Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Analysis of Endospore-forming Bacteria or Nitrogen-fixing Bacteria Community Isolated from Plants Rhizosphere in Dokdo Island

독도서식 식물근권에서 분리한 포자형성세균과 질소고정세균의 군집구조 분석

  • Jeon, Seon-Ae (School of Life Sciences, and Research Institute for Dokdo & Ulleungdo Islands, Kyungpook National University) ;
  • Sung, Hye-Ri (School of Life Sciences, and Research Institute for Dokdo & Ulleungdo Islands, Kyungpook National University) ;
  • Park, Yu-Mi (School of Life Sciences, and Research Institute for Dokdo & Ulleungdo Islands, Kyungpook National University) ;
  • Pak, Jae-Hong (School of Life Sciences, and Research Institute for Dokdo & Ulleungdo Islands, Kyungpook National University) ;
  • Ghim, Sa-Youl (School of Life Sciences, and Research Institute for Dokdo & Ulleungdo Islands, Kyungpook National University)
  • 전선애 (경북대학교 생명과학부, 경북대학교 울릉도 독도연구소) ;
  • 성혜리 (경북대학교 생명과학부, 경북대학교 울릉도 독도연구소) ;
  • 박유미 (경북대학교 생명과학부, 경북대학교 울릉도 독도연구소) ;
  • 박재홍 (경북대학교 생명과학부, 경북대학교 울릉도 독도연구소) ;
  • 김사열 (경북대학교 생명과학부, 경북대학교 울릉도 독도연구소)
  • Published : 2009.09.28
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Abstract

Bacteria were isolated from roots of plants belonging to family Solanaceae and Gramineae, inhabited in Dokdo island. Fifty six endospore-forming bacteria grown on tryptic soy broth (TSB) agar medium and 23 nitrogen-fixing bacteria (NFB) grown on nitrogen free agar medium were isolated, respectively. The isolates were partially identified by analyzing the 16S rDNA and categorized into phylogenetic groups. The 16S rDNA sequences of each identified isolates were compared with sequences of each type strains to analyze phylogenetic relationship by phylogenetic tree. As a result, endospore-forming bacteria and nitrogen-fixing bacteria were classified into 4 and 6 lineage groups, respectively. Among these isolated, 18 were presumed to be novel species candidates based on the similarity (lower than 98%) analysis of the l6S rDNA sequences.

Keywords

References

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