Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
권호 표지

The Glucosinolate and Sulforaphane Contents of Land Race Radish and Wild Race Radish Extracts and Their Inhibititory Effects on Cancer Cell Lines

재래종 무와 갯무 추출물의 암세포주 증식 저해 활성 및 Glucosinolate와 Sulforaphane의 함량

  • Choi, Sun-Ju (Dept. of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Choi, A-Reum (Dept. of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Cho, Eun-Hye (Dept. of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Kim, So-Young (Dept. of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University) ;
  • Lee, Gun-Soon (Dept. of Living Sciences, Korea National Agriculture College) ;
  • Lee, Soo-Seong (BioBreeding Institute) ;
  • Chae, Hee-Jeong (Dept. of Food and Biotechnology, and Center for Food Function and Safety, Hoseo University)
  • 최선주 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 최아름 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 조은혜 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 김소영 (호서대학교 식품생물공학과 및 식품기능안전연구센터) ;
  • 이건순 (한국농업대학 교양공통학부) ;
  • 이수성 ((주)바이오브리딩연구소) ;
  • 채희정 (호서대학교 식품생물공학과 및 식품기능안전연구센터)
  • Received : 2009.02.16
  • Accepted : 2009.04.20
  • Published : 2009.08.31
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Abstract

The inhibitory effects of land race radish (LRR) and wild race radish (WRR) extracts on cancer cell lines were investigated. A and their glucosinolate and sulforaphane contents were analyzed. The anticancer activitiesy of the LRR and WRR extracts on the breast cancer cell line MCF-7 were determined by a CCK (cell counting kit) assay, in which WWR showed higher inhibition rates than LRR. The sulforaphane content of WRR was higher than that of LRR. In the lung cancer cell line, A-549, WRR showed higher inhibition rates and a higher total glucosinolate content than LRR. The glucosinolate contents of the radishes were analyzed by the Pd-quicktest method, showing that WRR contained more glucosinolate than LRR in both the trunk and root. In conclusion, these results indicate that wild race radish could be used for the quality improvement of radishes.

무의 유방암 및 폐암 세포주에 대한 증식 저해 활성을 분석하였고, 항암 관련 지표 물질로 알려진 glucosinolate의 함량 및 sulforaphane의 함량을 조사하였다. 유방암 세포주의 하나인 MCF-7 세포주를 이용한 CCK(cell counting kit) assay분석법을 사용하여 재래종 무와 갯무 추출물의 세포 증식 저해율을 조사한 결과, 재래종 무보다 갯무의 세포 증식 저해율이 높았으며, sulforaphane 함량에서도 갯무가 재래종 무보다 높은 것으로 나타났다. 폐암 세포주의 하나인 A-549 세포주를 이용한 세포 증식 저해율을 조사한 결과, 갯무가 재래종 무보다 높은 세포 증식 저해율을 보였으며, 총 glucosinolate함량에서도 갯무가 재래종 무보다 높은 것으로 나타났다. 본 연구를 통하여 갯무를 이용하여 재래종 무의 품질 특성을 개량할 수 있는 가능성을 제시하였다.

Keywords

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