.

Keywords

References

  1. Chang, S.-M., S.-H. Noh and S.D. Park. 1999. Herbal resources plant. Hakmunsa. Seoul. (in Korean)
  2. Doyle, J.J. and J.L. Doyle. 1987. A rapid DNA isolation methods for small quantities of fresh tissues. Phytochemical Bulletin. 17: 144-163
  3. Fu, C., Y. Qiu and H. Kong. 2003. RAPD analysis for genetic diversity in Changium smyrnioides (Apiaceae), an endangered plant. Bot. Bull. Acad. Sin. 44: 13-18
  4. Junei, K., F. Juncihi, B. Junko, T. Takashi and Y. Masaki. 1987. Studies on the consti-tuents of Pueraria lobata. III. Chem. Pharm. Bull. 35: 4846-4850 https://doi.org/10.1248/cpb.35.4846
  5. Kang, I.H., J.H. Cho, D.H. Kim, Y.H. Shin, E.K. Kim, J.W. Kim, W.K. Hwang and H.Y. Choi. 2002. Monitoring on Endocrine Disruptors in Natural Medicines-Studies on Pesticide Residues in Natural Medicines. Kor. J. Herbology 17: 175-182. (in Korean)
  6. Kim, B.M. and S.H. Yoon. 1988. Separation and Properties of crude $\beta$-amylase from Pueraria thunbergiana Bentham. Chung-ang Journal of Natural Science 2: 43-51. (in Korean)
  7. Koichi, T. and I. Hideji. 1982. Isoflavonoids and the other constituents in callus tissues of Pueraria lobata. Chem. pharm. Bull. 30: 1496-1499 https://doi.org/10.1248/cpb.30.1496
  8. Lee, K.S., K.Y. Yim, W.Y. Choi and M.J. Oh. 1985. Isolation and characterization of arrowroot leaf proteins. Korean J. Food & Nutr. 14: 345-352. (in Korean)
  9. Lee, S.W., D.K. Kim, H.C. Shin and J.H. Kim. 2005. Genetic variations and relationships of Neofinetia falcata (Thunb.) Hu (Orchidaceae), a rare and endangered species in Korea. The 60th Annual Meeting of the Korean Association of Biological Sciences, vol. 9 Supplement
  10. Ministry of health and social affairs. 1987. The Korea Pharmacopoeia 5 edit. Korea Medical Index. Seoul. (in Korean)
  11. Nei, M. 1972. Genetic distance between populations. Amer. Natur. 106: 283-292 https://doi.org/10.1086/282771
  12. Nybom, H. 1993. Applications of DNA fingerprinting in plant population studies in DNA fingerprinting: State of the Science, Burke, Dolf, T.G., A.J. Jeffreys and R. Wolff, Eds., Birkh user, Basal. pp. 293-309
  13. Rohlf, F.J. 1989. NTsys-PC Numerical taxonomy and multivariat analysis systeme, version 1.50. Exeter pubi. New York
  14. Sim, S.Y. 1983. Medicinal natural food. Changjosa. Seoul. (in Korean)
  15. Society for the research of pharmacognosy. 1998. Modern pharmacognosy. Hakchangsa. Seoul. (in Korean)
  16. Stiles, J.I., C. Lemme, S. Sondur, M.B. Morshildi and R. Manshardt. 1993. Using randomly amplified polymorphic DNA for evaluating genetic relationships among papaya cultivars. Theor. Appl. Genet. 85: 697-701 https://doi.org/10.1007/BF00225007
  17. Tae, K.H., D.K. Kim and J.H. Kim. 2005. Analysis of phylogenetic relationship among Korean Pinellia Tenore (Araceae) using RAPD markers. Kor. J. Plant Tax. 35: 161-174. (in Korean) https://doi.org/10.11110/kjpt.2005.35.3.161
  18. Tae, K.H., D.K. Kim and J.H. Kim. 2005. Genetic Variations and Phylogenetic Relationships of Tribe Forsythieae (Oleaceae) Based on RAPD Analysis. Korean J. Plant Res. 8: 135-144
  19. Takashi, S., Akihiko K., Akira T., Yoshio T. and Tatsuzo F. 1992. Mechanism o fantioxidants action of Pueraria glycoside (PG)-1 (an isoflavonoids) and mangiferin (a Xanthonoid). Chem. Pharm. Bull. 40: 721-724 https://doi.org/10.1248/cpb.40.721
  20. Williams, J.G.K, A.R. Kubelik, K.J. Livark, J.A. Rafalski and S.V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic makers. Nucl. Acids. Res. 18: 6531-6535 https://doi.org/10.1093/nar/18.22.6531
  21. Yang, S.K. and Y.S. Ko. 1994. Constituent of Pueraria mirifica root juice and Pueraria mirifica root extract. The Journal of Korean living science research. 12: 25-45. (in Korean)
  22. Yang, Y.W., W.H.J. Kuo and T.H. Wong. 1998. Genetic polymorphism of seven populations of Capsella bursapastoris based on RAPD markers. Bot. Bull. Acad. Sin. 39: 17-21
  23. Yuk, C.S. 1989. Coloured medicinal plants of Korea. Academybook. Seoul. (in Korean)