Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Optimal Culture Conditions of Prodigiosin-like Pigment Produced by Zooshikella sp. JE-34

Zooshikella sp. JE-34가 생산하는 Prodigiosin계 색소의 분리 및 최적 배양조건

  • Kim, Ju-Sang (Department of Aquatic Life Medicine & Marine and Environmental research Institute, jeju National University) ;
  • Kim, Man-Chul (Department of Aquatic Life Medicine & Marine and Environmental research Institute, jeju National University) ;
  • Lee, Kyeong-Jun (Department of Marine Life Science, jeju National University) ;
  • Heo, Moon-Soo (Department of Aquatic Life Medicine & Marine and Environmental research Institute, jeju National University)
  • 김주상 (제주대학교 수산생명의학과 해양과환경연구소) ;
  • 김만철 (제주대학교 수산생명의학과 해양과환경연구소) ;
  • 이경준 (제주대학교 해양생명과학과) ;
  • 허문수 (제주대학교 수산생명의학과 해양과환경연구소)
  • Published : 2009.09.28
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Abstract

A bacterial strain, JE-34, producing a high concentration of red pigment was isolated from a sediment in East China Sea. It was identified as Zooshikella sp. JE-34 based on the 16S rRNA gene sequence analysis. The red pigment was purified by solvent extraction and HPLC was identified as prodigiosin-like compound. Nutritional and cultural conditions were optimized for the production of prodigiosin-like pigment in the flask level. Optimal culture conditions were at initial medium pH $6.0{\sim}7.0$, $30^{\circ}C$ and 4 days incubation. For carbon and, nitrogen sources were soluble starch and malt extract.

본 연구는 해양유래의 특이색소미생물을 선발하여 미생물의 특성 및 이차대사산물의 생리활성 탐색을 통해 새로운 생물자원개발을 위해 수행되었다. 본 연구에 사용된 prodigiosin 유사 색소를 생산하는 균주 JE-34는 동중국해 퇴적층으로부터 분리되었으며 Zooshikella sp. JE-34으로 명명하였다. Zooshikella sp. JE-34는 적색색소를 다량 생산하였으며 대표적인 prodigiosin 생산균주인 Serratia marcscens의 색소추출액과 분광학적 특성 및 HPLC 분석을 통해 JE-34가 생산하는 색소가 prodigiosin 유사색소로 판단되었다. JE-34 균주의 배지조성에 따른 생육도 및 pigment 생합성의 변화를 알아보기 위해 탄소원, 질소원, 무기염류를 첨가하며 배양한 결과 탄소원으로는 soluble starch, 질 소원으로는 malt extract, 무기염류로는 $Na_2HPO_4$에서 최적의 pigment를 생산하였다. Zooshikella sp. JE-34는 prodigiosin의 생합성에 있어 대표적인 생산자인 Serratia marcscens와 마찬가지로 우수한 pigment 생합성량을 보여 prodigiosin의 생합성의 생물자원으로서 의의가 있다고 사료된다.

Keywords

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