International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Swelling-activated $Cl^-$ Channels in Human Salivary Gland Acinar Cells

  • Chung, Ge-Hoon (Department of Physiology, School of Dentistry, Seoul National University and Dental Research Institute) ;
  • Sim, Jae-Hyun (Department of Physiology, School of Dentistry, Seoul National University and Dental Research Institute) ;
  • Kim, Soung-Min (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University and Dental Research Institute) ;
  • Lee, Jong-Ho (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University and Dental Research Institute) ;
  • Chun, Gae-Sig (Department of Oral Physiology, School of Dentistry, Dankook University) ;
  • Choi, Se-Young (Department of Physiology, School of Dentistry, Seoul National University and Dental Research Institute) ;
  • Park, Kyung-Pyo (Department of Physiology, School of Dentistry, Seoul National University and Dental Research Institute)
  • Published : 2009.09.30
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Abstract

The role of $Cl^-$ channels in regulatory volume decrease (RVD) in human salivary gland acinar cells was examined using a whole-cell patch clamp technique. Human tissues were obtained from healthy volunteers or from patients with oromaxillofacial tumors. During the measurements, $K^+$-free solutions were employed to eliminate contamination of whole-cell conductance by $K^+$ currents. When the cells were exposed to a 70% hypotonic solution, outward-rectifying currents, which were not observed in the resting state, were found to have significantly increased both in human labial and parotid gland acinar cells. The amplitudes of the currents were reduced in a low $Cl^-$ bath solution. Furthermore, the addition of $100{\mu}M$ 5-Nitro-2- (3-phenyl propylamino) benzoic acid (NPPB) or $100{\mu}M$ 4,4'-diisothio cyanatostilbene-2,2'-disulphonic acid (DIDS), known to partially block $Cl^-$ channels, significantly inhibited these currents. Its outward-rectifying current profile, shift in reversal potential in a low $Cl^-$ bath solution and pharmacological properties suggest that this is a $Ca^{2+}$-independent, volume activated $Cl^-$ current. We conclude therefore that volume activated $Cl^-$ channels play a putative role in RVD in human salivary gland acinar cells.

Keywords

References

  1. Arreola J, Park, K, Melvin JE, Begenisich T. Three distinct chloride channels control anion movements in rat parotid acinar cells. J Physiol. 1996; 490(2): 351-362 https://doi.org/10.1113/jphysiol.1996.sp021149
  2. Elliott AC. Effects of cell swelling on intracellular calcium in isolated rat lacrimal acinar cells. J Physiol. 1994; 477: 9P
  3. Fatherazi S, Izutsu KT, Wellner RB, Belton CM. Hypotonically activated chloride current in HSG cells. J Membr Biol. 1994; 142(2):181-193
  4. Foskett JK, Melvin JE. Activation of salivary secretion : coupling of $[Ca^{2+}]_{i}$ and cell volume. Science. 1989; 244:1582-1585 https://doi.org/10.1126/science.2500708
  5. Foskett JK, Wong MMM, Sue-A-Quan G, Robertson MA. Isosmotic modulation of cell volume and intracellular ion activities during stimulation of single exocrine cells. J Exp Zool. 1994; 268,104-110 https://doi.org/10.1002/jez.1402680206
  6. Kotera T, Brown PD. Calcium-dependent chloride current activated by hyposmotic stress in rat lacrimal acinar cells. J Membr Biol. 1993; 134(1):67-74
  7. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ. Improved patch clamp techniques for high resolution current recording from cells and cell free membrane patches. Pfuegers Arch. 1981; 391:85-100 https://doi.org/10.1007/BF00656997
  8. Kim SH, Cho YK, Chung KM, Kim KN. Purinergic Receptors Play Roles in Secretion of Rat von Ebner Salivary Gland. Int J Oral Biol. 2006; 31(4):.141-148
  9. Majid A, Brown PD, Best L, Park K. Expression of volume sensitive $Cl^{-}$ channels and ClC-3 in acinar cells isolated from the rat lacrimal gland and submandibular salivary gland. J Physiol. 2001; 534: 409-421 https://doi.org/10.1111/j.1469-7793.2001.00409.x
  10. McCarty NA, ONeil RG. Calcium dependent control of volume regulation in renal proximal tubule cells: I. Swellingactivated entry and $Ca^{2+}$ release. J Membr Biol. 1991; 123, 149-160 https://doi.org/10.1007/BF01998085
  11. Montrose-Rafizadeh C, Guggino WB. Role of intracellular calcium in volume regulation by rabbit medullary thick ascending limb cells. Am J Physiol. 1991; 260: F402-409
  12. Moran A, Turner RJ. Secretagogue-induced RVD in HSY cells is due to $K^{+}$ channels activated by $Ca^{2+}$ and protein kinase C. Am J Physiol. 1993; 265(5 Pt 1):C1405-11 https://doi.org/10.1152/ajpcell.1993.265.5.C1405
  13. Nakahari T, Murakami M, Yoshida H, Miyamoto M, Sohma Y, Imai Y. Decrease in rat submandibular acinar cell volume during Ach stimulation. Am J Physiol. 1990; 258:G878-G886
  14. Negulescu PA, Munck B, Machen TE. Volume-sensitive Ca influx and release from intracellular pools in gastric parietal cells. Am J Physiol. 1992; 263: C584-C589
  15. Park K, Beck JS, Douglas IJ, Brown PD. $Ca^{2+}$-activated $K^{+}$ channels are involved in regulatory volume decrease in acinar cells isolated from the rat lacrimal gland. J Membr Biol. 1994; 141:193-201
  16. Park K. Swelling-induced activation of K+ channels is mediated by $Ca^{2+}$ influx in rat lacrimal acinar cells. J Oral Biol. 1994;18(1): 55-62,1994
  17. Park K, Brown PD. Intracellular pH modulates the activity of chloride channels in isolated lacrimal gland acinar cells. Am J Physiol 1995; 268: C647-C650 https://doi.org/10.1152/ajpcell.1995.268.3.C647
  18. Park K, Case RM, Brown PD. Identification and regulation of $K^{+}$ and $Cl^{-}$ channels in human parotid acinar cells. Arch Oral Biol. 2001; 46(9):801-810 https://doi.org/10.1016/S0003-9969(01)00047-4
  19. Park K, Lee S, Elliott AC, Kim JS, Lee JH. Swelling-induced $Ca^{2+}$ release from intracellular calcium stores in rat submandibular gland acinar cells. J Membr Biol. 2002;186 (3):165-176 https://doi.org/10.1007/s00232-001-0144-8
  20. Speake T, Douglas IJ, Brown PD. The role of calcium in the volume regulation of rat lacrimal acinar cells. J Memb Biol 1998; 164: 283-291 https://doi.org/10.1007/s002329900413
  21. Strange K, Emma F, Jackson PS. Cellular and molecular physiology of volume sensitive anion channels. Am J Physiol. 1996; 270:C711-C730